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Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection 期刊论文
BIOSENSORS & BIOELECTRONICS, 2022, 卷号: 211, 页码: 6
Authors:  Wei, Yangdao;  Tao, Zhenzhen;  Wan, Lu;  Zong, Chengli;  Wu, Jiajia;  Tan, Xiao;  Wang, Buhua;  Guo, Zixuan;  Zhang, Ling;  Yuan, Haoyu;  Wang, Peng;  Yang, Zhiqing;  Wan, Yi
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CRISPR-Cas  Bacteria diagnostic  Staphylococcus aureus  
Electroporation-Based CRISPR/Cas9 Mosaic Mutagenesis of beta-Tubulin in the Cultured Oyster 期刊论文
FRONTIERS IN MARINE SCIENCE, 2022, 卷号: 9, 页码: 12
Authors:  Chan, Jiulin;  Zhang, Wei;  Xu, Yue;  Xue, Yu;  Zhang, Linlin
Adobe PDF(11733Kb)  |  Favorite  |  View/Download:29/0  |  Submit date:2022/07/18
mosaic mutagenesis  CRISPR  Cas9  long deletion  gene editing  gene knockout  aquaculture breeding  
Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System 期刊论文
JOURNAL OF OCEAN UNIVERSITY OF CHINA, 2021, 卷号: 20, 期号: 6, 页码: 1528-1536
Authors:  Tan Xungang;  Wang Ling;  Wu Zhihao;  Jiao Shuang;  Wang Lijuan;  Zou Yuxia;  Jiang Jingteng;  You Feng
Adobe PDF(945Kb)  |  Favorite  |  View/Download:60/0  |  Submit date:2022/01/05
CRISPR  Cas9  gsdf  microinjection  myomaker  needle with brake  olive flounder  
trans Single-Stranded DNA Cleavage via CRISPR/Cas14a1 Activated by Target RNA without Destruction 期刊论文
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2021, 页码: 8
Authors:  Wei, Yangdao;  Yang, Zhiqing;  Zong, Chengli;  Wang, Buhua;  Ge, Xiaolin;  Tan, Xiao;  Liu, Xin;  Tao, Zhenzhen;  Wang, Peng;  Ma, Chunxin;  Wan, Yi;  Li, Jinghong
Adobe PDF(2146Kb)  |  Favorite  |  View/Download:50/0  |  Submit date:2021/12/01
CRISPR-Cas14a1  DNA cleavage  pathogenic bacteria  RNA  RNA detection platform  
Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform 期刊论文
BIOSENSORS & BIOELECTRONICS, 2021, 卷号: 185, 页码: 5
Authors:  Song, Fengge;  Wei, Yangdao;  Wang, Peng;  Ge, Xiaolin;  Li, Chaoyang;  Wang, Aimin;  Yang, Zhiqing;  Wan, Yi;  Li, Jinghong
Adobe PDF(2476Kb)  |  Favorite  |  View/Download:161/1  |  Submit date:2021/06/24
Bacterial diagnostics  CRISPR-Cas14a  Tag-specific primer extension  Magneto-DNA  
CRISPR/Cas9-mediated mutagenesis reveals the roles of calaxin in gastropod larval cilia 期刊论文
GENE, 2021, 卷号: 787, 页码: 10
Authors:  Huan, Pin;  Cui, Menglu;  Wang, Qian;  Liu, Baozhong
Adobe PDF(10009Kb)  |  Favorite  |  View/Download:112/0  |  Submit date:2021/06/04
Calaxin  CRISPR  Cas9  Gene editing  Mollusk  
牙鲆基因编辑技术构建及其应用的初步研究 学位论文
理学博士, 中国科学院海洋研究所: 中国科学院大学, 2021
Authors:  王凌
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牙鲆,显微注射及电转,CRISPR/Cas9系统,PGC迁移因子,dnali1  
Asgard archaea in the haima cold seep: Spatial distribution and genomic insights 期刊论文
DEEP-SEA RESEARCH PART I-OCEANOGRAPHIC RESEARCH PAPERS, 2021, 卷号: 170, 页码: 10
Authors:  Lu, Rui;  Gao, Zhao-Ming;  Li, Wen-Li;  Wei, Zhan-Fei;  Wei, Tao-Shu;  Huang, Jiao-Mei;  Li, Meng;  Tao, Jun;  Wang, Hong-Bin;  Wang, Yong
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Asgard  Cold seep  CRISPR-Cas system  Gerdarchaeota  Heimdallarchaeota  
Targeted mutagenesis in the olive flounder (Paralichthys olivaceus) using the CRISPR/Cas9 system with electroporation 期刊论文
BIOLOGIA, 2021, 页码: 8
Authors:  Wang, Ling;  Tan, Xungang;  Wu, Zhihao;  Wang, Lijuan;  Jiao, Shuang;  Zou, Yuxia;  Ji, Guanglei;  You, Feng
Adobe PDF(1088Kb)  |  Favorite  |  View/Download:82/0  |  Submit date:2021/04/21
Genome editing  CRISPR/Cas9 system  Electroporation  Myomaker and gsdf  Olive flounder (Paralichthys olivaceus)  
CRISPR/Cas9-mediated mutation reveals Pax6 is essential for development of the compound eye in Decapoda Exopalaemon carinicauda 期刊论文
DEVELOPMENTAL BIOLOGY, 2020, 卷号: 465, 期号: 2, 页码: 157-167
Authors:  Gao, Yi;  Zhang, Xiaoxi;  Zhang, Xiaojun;  Yuan, Jianbo;  Xiang, Jianhai;  Li, Fuhua
Adobe PDF(6053Kb)  |  Favorite  |  View/Download:61/0  |  Submit date:2021/04/12
Development  Compound eye  Pax6  CRISPR/Cas9  Crustaceans