IOCAS-IR  > 海洋生物技术研发中心
Maternally derived trypsin may have multiple functions in the early development of turbot (Scopthalmus maximus)
Chi, Liang; Liu, Qinghua; Xu, Shihong; Xiao, Zhizhong; Ma, Daoyuan; Li, Jun
2015-10-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
Volume188Issue:2015Pages:148-155
SubtypeArticle
AbstractTrypsin is an important serine protease that is considered to be involved in digestion of protein in teleost fish. Nevertheless, studies on trypsin/trypsinogen in fish embryos are very limited. In this study, the trypsinogen of turbot (Scophthalmus maximus) (tTG) was identified and the expression patterns and activity of trypsinogen/trypsin were investigated. The results showed that the tTG mRNA was evenly distributed in the oocytes and was also expressed along the yolk periphery in early embryos. At later embryo stages-and 1 days after hatching (dph), the tTG mRNA concentrated at the alimentary tract and head. Quantitative expression analysis showed that the tTG transcripts decreased after fertilization until the gastrula stage, then increased with the embryo and larvae development This result was also confirmed by the specific activity analysis of trypsin and in-situ-hybridization (ISH). All of the results indicated that tTG in early embryo stages was maternally derived and expressed by itself after gastrula stages. Additionally, location of tTG mRNA in embryos and larvae was investigated; we considered that trypsin may have multiple functions during the embryo development process. Based on our results regarding trypsinogen in embryos and early development, we concluded that the trypsin/trypsinogen in turbot embryos was inherited from a maternal source and we suggested that trypsin in early development has multiple functions in the process of development. (C) 2015 Elsevier Inc. All rights reserved.
KeywordTurbot (Scophthalmus Maximus) Trypsinogen/trypsin Maternal Development
DOI10.1016/j.cbpa.2015.06.034
Indexed BySCI
Language英语
WOS IDWOS:000360513400018
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/50426
Collection海洋生物技术研发中心
AffiliationChinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Chi, Liang,Liu, Qinghua,Xu, Shihong,et al. Maternally derived trypsin may have multiple functions in the early development of turbot (Scopthalmus maximus)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,2015,188(2015):148-155.
APA Chi, Liang,Liu, Qinghua,Xu, Shihong,Xiao, Zhizhong,Ma, Daoyuan,&Li, Jun.(2015).Maternally derived trypsin may have multiple functions in the early development of turbot (Scopthalmus maximus).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,188(2015),148-155.
MLA Chi, Liang,et al."Maternally derived trypsin may have multiple functions in the early development of turbot (Scopthalmus maximus)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 188.2015(2015):148-155.
Files in This Item:
File Name/Size DocType Version Access License
Maternally derived t(1104KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chi, Liang]'s Articles
[Liu, Qinghua]'s Articles
[Xu, Shihong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chi, Liang]'s Articles
[Liu, Qinghua]'s Articles
[Xu, Shihong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chi, Liang]'s Articles
[Liu, Qinghua]'s Articles
[Xu, Shihong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Maternally derived trypsin may have multiple functions in the early development of turbot (Scopthalmus maximus).pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.