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Sulfated Hetero-Polysaccharides Protect SH-SY5Y Cells from H2O2-Induced Apoptosis by Affecting the PI3K/Akt Signaling Pathway
Wang, Jing1,4; Liu, Huaide2; Zhang, Xuan3; Li, Xinpeng1,4; Geng, Lihua1,4; Zhang, Hong1,4; Zhang, Quanbin1,4
2017-04-01
Source PublicationMARINE DRUGS
Volume15Issue:4
SubtypeArticle
AbstractParkinson's disease (PD) is one of the most common neurodegenerative diseases. Recent studies suggest that sulfated hetero-polysaccharides (UF) protect against developing PD. However, the detailed mechanisms of how UF suppress neuronal death have not been fully elucidated. We investigated the cytoprotective mechanisms of UF using human dopaminergic neuroblastoma SH-SY5Y cells as a PD model. UF prevented H2O2-induced apoptotic cell death in SH-SY5Y cells in a dose-dependent manner. An examination of the PI3K/Akt upstream pathway revealed that UF-pretreated cells showed a decreased relative density of Akt, PI3K, and TrkA, and increased the phosphorylation of Akt, PI3K, and NGF; the PI3K inhibitor, LY294002, partially prevented this effect. An examination of the PI3K/Akt downstream pathway revealed the increased expression of the apoptosis-associated markers Bax, p53, CytC, and GSK3 beta(,) and the decreased expression of Bcl-2 in UF-treated cells. UF-treated cells also exhibited decreased caspase-3, caspase-8, and caspase-9 activities, which induced cell apoptosis. Our results demonstrate that UF affect the PI3K/Akt pathway, as well as downstream signaling. Therefore, the UF-mediated activation of PI3K/Akt could provide a new potential therapeutic strategy for neurodegenerative diseases associated with oxidative injury. These findings contribute to a better understanding of the critical roles of UF in the treatment of PD.
KeywordSulfated Hetero-polysaccharides Pi3k/akt Phosphorylate Sh-sy5y Oxidative Stress
DOI10.3390/md15040110
Indexed BySCI
Language英语
WOS IDWOS:000404228300026
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/137144
Collection海洋生物技术研发中心
实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
2.Nantong Univ, Sch Life Sci, Seyuan Rd 9, Nantong 226019, Peoples R China
3.Taian City Cent Hosp, Tai An 271000, Shandong, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
First Author Affilication中国科学院海洋研究所
Recommended Citation
GB/T 7714
Wang, Jing,Liu, Huaide,Zhang, Xuan,et al. Sulfated Hetero-Polysaccharides Protect SH-SY5Y Cells from H2O2-Induced Apoptosis by Affecting the PI3K/Akt Signaling Pathway[J]. MARINE DRUGS,2017,15(4).
APA Wang, Jing.,Liu, Huaide.,Zhang, Xuan.,Li, Xinpeng.,Geng, Lihua.,...&Zhang, Quanbin.(2017).Sulfated Hetero-Polysaccharides Protect SH-SY5Y Cells from H2O2-Induced Apoptosis by Affecting the PI3K/Akt Signaling Pathway.MARINE DRUGS,15(4).
MLA Wang, Jing,et al."Sulfated Hetero-Polysaccharides Protect SH-SY5Y Cells from H2O2-Induced Apoptosis by Affecting the PI3K/Akt Signaling Pathway".MARINE DRUGS 15.4(2017).
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