Lysozyme as a recognition element for monitoring of bacterial population

doi:10.1016/j.talanta.2015.08.056

	Lysozyme as a recognition element for monitoring of bacterial population
	Zheng, Laibao1 ; Wan, Yi 2; Yu, Liangmin 1; Zhang, Dun 2
	2016
发表期刊	TALANTA
卷号	146 期号:2015 页码:299-302
文章类型	Article
摘要	Bacterial infections remain a significant challenge in biomedicine and environment safety. Increasing worldwide demand for point-of-care techniques and increasing concern on their safe development and use, require a simple and sensitive bioanalysis for pathogen detection. However, this goal is not yet achieved. A design for fluorescein isothiocyanate-labeled lysozyme (FITC-LYZ), which provides quantitative binding information for gram-positive bacteria, Micrococcus luteus, and detects pathogen concentration, is presented. The functional lysozyme is used not only as the pathogenic detection platform, but also as a tracking reagent for microbial population in antibacterial tests. A nonlinear relationship between the system response and the logarithm of the bacterial concentration was observed in the range of 1.2 x 10(2)-1.2 x 10(5) cfu mL(-1). The system has a potential for further applications and provides a facile and simple method for detection of pathogenic bacteria. Meanwhile, the fluorescein isothiocyanate-labeled lysozyme is also employed as the tracking agent for antibacterial dynamic assay, which show a similar dynamic curve compared with UV-vis test. (C) 2015 Elsevier B.V. All rights reserved.
关键词	Lysozyme Recognition Element Bacterial Population Bacterial Detection
DOI	10.1016/j.talanta.2015.08.056
收录类别	SCI
语种	英语
WOS记录号	WOS:000363815600042
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/82288
专题	海洋环境腐蚀与生物污损重点实验室
作者单位	1.Ocean Univ China, Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Laibao,Wan, Yi,Yu, Liangmin,et al. Lysozyme as a recognition element for monitoring of bacterial population[J]. TALANTA,2016,146(2015):299-302.
APA	Zheng, Laibao,Wan, Yi,Yu, Liangmin,&Zhang, Dun.(2016).Lysozyme as a recognition element for monitoring of bacterial population.TALANTA,146(2015),299-302.
MLA	Zheng, Laibao,et al."Lysozyme as a recognition element for monitoring of bacterial population".TALANTA 146.2015(2016):299-302.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Lysozyme as a recogn（960KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览