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The effect of different substitute groups and molecular weights of fucoidan on neuroprotective and anticomplement activity 期刊论文
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2018, 卷号: 113, 页码: 82-89
Authors:  Liu, Huaide;  Wang, Jing;  Zhang, Quanbin;  Zhang, Hong
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Fucoidan derivatives  Molecular weights  Substitute group  Neuroprotective  Anticomplement  
H-pylori alpha 1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides 期刊论文
CHEMICAL COMMUNICATIONS, 2017, 卷号: 53, 期号: 80, 页码: 11012-11015
Authors:  Yu, Hai;  Li, Yanhong;  Wu, Zhigang;  Li, Lei;  Zeng, Jie;  Zhao, Chao;  Wu, Yijing;  Tasnima, Nova;  Wang, Jing;  Liu, Huaide;  Gadi, Madhusudhan Reddy;  Guan, Wanyi;  Wang, Peng G.;  Chen, Xi
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Sulfated Hetero-Polysaccharides Protect SH-SY5Y Cells from H2O2-Induced Apoptosis by Affecting the PI3K/Akt Signaling Pathway 期刊论文
MARINE DRUGS, 2017, 卷号: 15, 期号: 4
Authors:  Wang, Jing;  Liu, Huaide;  Zhang, Xuan;  Li, Xinpeng;  Geng, Lihua;  Zhang, Hong;  Zhang, Quanbin
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Sulfated Hetero-polysaccharides  Pi3k/akt  Phosphorylate  Sh-sy5y  Oxidative Stress  
Structure-activity relationship of sulfated hetero/galactofucan polysaccharides on dopaminergic neuron 期刊论文
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2016, 卷号: 82, 期号: 2016, 页码: 878-883
Authors:  Wang, Jing;  Liu, Huaide;  Jin, Weihua;  Zhang, Hong;  Zhang, Quanbin
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Sulfated Hetero-polysaccharides  Sulfated Galactofucan Polysaccharides  Neuron Protection  
The Protective Effect of Fucoidan in Rats with Streptozotocin-Induced Diabetic Nephropathy 期刊论文
MARINE DRUGS, 2014, 卷号: 12, 期号: 6, 页码: 3292-3306
Authors:  Wang, Jing;  Liu, Huaide;  Li, Ning;  Zhang, Quanbin;  Zhang, Hong;  Li, N (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
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Sulfated Polysaccharide  Fucoidan  Diabetic Nephropathy  
The intestinal microbial diversity in Chinese shrimp (Fenneropenaeus chinensis) as determined by PCR-DGGE and clone library analyses 期刊论文
AQUACULTURE, 2011, 卷号: 317, 期号: 1-4, 页码: 32-36
Authors:  Liu, Huaide;  Wang, Lei;  Liu, Mei;  Wang, Baojie;  Jiang, Keyong;  Ma, Shaosai;  Li, Qiufen
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Intestinal Microbial Diversity  Pcr-dgge  16s Rdna Clone Library  Chinese Shrimp  
PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus) 期刊论文
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY, 2010, 卷号: 28, 期号: 4, 页码: 808-814
Authors:  Liu Huaide;  Liu Mei;  Wang Baojie;  Jiang Keyong;  Jiang Shan;  Sun Shujuan;  Wang Lei
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Intestinal Microbial Diversity  Pcr-dgge  Kuruma Shrimp  
一种对虾肠道微生物DNA的提取方法 专利
专利类型: 发明, 专利号: CN200910263665.0, 申请日期: 2010-06-23, 公开日期: 2010-06-23
Inventors:  刘淮德;  王雷;  刘梅;  王宝杰;  蒋克勇
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一种对虾肠道微生物dna的提取方法  其特征在于：1)将0.01-0.05g对虾肠道在液氮下研磨  2)将洗脱液在200g离心5-10min  并加入0.8-1.2ml?pbs、15-25μl?pvpp匀浆和200-300μl?0.5％tween-80  取上清菌液待用  沉淀中加0.8-1.2ml?pbs以200g离心取上清菌液  3)将步骤2)所得上清菌液以1200g离心5-10min  均匀后吹打洗脱3-5min  将两次离心所得上清菌液合并  收集上清菌液  合并上清液以300g离心5-10min  4)将步骤3)所得上清菌液中加100-150μlctab提取液和10-15μl溶菌酶  取上清菌液待用  放入37℃摇床  而后加15-20μl?20％(W/c)Sds和10-15μl蛋白酶k  振荡30-40min  60-70℃水浴1-2h  5)将水浴后菌液在4℃?3200g离心8-15min  取上清菌液  6)将水浴后菌液以3200g离心8-15min  而后加100-150μl?ctab液和20-25μl?20％sds混匀后  取上清菌液  7)将步骤6)所得上清菌液中加上清菌液体积0.6-0.8倍的异丙醇  涡旋  而后加入与上述上清菌液等体积的酚/氯仿混合液  而后于-20℃静置1-2h  8)将步骤7)所得沉淀中加入600-800μl?70％预冷乙醇  60-70℃水浴10-15min  14000g离心10-15min  再以4℃?14000g离心10-15min  14000g离心10-15min  取上清菌液待用  所得沉淀待用  所得沉淀即为对虾肠道微生物dna。  
应用微生物分子生态学方法研究对虾肠道细菌组成及其变化规律 学位论文
, 北京: 中国科学院研究生院, 2010
Authors:  刘淮德
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对虾  Pcr-dgge 法  16s Rdna 克隆文库法  肠道微生物多样性  
中国明对虾（Fenneropenaeus chinensis）肠道微生物区系组成的分子分析 期刊论文
饲料工业, 2010, 卷号: 31, 期号: 4, 页码: 55-57
Authors:  刘淮德;  刘梅;  王宝杰;  蒋克勇;  张国范;  王雷
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