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中国科学院海洋研究所机构知识库
Knowledge Management System Of Institute of Oceanology, Chinese Academy of Sciences
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海洋生物技术研发中心 [9]
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The effect of different substitute groups and molecular weights of fucoidan on neuroprotective and anticomplement activity
期刊论文
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2018, 卷号: 113, 页码: 82-89
Authors:
Liu, Huaide
;
Wang, Jing
;
Zhang, Quanbin
;
Zhang, Hong
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Submit date:2019/08/21
Fucoidan derivatives
Molecular weights
Substitute group
Neuroprotective
Anticomplement
H-pylori alpha 1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides
期刊论文
CHEMICAL COMMUNICATIONS, 2017, 卷号: 53, 期号: 80, 页码: 11012-11015
Authors:
Yu, Hai
;
Li, Yanhong
;
Wu, Zhigang
;
Li, Lei
;
Zeng, Jie
;
Zhao, Chao
;
Wu, Yijing
;
Tasnima, Nova
;
Wang, Jing
;
Liu, Huaide
;
Gadi, Madhusudhan Reddy
;
Guan, Wanyi
;
Wang, Peng G.
;
Chen, Xi
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Submit date:2018/01/11
Sulfated Hetero-Polysaccharides Protect SH-SY5Y Cells from H2O2-Induced Apoptosis by Affecting the PI3K/Akt Signaling Pathway
期刊论文
MARINE DRUGS, 2017, 卷号: 15, 期号: 4
Authors:
Wang, Jing
;
Liu, Huaide
;
Zhang, Xuan
;
Li, Xinpeng
;
Geng, Lihua
;
Zhang, Hong
;
Zhang, Quanbin
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Submit date:2017/10/09
Sulfated Hetero-polysaccharides
Pi3k/akt
Phosphorylate
Sh-sy5y
Oxidative Stress
Structure-activity relationship of sulfated hetero/galactofucan polysaccharides on dopaminergic neuron
期刊论文
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2016, 卷号: 82, 期号: 2016, 页码: 878-883
Authors:
Wang, Jing
;
Liu, Huaide
;
Jin, Weihua
;
Zhang, Hong
;
Zhang, Quanbin
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Submit date:2016/02/05
Sulfated Hetero-polysaccharides
Sulfated Galactofucan Polysaccharides
Neuron Protection
The Protective Effect of Fucoidan in Rats with Streptozotocin-Induced Diabetic Nephropathy
期刊论文
MARINE DRUGS, 2014, 卷号: 12, 期号: 6, 页码: 3292-3306
Authors:
Wang, Jing
;
Liu, Huaide
;
Li, Ning
;
Zhang, Quanbin
;
Zhang, Hong
;
Li, N (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
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Submit date:2015/06/11
Sulfated Polysaccharide
Fucoidan
Diabetic Nephropathy
The intestinal microbial diversity in Chinese shrimp (Fenneropenaeus chinensis) as determined by PCR-DGGE and clone library analyses
期刊论文
AQUACULTURE, 2011, 卷号: 317, 期号: 1-4, 页码: 32-36
Authors:
Liu, Huaide
;
Wang, Lei
;
Liu, Mei
;
Wang, Baojie
;
Jiang, Keyong
;
Ma, Shaosai
;
Li, Qiufen
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Submit date:2012/07/03
Intestinal Microbial Diversity
Pcr-dgge
16s Rdna Clone Library
Chinese Shrimp
PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus)
期刊论文
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY, 2010, 卷号: 28, 期号: 4, 页码: 808-814
Authors:
Liu Huaide
;
Liu Mei
;
Wang Baojie
;
Jiang Keyong
;
Jiang Shan
;
Sun Shujuan
;
Wang Lei
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Submit date:2010/12/24
Intestinal Microbial Diversity
Pcr-dgge
Kuruma Shrimp
一种对虾肠道微生物DNA的提取方法
专利
专利类型: 发明, 专利号: CN200910263665.0, 申请日期: 2010-06-23, 公开日期: 2010-06-23
Inventors:
刘淮德
;
王雷
;
刘梅
;
王宝杰
;
蒋克勇
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Submit date:2014/08/04
一种对虾肠道微生物dna的提取方法
其特征在于:1)将0.01-0.05g对虾肠道在液氮下研磨
2)将洗脱液在200g离心5-10min
并加入0.8-1.2ml?pbs、15-25μl?pvpp匀浆和200-300μl?0.5%tween-80
取上清菌液待用
沉淀中加0.8-1.2ml?pbs以200g离心取上清菌液
3)将步骤2)所得上清菌液以1200g离心5-10min
均匀后吹打洗脱3-5min
将两次离心所得上清菌液合并
收集上清菌液
合并上清液以300g离心5-10min
4)将步骤3)所得上清菌液中加100-150μlctab提取液和10-15μl溶菌酶
取上清菌液待用
放入37℃摇床
而后加15-20μl?20%(W/c)Sds和10-15μl蛋白酶k
振荡30-40min
60-70℃水浴1-2h
5)将水浴后菌液在4℃?3200g离心8-15min
取上清菌液
6)将水浴后菌液以3200g离心8-15min
而后加100-150μl?ctab液和20-25μl?20%sds混匀后
取上清菌液
7)将步骤6)所得上清菌液中加上清菌液体积0.6-0.8倍的异丙醇
涡旋
而后加入与上述上清菌液等体积的酚/氯仿混合液
而后于-20℃静置1-2h
8)将步骤7)所得沉淀中加入600-800μl?70%预冷乙醇
60-70℃水浴10-15min
14000g离心10-15min
再以4℃?14000g离心10-15min
14000g离心10-15min
取上清菌液待用
所得沉淀待用
所得沉淀即为对虾肠道微生物dna。
应用微生物分子生态学方法研究对虾肠道细菌组成及其变化规律
学位论文
, 北京: 中国科学院研究生院, 2010
Authors:
刘淮德
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Submit date:2012/06/20
对虾
Pcr-dgge 法
16s Rdna 克隆文库法
肠道微生物多样性
中国明对虾(Fenneropenaeus chinensis)肠道微生物区系组成的分子分析
期刊论文
饲料工业, 2010, 卷号: 31, 期号: 4, 页码: 55-57
Authors:
刘淮德
;
刘梅
;
王宝杰
;
蒋克勇
;
张国范
;
王雷
Adobe PDF(274Kb)
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Submit date:2011/07/25