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Antihypertensive Effects of Two Novel Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides from Gracilariopsis lemaneiformis (Rhodophyta) in Spontaneously Hypertensive Rats (SHRs)
Deng, Zhenzhen1,2,3,4; Liu, Yingjuan1,2,3,4; Wang, Jing1,2,3,4; Wu, Suhuang1,2,3,4; Geng, Lihua1,2,3,4; Sui, Zhenghong5; Zhang, Quanbin1,2,3,4
2018-09-01
Source PublicationMARINE DRUGS
ISSN1660-3397
Volume16Issue:9Pages:16
Corresponding AuthorSui, Zhenghong(suizhengh@ouc.edu.cn) ; Zhang, Quanbin(qbzhang@qdio.ac.cn)
AbstractA variety of biologically active products have been isolated from Gracilariopsis lemaneiformis. In the present study, two novel angiotensin-converting enzyme (ACE) inhibitory peptides, FQIN [M(O)] CILR, and TGAPCR, were screened and identified from G. lemaneiformis protein hydrolysates by LC-MS/MS. The IC50 values of FQIN [M(O)] CILR and TGAPCR were 9.64 +/- 0.36 M and 23.94 +/- 0.82 M, respectively. In the stability study, both peptides showed stabilities of pH, temperature, simulated gastrointestinal digestion, and ACE hydrolysis. The Lineweaver-Burk plot showed that the two peptides were noncompetitive inhibitors of ACE. Molecular docking simulated the intermolecular interactions of two peptides and ACE, and the two peptides formed hydrogen bonds with the active pockets of ACE. However, FQIN [M(O)] CILR was more closely linked to the active pockets of ACE, thereby exerting better ACE inhibition. Spontaneously hypertensive rats (SHRs) were studied with an oral dose of 10 mg/kg body weight. Both peptides reduced systolic blood pressure (SBP) and diastolic blood pressure (DBP) in SHRs, of which FQIN [M(O)] CILR was able to reduce the systolic blood pressure by 34 mmHg (SBP) (p < 0.05). Therefore, FQIN [M(O)] CILR was an excellent ACE inhibitory peptide.
KeywordGracilariopsis lemaneiformis ACE-inhibitory activity peptide molecular docking SHRs
DOI10.3390/md16090299
Indexed BySCI
Language英语
Funding ProjectFujian STS project of Chinese Academy of Sciences ; Science and Technology project of Fujian Province[2017T3015] ; Science and Technology project of Fujian Province[2017T3013] ; Key Research and Development Project of Shandong Province[2016YYSP002] ; Youth Innovation Promotion Association of CAS[2016190] ; K.C. Wong Education Foundation, CAS
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal
WOS IDWOS:000445636600010
PublisherMDPI
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/160350
Collection实验海洋生物学重点实验室
Corresponding AuthorSui, Zhenghong; Zhang, Quanbin
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Tech, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
5.Ocean Univ China, Coll Marine Life Sci, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Deng, Zhenzhen,Liu, Yingjuan,Wang, Jing,et al. Antihypertensive Effects of Two Novel Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides from Gracilariopsis lemaneiformis (Rhodophyta) in Spontaneously Hypertensive Rats (SHRs)[J]. MARINE DRUGS,2018,16(9):16.
APA Deng, Zhenzhen.,Liu, Yingjuan.,Wang, Jing.,Wu, Suhuang.,Geng, Lihua.,...&Zhang, Quanbin.(2018).Antihypertensive Effects of Two Novel Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides from Gracilariopsis lemaneiformis (Rhodophyta) in Spontaneously Hypertensive Rats (SHRs).MARINE DRUGS,16(9),16.
MLA Deng, Zhenzhen,et al."Antihypertensive Effects of Two Novel Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides from Gracilariopsis lemaneiformis (Rhodophyta) in Spontaneously Hypertensive Rats (SHRs)".MARINE DRUGS 16.9(2018):16.
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