CRISPR/Cas9-mediated gene mutation of <i>EcIAG</i> leads to sex reversal in the male ridgetail white prawn <i>Exopalaemon carinicauda</i>

doi:10.3389/fendo.2023.1266641

IOCAS-IR > 实验海洋生物学重点实验室

	CRISPR/Cas9-mediated gene mutation of EcIAG leads to sex reversal in the male ridgetail white prawn Exopalaemon carinicauda
	Miao, Miao 1,2,3; Li, Shihao 1,2,4; Yuan, Jianbo 1,2,4; Liu, Peipei 1,2,3; Fang, Xiaochen 1,2,3; Zhang, Chengsong1,2,4 ; Zhang, Xiaojun 1,2,4; Li, Fuhua 1,2,4,5
	2023-11-21
发表期刊	FRONTIERS IN ENDOCRINOLOGY
ISSN	1664-2392
卷号	14 页码:11
通讯作者	Li, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
摘要	In the culture of crustaceans, most species show sexual dimorphism. Monosex culture is an effective approach to achieve high yield and economic value, especially for decapods of high value. Previous studies have developed some sex control strategies such as manual segregation, manipulation of male androgenic gland and knockdown of the male sexual differentiation switch gene encoding insulin-like androgenic gland hormone (IAG) in decapods. However, these methods could not generate hereditable changes. Genetic manipulation to achieve sex reversal individuals is absent up to now. In the present study, the gene encoding IAG (EcIAG) was identified in the ridgetail white prawn Exopalaemon carinicauda. Sequence analysis showed that EcIAG encoded conserved amino acid structure like IAGs in other decapod species. CRISPR/Cas9-mediated genome editing technology was used to knock out EcIAG. Two sgRNAs targeting the second exon of EcIAG were designed and microinjected into the prawn zygotes or the embryos at the first cleavage with commercial Cas9 protein. EcIAG in three genetic males was knocked out in both chromosome sets, which successfully generated sex reversal and phenotypic female characters. The results suggest that CRISPR/Cas9-mediated genome editing technology is an effective way to develop sex manipulation technology and contribute to monosex aquaculture in crustaceans.
关键词	insulin-like androgenic gland hormone Exopalaemon carinicauda CRISPR/Cas9 sex reversal gene editing
DOI	10.3389/fendo.2023.1266641
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2022YFD2401400] ; Earmarked fund for CARS-48 ; Taishan Scholars Program
WOS研究方向	Endocrinology & Metabolism
WOS类目	Endocrinology & Metabolism
WOS记录号	WOS:001118980000001
出版者	FRONTIERS MEDIA SA
WOS关键词	EXPRESSION ANALYSIS ; CDNA CLONING ; FEMALE ; MONOSEX ; HORMONE ; GROWTH ; IDENTIFICATION ; HOMOLOG
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184107
专题	实验海洋生物学重点实验室
通讯作者	Li, Shihao; Li, Fuhua
作者单位	1.Chinese Acad Sci, CAS, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 5.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Miao, Miao,Li, Shihao,Yuan, Jianbo,et al. CRISPR/Cas9-mediated gene mutation of EcIAG leads to sex reversal in the male ridgetail white prawn Exopalaemon carinicauda[J]. FRONTIERS IN ENDOCRINOLOGY,2023,14:11.
APA	Miao, Miao.,Li, Shihao.,Yuan, Jianbo.,Liu, Peipei.,Fang, Xiaochen.,...&Li, Fuhua.(2023).CRISPR/Cas9-mediated gene mutation of EcIAG leads to sex reversal in the male ridgetail white prawn Exopalaemon carinicauda.FRONTIERS IN ENDOCRINOLOGY,14,11.
MLA	Miao, Miao,et al."CRISPR/Cas9-mediated gene mutation of EcIAG leads to sex reversal in the male ridgetail white prawn Exopalaemon carinicauda".FRONTIERS IN ENDOCRINOLOGY 14(2023):11.

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