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Empty pericarp7 encodes a mitochondrial E-subgroup pentatricopeptide repeat protein that is required for ccmF(N) editing, mitochondrial function and seed development in maize
Sun, Feng1; Wang, Xiaomin2; Bonnard, Geraldine3; Shen, Yun4; Xiu, Zhihui4; Li, Xiaojie4; Gao, Dahai5; Zhang, Zhonghang4; Tan, Bao-Cai1
2015-10-01
Source PublicationPLANT JOURNAL
Volume84Issue:2Pages:283-295
SubtypeArticle
AbstractRNA editing, converting cytidines (C) to uridines (U) at specific sites in the transcripts of mitochondria and plastids, plays a critical role in organelle gene expression in land plants. Recently pentatricopeptide repeat (PPR) proteins were identified as site-specific recognition factors for RNA editing. In this study, we characterized an empty pericarp7 mutant (emp7) in Zea mays (maize), which confers an embryo-lethal phenotype. In emp7 mutants, mitochondrial functions are seriously perturbed, resulting in a strikingly reduced respiration rate. Emp7 encodes an E-subgroup PPR protein that is localized exclusively in the mitochondrion. Null mutation of Emp7 abolishes the CU editing of ccmF(N) transcript solely at position 1553. CcmF(N) is coding for a subunit of heme lyase complex in the cytochromec maturation pathway. The resulting PheSer substitution in CcmF(N) leads to the loss of CcmF(N) protein and a strikingly reduced c-type cytochrome. Consequently, the mitochondrial cytochrome-linked respiratory chain is impaired as a result of the disassembly of complexIII in the emp7 mutant. These results indicate that the PPR-E subgroup protein EMP7 is required for CU editing of ccmF(N)-1553 at a position essential for cytochromec maturation and mitochondrial oxidative phosphorylation, and hence is essential to embryo and endosperm development in maize.
KeywordMaize Empty Pericarp7 Cytochromec Maturation Mitochondrion Seed Development
DOI10.1111/tpj.12993
Indexed BySCI
Language英语
WOS IDWOS:000362692000003
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/52152
Collection实验海洋生物学重点实验室
Affiliation1.Shandong Univ, Sch Life Sci, Key Lab Plant Cell Engn & Germplasm Innovat, Minist Educ, Jinan 250100, Peoples R China
2.Lanzhou Univ, Sch Life Sci, Key Lab Cell Act & Stress Adaptat, Minist Educ, Lanzhou 730000, Peoples R China
3.Associe Univ Strasbourg, Inst Biol Mol Plantes CNRS, F-67084 Strasbourg, France
4.Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Hong Kong, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
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GB/T 7714
Sun, Feng,Wang, Xiaomin,Bonnard, Geraldine,et al. Empty pericarp7 encodes a mitochondrial E-subgroup pentatricopeptide repeat protein that is required for ccmF(N) editing, mitochondrial function and seed development in maize[J]. PLANT JOURNAL,2015,84(2):283-295.
APA Sun, Feng.,Wang, Xiaomin.,Bonnard, Geraldine.,Shen, Yun.,Xiu, Zhihui.,...&Tan, Bao-Cai.(2015).Empty pericarp7 encodes a mitochondrial E-subgroup pentatricopeptide repeat protein that is required for ccmF(N) editing, mitochondrial function and seed development in maize.PLANT JOURNAL,84(2),283-295.
MLA Sun, Feng,et al."Empty pericarp7 encodes a mitochondrial E-subgroup pentatricopeptide repeat protein that is required for ccmF(N) editing, mitochondrial function and seed development in maize".PLANT JOURNAL 84.2(2015):283-295.
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