Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Antihypertensive Effects of Two Novel Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides from Gracilariopsis lemaneiformis (Rhodophyta) in Spontaneously Hypertensive Rats (SHRs) | |
Deng, Zhenzhen1,2,3,4; Liu, Yingjuan1,2,3,4; Wang, Jing1,2,3,4 ; Wu, Suhuang1,2,3,4; Geng, Lihua1,2,3,4; Sui, Zhenghong5; Zhang, Quanbin1,2,3,4
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| 2018-09-01 | |
| Source Publication | MARINE DRUGS
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| ISSN | 1660-3397 |
| Volume | 16Issue:9Pages:16 |
| Corresponding Author | Sui, Zhenghong(suizhengh@ouc.edu.cn) ; Zhang, Quanbin(qbzhang@qdio.ac.cn) |
| Abstract | A variety of biologically active products have been isolated from Gracilariopsis lemaneiformis. In the present study, two novel angiotensin-converting enzyme (ACE) inhibitory peptides, FQIN [M(O)] CILR, and TGAPCR, were screened and identified from G. lemaneiformis protein hydrolysates by LC-MS/MS. The IC50 values of FQIN [M(O)] CILR and TGAPCR were 9.64 +/- 0.36 M and 23.94 +/- 0.82 M, respectively. In the stability study, both peptides showed stabilities of pH, temperature, simulated gastrointestinal digestion, and ACE hydrolysis. The Lineweaver-Burk plot showed that the two peptides were noncompetitive inhibitors of ACE. Molecular docking simulated the intermolecular interactions of two peptides and ACE, and the two peptides formed hydrogen bonds with the active pockets of ACE. However, FQIN [M(O)] CILR was more closely linked to the active pockets of ACE, thereby exerting better ACE inhibition. Spontaneously hypertensive rats (SHRs) were studied with an oral dose of 10 mg/kg body weight. Both peptides reduced systolic blood pressure (SBP) and diastolic blood pressure (DBP) in SHRs, of which FQIN [M(O)] CILR was able to reduce the systolic blood pressure by 34 mmHg (SBP) (p < 0.05). Therefore, FQIN [M(O)] CILR was an excellent ACE inhibitory peptide. |
| Keyword | Gracilariopsis lemaneiformis ACE-inhibitory activity peptide molecular docking SHRs |
| DOI | 10.3390/md16090299 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Fujian STS project of Chinese Academy of Sciences ; Science and Technology project of Fujian Province[2017T3015] ; Science and Technology project of Fujian Province[2017T3013] ; Key Research and Development Project of Shandong Province[2016YYSP002] ; Youth Innovation Promotion Association of CAS[2016190] ; K.C. Wong Education Foundation, CAS |
| WOS Research Area | Pharmacology & Pharmacy |
| WOS Subject | Chemistry, Medicinal |
| WOS ID | WOS:000445636600010 |
| Publisher | MDPI |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/160350 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Sui, Zhenghong; Zhang, Quanbin |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Tech, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 5.Ocean Univ China, Coll Marine Life Sci, Qingdao 266071, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Deng, Zhenzhen,Liu, Yingjuan,Wang, Jing,et al. Antihypertensive Effects of Two Novel Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides from Gracilariopsis lemaneiformis (Rhodophyta) in Spontaneously Hypertensive Rats (SHRs)[J]. MARINE DRUGS,2018,16(9):16. |
| APA | Deng, Zhenzhen.,Liu, Yingjuan.,Wang, Jing.,Wu, Suhuang.,Geng, Lihua.,...&Zhang, Quanbin.(2018).Antihypertensive Effects of Two Novel Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides from Gracilariopsis lemaneiformis (Rhodophyta) in Spontaneously Hypertensive Rats (SHRs).MARINE DRUGS,16(9),16. |
| MLA | Deng, Zhenzhen,et al."Antihypertensive Effects of Two Novel Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides from Gracilariopsis lemaneiformis (Rhodophyta) in Spontaneously Hypertensive Rats (SHRs)".MARINE DRUGS 16.9(2018):16. |
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| File Name/Size | DocType | Version | Access | License | ||
| Antihypertensive Eff（2987KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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