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| Antihypertensive Effects of Two Novel Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides from Gracilariopsis lemaneiformis (Rhodophyta) in Spontaneously Hypertensive Rats (SHRs) | |
Deng, Zhenzhen1,2,3,4; Liu, Yingjuan1,2,3,4; Wang, Jing1,2,3,4 ; Wu, Suhuang1,2,3,4; Geng, Lihua1,2,3,4; Sui, Zhenghong5; Zhang, Quanbin1,2,3,4
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| 2018-09-01 | |
| 发表期刊 | MARINE DRUGS
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| ISSN | 1660-3397 |
| 卷号 | 16期号:9页码:16 |
| 通讯作者 | Sui, Zhenghong(suizhengh@ouc.edu.cn) ; Zhang, Quanbin(qbzhang@qdio.ac.cn) |
| 摘要 | A variety of biologically active products have been isolated from Gracilariopsis lemaneiformis. In the present study, two novel angiotensin-converting enzyme (ACE) inhibitory peptides, FQIN [M(O)] CILR, and TGAPCR, were screened and identified from G. lemaneiformis protein hydrolysates by LC-MS/MS. The IC50 values of FQIN [M(O)] CILR and TGAPCR were 9.64 +/- 0.36 M and 23.94 +/- 0.82 M, respectively. In the stability study, both peptides showed stabilities of pH, temperature, simulated gastrointestinal digestion, and ACE hydrolysis. The Lineweaver-Burk plot showed that the two peptides were noncompetitive inhibitors of ACE. Molecular docking simulated the intermolecular interactions of two peptides and ACE, and the two peptides formed hydrogen bonds with the active pockets of ACE. However, FQIN [M(O)] CILR was more closely linked to the active pockets of ACE, thereby exerting better ACE inhibition. Spontaneously hypertensive rats (SHRs) were studied with an oral dose of 10 mg/kg body weight. Both peptides reduced systolic blood pressure (SBP) and diastolic blood pressure (DBP) in SHRs, of which FQIN [M(O)] CILR was able to reduce the systolic blood pressure by 34 mmHg (SBP) (p < 0.05). Therefore, FQIN [M(O)] CILR was an excellent ACE inhibitory peptide. |
| 关键词 | Gracilariopsis lemaneiformis ACE-inhibitory activity peptide molecular docking SHRs |
| DOI | 10.3390/md16090299 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Fujian STS project of Chinese Academy of Sciences ; Science and Technology project of Fujian Province[2017T3015] ; Science and Technology project of Fujian Province[2017T3013] ; Key Research and Development Project of Shandong Province[2016YYSP002] ; Youth Innovation Promotion Association of CAS[2016190] ; K.C. Wong Education Foundation, CAS ; Fujian STS project of Chinese Academy of Sciences ; Science and Technology project of Fujian Province[2017T3015] ; Science and Technology project of Fujian Province[2017T3013] ; Key Research and Development Project of Shandong Province[2016YYSP002] ; Youth Innovation Promotion Association of CAS[2016190] ; K.C. Wong Education Foundation, CAS |
| WOS研究方向 | Pharmacology & Pharmacy |
| WOS类目 | Chemistry, Medicinal |
| WOS记录号 | WOS:000445636600010 |
| 出版者 | MDPI |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/160350 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Sui, Zhenghong; Zhang, Quanbin |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Tech, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 5.Ocean Univ China, Coll Marine Life Sci, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Deng, Zhenzhen,Liu, Yingjuan,Wang, Jing,et al. Antihypertensive Effects of Two Novel Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides from Gracilariopsis lemaneiformis (Rhodophyta) in Spontaneously Hypertensive Rats (SHRs)[J]. MARINE DRUGS,2018,16(9):16. |
| APA | Deng, Zhenzhen.,Liu, Yingjuan.,Wang, Jing.,Wu, Suhuang.,Geng, Lihua.,...&Zhang, Quanbin.(2018).Antihypertensive Effects of Two Novel Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides from Gracilariopsis lemaneiformis (Rhodophyta) in Spontaneously Hypertensive Rats (SHRs).MARINE DRUGS,16(9),16. |
| MLA | Deng, Zhenzhen,et al."Antihypertensive Effects of Two Novel Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides from Gracilariopsis lemaneiformis (Rhodophyta) in Spontaneously Hypertensive Rats (SHRs)".MARINE DRUGS 16.9(2018):16. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Antihypertensive Eff（2987KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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