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Sargassum henslowianum as a potential biofilter in mariculture farms of a subtropical eutrophic bay 期刊论文
MARINE POLLUTION BULLETIN, 2019, 卷号: 149, 页码: 10
Authors:  Yu, Zonghe;  Sun, Hongyan;  Huang, Wen;  Hu, Chaoqun;  Zhou, Yi
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Aquaculture pollution  Seaweed  Biofilter  Bioaccumulation  Nutrients  Heavy metals  
Reference gene selection for microRNA expression analysis in the Pacific oyster 期刊论文
ROMANIAN BIOTECHNOLOGICAL LETTERS, 2018, 卷号: 23, 期号: 5, 页码: 14043-14050
Authors:  Huang, Wen;  Xu, Fei;  Wang, Wei;  Li, Li;  Que, Huayong;  Zhang, Guofan
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Crassostrea gigas  MicroRNA  Real-time quantitative PCR  Reference gene  
Identification of Thyroid Hormones and Functional Characterization of Thyroid Hormone Receptor in the Pacific Oyster Crassostrea gigas Provide Insight into Evolution of the Thyroid Hormone System 期刊论文
PLOS ONE, 2015, 卷号: 10, 期号: 12, 页码: 0144991
Authors:  Huang, Wen;  Xu, Fei;  Qu, Tao;  Zhang, Rui;  Li, Li;  Que, Huayong;  Zhang, Guofan
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Cloning and characterization of a novel caspase-8-like gene in Crassostrea gigas 期刊论文
FISH & SHELLFISH IMMUNOLOGY, 2015, 卷号: 46, 期号: 2, 页码: 486-492
Authors:  Li, Chunyan;  Qu, Tao;  Huang, Baoyu;  Ji, Peng;  Huang, Wen;  Que, Huayong;  Li, Li;  Zhang, Guofan
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Crassostrea Gigas  Cgcaspase8-2  Apoptosis  Immune Response  
Evolution of a novel nuclear receptor subfamily with emphasis on the member from the Pacific oyster Crassostrea gigas 期刊论文
GENE, 2015, 卷号: 567, 期号: 2, 页码: 164-172
Authors:  Huang, Wen;  Xu, Fei;  Li, Juan;  Li, Li;  Que, Huayong;  Zhang, Guofan
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Nuclear Hormone Receptor  Transcription Factor  Nr8 Subfamily  Dna Binding  Clone  Metazoan  
Iodothyronine deiodinase gene analysis of the Pacific oyster Crassostrea gigas reveals possible conservation of thyroid hormone feedback regulation mechanism in mollusks 期刊论文
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY, 2015, 卷号: 33, 期号: 4, 页码: 997-1006
Authors:  Huang Wen;  Xu Fei;  Qu Tao;  Li Li;  Que Huayong;  Zhang Guofan
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Iodothyronine Deiodinase  Crassostrea Gigas  Thyroid Hormone (Th)  Clone  Expression Analysis  
长牡蛎甲状腺激素及其信号通路关键基因的功能研究 学位论文
, 北京: 中国科学院大学, 2015
Authors:  黄雯
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长牡蛎  甲状腺激素  核受体  
Identification of Conserved and Novel MicroRNAs in the Pacific Oyster Crassostrea gigas by Deep Sequencing 期刊论文
PLOS ONE, 2014, 卷号: 9, 期号: 8, 页码: e104371
Authors:  Xu, Fei;  Wang, Xiaotong;  Feng, Yue;  Huang, Wen;  Wang, Wei;  Li, Li;  Fang, Xiaodong;  Que, Huayong;  Zhang, Guofan;  Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Peoples R China.
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Identification and Functional Characterization of Two Executioner Caspases in Crassostrea gigas 期刊论文
PLOS ONE, 2014, 卷号: 9, 期号: 2, 页码: e89040
Authors:  Qu, Tao;  Huang, Baoyu;  Zhang, Linlin;  Li, Li;  Xu, Fei;  Huang, Wen;  Li, Chunyan;  Du, Yishuai;  Zhang, Guofan;  Li, L (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China.
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一种在贝类幼虫中定量检测甲状腺激素的方法 专利
专利类型: 发明, 专利号: CN201210545897.7, 申请日期: 2014-01-22, 公开日期: 2014-01-22
Inventors:  黄雯;  阙华勇;  许飞;  李莉;  张国范
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一种在贝类幼虫中定量检测甲状腺激素的方法  其特征在于:该方法包括如下步骤:1)将幼虫样品用液氮研磨法研磨成粉末状  2)研磨后进行低温干燥  分成ab两部分分别用于以下步骤  3)将a部分样品准确称量干重后  按固定比例加入0.01mol/l?naoh溶液  4)8000?12000rpm离心5?10min  2?8℃下充分浸润1h以上提取甲状腺激素  取上清  5)取步骤4)中的上清  用于定量检测甲状腺激素的含量  即总甲状腺素t4和总3  7)将步骤6)中裂解细胞释放蛋白用于检测样品中总蛋白含量  5  6)将b部分样品准确称量干重后加入细胞裂解液和pmsf室温放置1h以上  3′?三碘甲腺原氨酸t3  裂解细胞释放蛋白  8)将数据整合  计算出幼虫中每克蛋白含甲状腺激素x克  即xg/gprotein。