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Maternally derived trypsin may have multiple functions in the early development of turbot (Scopthalmus maximus)
Chi, Liang; Liu, Qinghua; Xu, Shihong; Xiao, Zhizhong; Ma, Daoyuan; Li, Jun
2015-10-01
发表期刊COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
卷号188期号:2015页码:148-155
文章类型Article
摘要Trypsin is an important serine protease that is considered to be involved in digestion of protein in teleost fish. Nevertheless, studies on trypsin/trypsinogen in fish embryos are very limited. In this study, the trypsinogen of turbot (Scophthalmus maximus) (tTG) was identified and the expression patterns and activity of trypsinogen/trypsin were investigated. The results showed that the tTG mRNA was evenly distributed in the oocytes and was also expressed along the yolk periphery in early embryos. At later embryo stages-and 1 days after hatching (dph), the tTG mRNA concentrated at the alimentary tract and head. Quantitative expression analysis showed that the tTG transcripts decreased after fertilization until the gastrula stage, then increased with the embryo and larvae development This result was also confirmed by the specific activity analysis of trypsin and in-situ-hybridization (ISH). All of the results indicated that tTG in early embryo stages was maternally derived and expressed by itself after gastrula stages. Additionally, location of tTG mRNA in embryos and larvae was investigated; we considered that trypsin may have multiple functions during the embryo development process. Based on our results regarding trypsinogen in embryos and early development, we concluded that the trypsin/trypsinogen in turbot embryos was inherited from a maternal source and we suggested that trypsin in early development has multiple functions in the process of development. (C) 2015 Elsevier Inc. All rights reserved.
关键词Turbot (Scophthalmus Maximus) Trypsinogen/trypsin Maternal Development
DOI10.1016/j.cbpa.2015.06.034
收录类别SCI
语种英语
WOS记录号WOS:000360513400018
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/50426
专题海洋生物技术研发中心
作者单位Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China
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Chi, Liang,Liu, Qinghua,Xu, Shihong,et al. Maternally derived trypsin may have multiple functions in the early development of turbot (Scopthalmus maximus)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,2015,188(2015):148-155.
APA Chi, Liang,Liu, Qinghua,Xu, Shihong,Xiao, Zhizhong,Ma, Daoyuan,&Li, Jun.(2015).Maternally derived trypsin may have multiple functions in the early development of turbot (Scopthalmus maximus).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,188(2015),148-155.
MLA Chi, Liang,et al."Maternally derived trypsin may have multiple functions in the early development of turbot (Scopthalmus maximus)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 188.2015(2015):148-155.
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