Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Acetylome profiling of Vibrio alginolyticus reveals its role in bacterial virulence | |
Pang, Huanying1,2,3,4,7,8; Li, Wanxin6; Zhang, Weijie2,3,7; Zhou, Shihui2,3,7; Hoare, Rowena5; Monaghan, Sean J.5; Jian, Jichang2,3,4,7,8; Lin, Xiangmin6 | |
2020-01-16 | |
发表期刊 | JOURNAL OF PROTEOMICS |
ISSN | 1874-3919 |
卷号 | 211页码:9 |
通讯作者 | Jian, Jichang(jianjic@gmail.com) ; Lin, Xiangmin(xiangmin@fafu.edu.cn) |
摘要 | It is well known that lysine acetylation (Kace) modification is a common post-translational modification (PTM) that plays an important role in multiple biological and pathological functions in bacteria. However, few studies have focused on lysine acetylation modification in aquatic pathogens to date. In this study, the acetylome profiling of fish pathogen, Vibrio alginolyticus was investigated by combining affinity enrichment with LC MS/ MS. A total of 2883 acetylation modification sites on 1178 proteins in this pathogen were identified. The Kace modification of several selected proteins were further validated by Co-immunocoprecipitation combined with Western blotting. Bioinformatics analysis showed that seven conserved motifs can be enriched among Kace peptides, and many of them were significantly enriched in metabolic processes such as biosynthesis of secondary metabolites, microbial metabolism in diverse environments, and biosynthesis of amino acids, which was similar to data previously published for V. parahaemolyticus. Moreover, we found at least 102 acetylation modified proteins predicted as virulence factors, which indicate the important role of PTM on bacterial virulence. In general, our results provide a promising starting point for further investigations of the biological role of lysine acetylation on bacterial virulence in V. alginolyticus. Biological significance: Lysine acetylation (Kace) modification, is well known to play important roles on diverse biological functions in prokaryotic cell, whereas few studies focused on aquatic pathogens to date. In this study, the acetylome profiling of fish pathogen, Vibrio alginolyticus was investigated by combining affinity enrichment with LC MS/MS. A total of 2883 acetylation modification sites on 1178 proteins in this pathogen were identified. The further bioinformatics analysis showed that seven conserved motifs can be enriched among Kace peptides, and many of them were significantly enriched in metabolic processes, which was similar to data previously published for V. parahemolyticus. Moreover, we found at least 102 acetylation modified proteins predicted as virulence factors, which indicate the important role of PTM on bacterial virulence. In general, our results provide a promising starting point for further investigations of the biological role of lysine acetylation on bacterial virulence in V. alginolyticus. |
关键词 | Vibrio alginolyticus Acetylome Bacterial virulence Proteomics |
DOI | 10.1016/j.jprot.2019.103543 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Shenzhen Science and Technology Project[JCYJ20170818111629778] ; National Key Research and Development Program of China[2018YFD0900501] ; National Natural Science Foundation of China[31402344] ; National Natural Science Foundation of China[31670129] ; Natural Science Foundation of Guangdong Province[2017A030313174] ; Program for Innovative Research Team in Fujian Agricultural and Forestry University[712018009] ; Innovation and Entrepreneurship Training Program for College Students[CXXL2019041] ; Shenzhen Science and Technology Project[JCYJ20170818111629778] ; National Key Research and Development Program of China[2018YFD0900501] ; National Natural Science Foundation of China[31402344] ; National Natural Science Foundation of China[31670129] ; Natural Science Foundation of Guangdong Province[2017A030313174] ; Program for Innovative Research Team in Fujian Agricultural and Forestry University[712018009] ; Innovation and Entrepreneurship Training Program for College Students[CXXL2019041] |
WOS研究方向 | Biochemistry & Molecular Biology |
WOS类目 | Biochemical Research Methods |
WOS记录号 | WOS:000500375300005 |
出版者 | ELSEVIER |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/163802 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Jian, Jichang; Lin, Xiangmin |
作者单位 | 1.Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 510000, Guangdong, Peoples R China 2.Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524025, Peoples R China 3.Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524025, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 5.Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland 6.Fujian Agr & Forestry Univ, Sch Life Sci, Fujian Prov Key Lab Agroecol Proc & Safety Monito, Fuzhou, Fujian, Peoples R China 7.Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang 524025, Peoples R China 8.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Pang, Huanying,Li, Wanxin,Zhang, Weijie,et al. Acetylome profiling of Vibrio alginolyticus reveals its role in bacterial virulence[J]. JOURNAL OF PROTEOMICS,2020,211:9. |
APA | Pang, Huanying.,Li, Wanxin.,Zhang, Weijie.,Zhou, Shihui.,Hoare, Rowena.,...&Lin, Xiangmin.(2020).Acetylome profiling of Vibrio alginolyticus reveals its role in bacterial virulence.JOURNAL OF PROTEOMICS,211,9. |
MLA | Pang, Huanying,et al."Acetylome profiling of Vibrio alginolyticus reveals its role in bacterial virulence".JOURNAL OF PROTEOMICS 211(2020):9. |
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