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Identification, mRNA expression profiling and activity characterization of cathepsin L from red drum (Sciaenops ocellatus)
Sun, Bo-guang; Hu, Yong-hua
2015-12-01
发表期刊FISH PHYSIOLOGY AND BIOCHEMISTRY
卷号41期号:6页码:1463-1473
文章类型Article
摘要Cathepsin L is a cysteine protease with a papain-like structure. It is known to be implicated in multiple processes of mammalian immune response to pathogen infection. In teleost fish, the functionality of cathepsin L is less understood. In this work, we characterized a cathepsin L homologue (designated as SoCatL) from red drum Sciaenops ocellatus, an important farmed fish species in China. SoCatL possesses a typical domain arrangement characteristic of cathepsin L, which comprises a proregion and a protease domain with four catalytically essential residues (Gln137, Cys143, His282 and Asn302) conserved in various organisms. SoCatL shares moderate sequence identities with mammalian cathepsin L and relatively high sequence identities with teleost cathepsin L. Phylogenetic analysis revealed that SoCatL is evolutionally close to fish cathepsin L, especially those belonging to the Perciformes order. The homology model of SoCatL was discovered to exhibit a structure resembling human cathepsin L. Transcriptional expression of SoCatL was found ubiquitous in tissues and enhanced after experimental infection with a bacterial pathogen. Recombinant SoCatL purified from Escherichia coli (designated as rSoCatL) displayed apparent proteolytic activity, which was optimal at 50 A degrees C and pH 7.0. The activity of rSoCatL required the catalytic residue Cys143 and was severely reduced by cathepsin inhibitor. These results suggest that SoCatL is a teleost cathepsin L homologue which functions as a cysteine protease and is likely to participate in the host immune response against bacterial infection.
关键词Cathepsin l Sciaenops Ocellatus Teleost Fish Red Drum
DOI10.1007/s10695-015-0099-9
收录类别SCI
语种英语
WOS记录号WOS:000364517600009
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/82293
专题实验海洋生物学重点实验室
作者单位Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
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Sun, Bo-guang,Hu, Yong-hua. Identification, mRNA expression profiling and activity characterization of cathepsin L from red drum (Sciaenops ocellatus)[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2015,41(6):1463-1473.
APA Sun, Bo-guang,&Hu, Yong-hua.(2015).Identification, mRNA expression profiling and activity characterization of cathepsin L from red drum (Sciaenops ocellatus).FISH PHYSIOLOGY AND BIOCHEMISTRY,41(6),1463-1473.
MLA Sun, Bo-guang,et al."Identification, mRNA expression profiling and activity characterization of cathepsin L from red drum (Sciaenops ocellatus)".FISH PHYSIOLOGY AND BIOCHEMISTRY 41.6(2015):1463-1473.
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