Formation of resting cells is accompanied with enrichment of ferritin in marine diatom Phaeodactylum tricornutum

doi:10.1016/j.algal.2021.102567

IOCAS-IR > 实验海洋生物学重点实验室

	Formation of resting cells is accompanied with enrichment of ferritin in marine diatom Phaeodactylum tricornutum
	Liu, Xuehua 1,2,3,4; Wang, Lijun 1,2,3; Wu, Songcui1,2,3 ; Zhou, Lu 1,2,3,4; Gao, Shan 1,2,3; Xie, Xiujun 1,2,3; Wang, Lepu 1,2,3,4; Gu, Wenhui 1,2,3; Wang, Guangce 1,2,3
	2022
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN	2211-9264
卷号	61 页码:10
通讯作者	Gu, Wenhui(wenhuigu@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
摘要	The formation of resting cells/spores in marine diatoms helps them to survive in environmental changes, whose sedimentation characteristics also drive enhanced carbon fluxes export to the deep ocean. However, the molecular mechanism underlying the transformation remains unclear. Based on the upregulation of ferritin during dormancy in microorganism and its' crucial role in coping with environmental stress, we constructed the ferritin overexpression and silence strains in model marine diatom, Phaeodactylum tricornutum (FTN-OE and FTN-Si, respectively). The morphological transformation from fusiform to ovoid were observed among the FTN-OE strains but not the wide type and FTN-Si strains. These FTN-OE oval cells exhibited typical characteristics of resting cells and benthic adaptation, such as lower growth rates, low-light adaptation, silicified valves, the excretion of exopolymeric substances, upregulation of stress-resistant proteins and increased nitrate reserves. Furthermore, FTN-OE oval cells showed increased abscisic acid synthesis and signaling pathways in the transcriptome and proteome. And the abscisic acid signal was detected in FTN-OE oval cells exclusively (2.96 ng g(-1) FW), but absent in the FTN-Si, FTN-OE fusiform or WT cells. Overall, our results demonstrate that ferritin overexpression in marine diatom have a close relationship with morphological transformation from fusiform to ovoid morphotype, after which they enter a resting stage, which is accompanied by abscisic acid accumulation. This improves our understanding of the molecular processes in diatom resting cells/spores' formation and suggests that ferritin accumulation might improve the sequestration of photosynthetically fixed atmospheric CO2 into the deep sea.
关键词	Ferritin Morphological change Oval Abscisic acid Dormancy
DOI	10.1016/j.algal.2021.102567
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0901500] ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences[COMS2019Q02] ; Shandong Provincial RD Program[2018GHY115019] ; Chinese Academy of Sciences[KLEMB-DYS02] ; National Natural Science Foundation of China[41676157] ; National Natural Science Foundation of China[41976097] ; National Natural Science Foundation of China[41806171] ; National Natural Science Foundation of China[41876158] ; Haikou Marine Economic Demonstration City Industrial Chain Collaborative Innovation Project[HHCL201811] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000730984400005
出版者	ELSEVIER
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177540
专题	实验海洋生物学重点实验室
通讯作者	Gu, Wenhui; Wang, Guangce
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Liu, Xuehua,Wang, Lijun,Wu, Songcui,et al. Formation of resting cells is accompanied with enrichment of ferritin in marine diatom Phaeodactylum tricornutum[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2022,61:10.
APA	Liu, Xuehua.,Wang, Lijun.,Wu, Songcui.,Zhou, Lu.,Gao, Shan.,...&Wang, Guangce.(2022).Formation of resting cells is accompanied with enrichment of ferritin in marine diatom Phaeodactylum tricornutum.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,61,10.
MLA	Liu, Xuehua,et al."Formation of resting cells is accompanied with enrichment of ferritin in marine diatom Phaeodactylum tricornutum".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 61(2022):10.

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