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Characterization of two types of vascular endothelial growth factor from Litopenaeus vannamei and their involvements during WSSV infection
Wang, Zhiwei1,2; Li, Shihao1,3; Li, Fuhua1,3; Yang, Hui1,2; Yang, Fusheng4; Xiang, Jianhai1
2015-12-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume47Issue:2Pages:824-832
SubtypeArticle
AbstractVascular endothelial growth factors (VEGFs) are important signaling proteins in VEGF signaling pathway which play key roles in inducing endothelial cell proliferation, migration, angiogenesis, vascular permeability, inhibition of apoptosis and virus infection. In the present study, we isolated and characterized two VEGF genes, LvVEGF1 and LvVEGF2 from Litopenaeus vannamei. The deduced amino acid sequences of both LvVEGF1 and LvVEGF2 contained a signal peptide, a typical PDGF/VEGF domain and a cysteine knot motif (CXCXC). Tissue distribution analysis showed that LvVEGF1 was predominantly expressed in lymphoid organ (Oka) while LvVEGF2 was mainly detected in gill and hemocytes. The transcriptional levels of LvVEGF1 in Oka and LvVEGF2 in gill or hemocytes were apparently up-regulated during WSSV infection. Double-stranded RNA interference was used for further functional studies. The data showed that silencing of LvVEGF1 and LvVEGF2 caused a decrease of the copy numbers of the virus in WSSV infected shrimp and a reduction of the cumulative mortality rate of shrimp during WSSV infection. The present study indicated that LvVEGF1 and LvVEGF2 might facilitate WSSV infection, which provided new evidence to understand the function of VEGF signaling pathway during WSSV infection in shrimp. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordVascular Endothelial Growth Factor Litopenaeus Vannamei Wssv Rnai Accumulated Mortality
DOI10.1016/j.fsi.2015.10.026
Indexed BySCI
Language英语
WOS IDWOS:000366874500023
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/86634
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
4.Hangzhou Xiaoshan Donghai Aquaculture Co Ltd, Hangzhou 311200, Zhejiang, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
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GB/T 7714
Wang, Zhiwei,Li, Shihao,Li, Fuhua,et al. Characterization of two types of vascular endothelial growth factor from Litopenaeus vannamei and their involvements during WSSV infection[J]. FISH & SHELLFISH IMMUNOLOGY,2015,47(2):824-832.
APA Wang, Zhiwei,Li, Shihao,Li, Fuhua,Yang, Hui,Yang, Fusheng,&Xiang, Jianhai.(2015).Characterization of two types of vascular endothelial growth factor from Litopenaeus vannamei and their involvements during WSSV infection.FISH & SHELLFISH IMMUNOLOGY,47(2),824-832.
MLA Wang, Zhiwei,et al."Characterization of two types of vascular endothelial growth factor from Litopenaeus vannamei and their involvements during WSSV infection".FISH & SHELLFISH IMMUNOLOGY 47.2(2015):824-832.
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