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Formation of resting cells is accompanied with enrichment of ferritin in marine diatom Phaeodactylum tricornutum
Liu, Xuehua1,2,3,4; Wang, Lijun1,2,3; Wu, Songcui1,2,3; Zhou, Lu1,2,3,4; Gao, Shan1,2,3; Xie, Xiujun1,2,3; Wang, Lepu1,2,3,4; Gu, Wenhui1,2,3; Wang, Guangce1,2,3
2022
Source PublicationALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
Volume61Pages:10
Corresponding AuthorGu, Wenhui(wenhuigu@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
AbstractThe formation of resting cells/spores in marine diatoms helps them to survive in environmental changes, whose sedimentation characteristics also drive enhanced carbon fluxes export to the deep ocean. However, the molecular mechanism underlying the transformation remains unclear. Based on the upregulation of ferritin during dormancy in microorganism and its' crucial role in coping with environmental stress, we constructed the ferritin overexpression and silence strains in model marine diatom, Phaeodactylum tricornutum (FTN-OE and FTN-Si, respectively). The morphological transformation from fusiform to ovoid were observed among the FTN-OE strains but not the wide type and FTN-Si strains. These FTN-OE oval cells exhibited typical characteristics of resting cells and benthic adaptation, such as lower growth rates, low-light adaptation, silicified valves, the excretion of exopolymeric substances, upregulation of stress-resistant proteins and increased nitrate reserves. Furthermore, FTN-OE oval cells showed increased abscisic acid synthesis and signaling pathways in the transcriptome and proteome. And the abscisic acid signal was detected in FTN-OE oval cells exclusively (2.96 ng g(-1) FW), but absent in the FTN-Si, FTN-OE fusiform or WT cells. Overall, our results demonstrate that ferritin overexpression in marine diatom have a close relationship with morphological transformation from fusiform to ovoid morphotype, after which they enter a resting stage, which is accompanied by abscisic acid accumulation. This improves our understanding of the molecular processes in diatom resting cells/spores' formation and suggests that ferritin accumulation might improve the sequestration of photosynthetically fixed atmospheric CO2 into the deep sea.
KeywordFerritin Morphological change Oval Abscisic acid Dormancy
DOI10.1016/j.algal.2021.102567
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFD0901500] ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences[COMS2019Q02] ; Shandong Provincial RD Program[2018GHY115019] ; Chinese Academy of Sciences[KLEMB-DYS02] ; National Natural Science Foundation of China[41676157] ; National Natural Science Foundation of China[41976097] ; National Natural Science Foundation of China[41806171] ; National Natural Science Foundation of China[41876158] ; Haikou Marine Economic Demonstration City Industrial Chain Collaborative Innovation Project[HHCL201811] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000730984400005
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/177540
Collection实验海洋生物学重点实验室
Corresponding AuthorGu, Wenhui; Wang, Guangce
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS, Qingdao, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
4.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Xuehua,Wang, Lijun,Wu, Songcui,et al. Formation of resting cells is accompanied with enrichment of ferritin in marine diatom Phaeodactylum tricornutum[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2022,61:10.
APA Liu, Xuehua.,Wang, Lijun.,Wu, Songcui.,Zhou, Lu.,Gao, Shan.,...&Wang, Guangce.(2022).Formation of resting cells is accompanied with enrichment of ferritin in marine diatom Phaeodactylum tricornutum.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,61,10.
MLA Liu, Xuehua,et al."Formation of resting cells is accompanied with enrichment of ferritin in marine diatom Phaeodactylum tricornutum".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 61(2022):10.
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