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Sulfated Hetero-Polysaccharides Protect SH-SY5Y Cells from H2O2-Induced Apoptosis by Affecting the PI3K/Akt Signaling Pathway
Wang, Jing1,4; Liu, Huaide2; Zhang, Xuan3; Li, Xinpeng1,4; Geng, Lihua1,4; Zhang, Hong1,4; Zhang, Quanbin1,4
2017-04-01
发表期刊MARINE DRUGS
卷号15期号:4
文章类型Article
摘要Parkinson's disease (PD) is one of the most common neurodegenerative diseases. Recent studies suggest that sulfated hetero-polysaccharides (UF) protect against developing PD. However, the detailed mechanisms of how UF suppress neuronal death have not been fully elucidated. We investigated the cytoprotective mechanisms of UF using human dopaminergic neuroblastoma SH-SY5Y cells as a PD model. UF prevented H2O2-induced apoptotic cell death in SH-SY5Y cells in a dose-dependent manner. An examination of the PI3K/Akt upstream pathway revealed that UF-pretreated cells showed a decreased relative density of Akt, PI3K, and TrkA, and increased the phosphorylation of Akt, PI3K, and NGF; the PI3K inhibitor, LY294002, partially prevented this effect. An examination of the PI3K/Akt downstream pathway revealed the increased expression of the apoptosis-associated markers Bax, p53, CytC, and GSK3 beta(,) and the decreased expression of Bcl-2 in UF-treated cells. UF-treated cells also exhibited decreased caspase-3, caspase-8, and caspase-9 activities, which induced cell apoptosis. Our results demonstrate that UF affect the PI3K/Akt pathway, as well as downstream signaling. Therefore, the UF-mediated activation of PI3K/Akt could provide a new potential therapeutic strategy for neurodegenerative diseases associated with oxidative injury. These findings contribute to a better understanding of the critical roles of UF in the treatment of PD.
关键词Sulfated Hetero-polysaccharides Pi3k/akt Phosphorylate Sh-sy5y Oxidative Stress
DOI10.3390/md15040110
收录类别SCI
语种英语
WOS记录号WOS:000404228300026
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/137144
专题海洋生物技术研发中心
实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
2.Nantong Univ, Sch Life Sci, Seyuan Rd 9, Nantong 226019, Peoples R China
3.Taian City Cent Hosp, Tai An 271000, Shandong, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
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Wang, Jing,Liu, Huaide,Zhang, Xuan,et al. Sulfated Hetero-Polysaccharides Protect SH-SY5Y Cells from H2O2-Induced Apoptosis by Affecting the PI3K/Akt Signaling Pathway[J]. MARINE DRUGS,2017,15(4).
APA Wang, Jing.,Liu, Huaide.,Zhang, Xuan.,Li, Xinpeng.,Geng, Lihua.,...&Zhang, Quanbin.(2017).Sulfated Hetero-Polysaccharides Protect SH-SY5Y Cells from H2O2-Induced Apoptosis by Affecting the PI3K/Akt Signaling Pathway.MARINE DRUGS,15(4).
MLA Wang, Jing,et al."Sulfated Hetero-Polysaccharides Protect SH-SY5Y Cells from H2O2-Induced Apoptosis by Affecting the PI3K/Akt Signaling Pathway".MARINE DRUGS 15.4(2017).
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