Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Characterization of two types of vascular endothelial growth factor from Litopenaeus vannamei and their involvements during WSSV infection | |
Wang, Zhiwei1,2 ; Li, Shihao1,3; Li, Fuhua1,3; Yang, Hui1,2; Yang, Fusheng4; Xiang, Jianhai1
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| 2015-12-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| 卷号 | 47期号:2页码:824-832 |
| 文章类型 | Article |
| 摘要 | Vascular endothelial growth factors (VEGFs) are important signaling proteins in VEGF signaling pathway which play key roles in inducing endothelial cell proliferation, migration, angiogenesis, vascular permeability, inhibition of apoptosis and virus infection. In the present study, we isolated and characterized two VEGF genes, LvVEGF1 and LvVEGF2 from Litopenaeus vannamei. The deduced amino acid sequences of both LvVEGF1 and LvVEGF2 contained a signal peptide, a typical PDGF/VEGF domain and a cysteine knot motif (CXCXC). Tissue distribution analysis showed that LvVEGF1 was predominantly expressed in lymphoid organ (Oka) while LvVEGF2 was mainly detected in gill and hemocytes. The transcriptional levels of LvVEGF1 in Oka and LvVEGF2 in gill or hemocytes were apparently up-regulated during WSSV infection. Double-stranded RNA interference was used for further functional studies. The data showed that silencing of LvVEGF1 and LvVEGF2 caused a decrease of the copy numbers of the virus in WSSV infected shrimp and a reduction of the cumulative mortality rate of shrimp during WSSV infection. The present study indicated that LvVEGF1 and LvVEGF2 might facilitate WSSV infection, which provided new evidence to understand the function of VEGF signaling pathway during WSSV infection in shrimp. (C) 2015 Elsevier Ltd. All rights reserved. |
| 关键词 | Vascular Endothelial Growth Factor Litopenaeus Vannamei Wssv Rnai Accumulated Mortality |
| DOI | 10.1016/j.fsi.2015.10.026 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000366874500023 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/86634 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 4.Hangzhou Xiaoshan Donghai Aquaculture Co Ltd, Hangzhou 311200, Zhejiang, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Zhiwei,Li, Shihao,Li, Fuhua,et al. Characterization of two types of vascular endothelial growth factor from Litopenaeus vannamei and their involvements during WSSV infection[J]. FISH & SHELLFISH IMMUNOLOGY,2015,47(2):824-832. |
| APA | Wang, Zhiwei,Li, Shihao,Li, Fuhua,Yang, Hui,Yang, Fusheng,&Xiang, Jianhai.(2015).Characterization of two types of vascular endothelial growth factor from Litopenaeus vannamei and their involvements during WSSV infection.FISH & SHELLFISH IMMUNOLOGY,47(2),824-832. |
| MLA | Wang, Zhiwei,et al."Characterization of two types of vascular endothelial growth factor from Litopenaeus vannamei and their involvements during WSSV infection".FISH & SHELLFISH IMMUNOLOGY 47.2(2015):824-832. |
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