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Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System
Tan Xungang1,2; Wang Ling1,2,3; Wu Zhihao1,2; Jiao Shuang1,2; Wang Lijuan1,2; Zou Yuxia1,2; Jiang Jingteng4,5; You Feng1,2
2021-12-01
Source PublicationJOURNAL OF OCEAN UNIVERSITY OF CHINA
ISSN1672-5182
Volume20Issue:6Pages:1528-1536
Corresponding AuthorTan Xungang(tanx@qdio.ac.cn) ; You Feng(youfeng@qdio.ac.cn)
AbstractThe whole-genome sequence of the olive flounder (Paralichthys olivaceus) provides a basis for gene functional analyses, which is important for the aquaculture industry. Understanding gene function will help us to select better economic traits such as fast growth and better culture conditions, which further will increase the aquaculture output. Gene knockout is an important reverse genetics approach for in vivo studies of gene function. In this study, the CRISPR/Cas9 genome editing method with a microinjection system using a simple braked needle was employed in olive flounder. After injection in embryos, green fluorescent protein expression was detected in 40% of larvae. The proportion of normal-hatched larvae was approximately 50%. Different mutations, including short indels and fragment deletions, were found in our test genes gsdf and myomaker. Additionally, we detected more than one mutation in a single larva. In summary, our microinjection technique and CRISPR/Cas9 can be applied to study gene functions in olive flounder.
KeywordCRISPR Cas9 gsdf microinjection myomaker needle with brake olive flounder
DOI10.1007/s11802-021-4772-6
Indexed BySCI
Language英语
WOS Research AreaOceanography
WOS SubjectOceanography
WOS IDWOS:000720813700021
PublisherOCEAN UNIV CHINA
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/177213
Collection实验海洋生物学重点实验室
Corresponding AuthorTan Xungang; You Feng
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab ForMarine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Weihai Fishery Technol Promot Stn, Weihai 264200, Peoples R China
5.Shenghang Aquat Sci & Technol Co Ltd, Weihai 264200, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Tan Xungang,Wang Ling,Wu Zhihao,et al. Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2021,20(6):1528-1536.
APA Tan Xungang.,Wang Ling.,Wu Zhihao.,Jiao Shuang.,Wang Lijuan.,...&You Feng.(2021).Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System.JOURNAL OF OCEAN UNIVERSITY OF CHINA,20(6),1528-1536.
MLA Tan Xungang,et al."Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System".JOURNAL OF OCEAN UNIVERSITY OF CHINA 20.6(2021):1528-1536.
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