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Acetylome profiling of Vibrio alginolyticus reveals its role in bacterial virulence
Pang, Huanying1,2,3,4,7,8; Li, Wanxin6; Zhang, Weijie2,3,7; Zhou, Shihui2,3,7; Hoare, Rowena5; Monaghan, Sean J.5; Jian, Jichang2,3,4,7,8; Lin, Xiangmin6
2020-01-16
Source PublicationJOURNAL OF PROTEOMICS
ISSN1874-3919
Volume211Pages:9
Corresponding AuthorJian, Jichang(jianjic@gmail.com) ; Lin, Xiangmin(xiangmin@fafu.edu.cn)
AbstractIt is well known that lysine acetylation (Kace) modification is a common post-translational modification (PTM) that plays an important role in multiple biological and pathological functions in bacteria. However, few studies have focused on lysine acetylation modification in aquatic pathogens to date. In this study, the acetylome profiling of fish pathogen, Vibrio alginolyticus was investigated by combining affinity enrichment with LC MS/ MS. A total of 2883 acetylation modification sites on 1178 proteins in this pathogen were identified. The Kace modification of several selected proteins were further validated by Co-immunocoprecipitation combined with Western blotting. Bioinformatics analysis showed that seven conserved motifs can be enriched among Kace peptides, and many of them were significantly enriched in metabolic processes such as biosynthesis of secondary metabolites, microbial metabolism in diverse environments, and biosynthesis of amino acids, which was similar to data previously published for V. parahaemolyticus. Moreover, we found at least 102 acetylation modified proteins predicted as virulence factors, which indicate the important role of PTM on bacterial virulence. In general, our results provide a promising starting point for further investigations of the biological role of lysine acetylation on bacterial virulence in V. alginolyticus. Biological significance: Lysine acetylation (Kace) modification, is well known to play important roles on diverse biological functions in prokaryotic cell, whereas few studies focused on aquatic pathogens to date. In this study, the acetylome profiling of fish pathogen, Vibrio alginolyticus was investigated by combining affinity enrichment with LC MS/MS. A total of 2883 acetylation modification sites on 1178 proteins in this pathogen were identified. The further bioinformatics analysis showed that seven conserved motifs can be enriched among Kace peptides, and many of them were significantly enriched in metabolic processes, which was similar to data previously published for V. parahemolyticus. Moreover, we found at least 102 acetylation modified proteins predicted as virulence factors, which indicate the important role of PTM on bacterial virulence. In general, our results provide a promising starting point for further investigations of the biological role of lysine acetylation on bacterial virulence in V. alginolyticus.
KeywordVibrio alginolyticus Acetylome Bacterial virulence Proteomics
DOI10.1016/j.jprot.2019.103543
Indexed BySCI
Language英语
Funding ProjectShenzhen Science and Technology Project[JCYJ20170818111629778] ; National Key Research and Development Program of China[2018YFD0900501] ; National Natural Science Foundation of China[31402344] ; National Natural Science Foundation of China[31670129] ; Natural Science Foundation of Guangdong Province[2017A030313174] ; Program for Innovative Research Team in Fujian Agricultural and Forestry University[712018009] ; Innovation and Entrepreneurship Training Program for College Students[CXXL2019041]
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000500375300005
PublisherELSEVIER
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163802
Collection实验海洋生物学重点实验室
Corresponding AuthorJian, Jichang; Lin, Xiangmin
Affiliation1.Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 510000, Guangdong, Peoples R China
2.Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524025, Peoples R China
3.Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524025, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
5.Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland
6.Fujian Agr & Forestry Univ, Sch Life Sci, Fujian Prov Key Lab Agroecol Proc & Safety Monito, Fuzhou, Fujian, Peoples R China
7.Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang 524025, Peoples R China
8.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Pang, Huanying,Li, Wanxin,Zhang, Weijie,et al. Acetylome profiling of Vibrio alginolyticus reveals its role in bacterial virulence[J]. JOURNAL OF PROTEOMICS,2020,211:9.
APA Pang, Huanying.,Li, Wanxin.,Zhang, Weijie.,Zhou, Shihui.,Hoare, Rowena.,...&Lin, Xiangmin.(2020).Acetylome profiling of Vibrio alginolyticus reveals its role in bacterial virulence.JOURNAL OF PROTEOMICS,211,9.
MLA Pang, Huanying,et al."Acetylome profiling of Vibrio alginolyticus reveals its role in bacterial virulence".JOURNAL OF PROTEOMICS 211(2020):9.
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