IOCAS-IR  > 海洋环境腐蚀与与生物污损重点实验室
Lysozyme as a recognition element for monitoring of bacterial population
Zheng, Laibao1; Wan, Yi2; Yu, Liangmin1; Zhang, Dun2
2016
Source PublicationTALANTA
Volume146Issue:2015Pages:299-302
SubtypeArticle
AbstractBacterial infections remain a significant challenge in biomedicine and environment safety. Increasing worldwide demand for point-of-care techniques and increasing concern on their safe development and use, require a simple and sensitive bioanalysis for pathogen detection. However, this goal is not yet achieved. A design for fluorescein isothiocyanate-labeled lysozyme (FITC-LYZ), which provides quantitative binding information for gram-positive bacteria, Micrococcus luteus, and detects pathogen concentration, is presented. The functional lysozyme is used not only as the pathogenic detection platform, but also as a tracking reagent for microbial population in antibacterial tests. A nonlinear relationship between the system response and the logarithm of the bacterial concentration was observed in the range of 1.2 x 10(2)-1.2 x 10(5) cfu mL(-1). The system has a potential for further applications and provides a facile and simple method for detection of pathogenic bacteria. Meanwhile, the fluorescein isothiocyanate-labeled lysozyme is also employed as the tracking agent for antibacterial dynamic assay, which show a similar dynamic curve compared with UV-vis test. (C) 2015 Elsevier B.V. All rights reserved.
KeywordLysozyme Recognition Element Bacterial Population Bacterial Detection
DOI10.1016/j.talanta.2015.08.056
Indexed BySCI
Language英语
WOS IDWOS:000363815600042
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/82288
Collection海洋环境腐蚀与与生物污损重点实验室
Affiliation1.Ocean Univ China, Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Zheng, Laibao,Wan, Yi,Yu, Liangmin,et al. Lysozyme as a recognition element for monitoring of bacterial population[J]. TALANTA,2016,146(2015):299-302.
APA Zheng, Laibao,Wan, Yi,Yu, Liangmin,&Zhang, Dun.(2016).Lysozyme as a recognition element for monitoring of bacterial population.TALANTA,146(2015),299-302.
MLA Zheng, Laibao,et al."Lysozyme as a recognition element for monitoring of bacterial population".TALANTA 146.2015(2016):299-302.
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