IOCAS-IR  > 实验海洋生物学重点实验室
An integrin from oyster Crassostrea gigas mediates the phagocytosis toward Vibrio splendidus through LPS binding activity
Jia, Zhihao1,4; Zhang, Tao1,4; Jiang, Shuai1; Wang, Mengqiang1; Cheng, Qi3; Sun, Mingzhe1,4; Wang, Lingling1; Song, Linsheng2
2015-11-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume53Issue:1Pages:253-264
SubtypeArticle
AbstractIntegrins are a family of cell adhesion molecules which play important roles in the regulation of cell adhesion, migration, proliferation, apoptosis and phagocytosis. In the present study, the immune function of an integrin from the oyster Crassostrea gigas (designated CgIntegrin) was characterized to understand the regulatory mechanism of hemocyte phagocytosis toward different microbes. The full-length cDNA of CgIntegrin was 2571 bp with an open reading frame (ORF) of 2397 bp, encoding a polypeptide of 799 amino acids. The mRNA transcripts of CgIntegrin were predominantly detected in hemocytes, gonad and adductor muscle, while lowly in hepatopancreas, mantle and gill. The mRNA expression level was up-regulated at 6 h post lipopolysaccharide (LPS) stimulation (p < 0.01), while no significant change was observed after peptidoglycan (PGN) stimulation. The oyster hemocytes with relative high CgIntegrin expression level exhibited different phagocytic abilities towards different microorganism and particles, such as Gram-positive bacteria Vibrio splendidus, Gram-negative bacteria Staphylococcus aureus and latex beads. Moreover, the phagocytic rate towards V. splendidus was significantly decreased after the blockade of CgIntegrin using the polyclonal antibody. The recombinant CgIntegrin (rCgIntegrin) displayed agglutinating activity towards V splendidus but not S. aureus and Y. lipolytica. It also exhibited a higher binding affinity towards LPS (compared to rTrx group) in a dose-dependent manner with the apparent dissociation constant (K-d) of 5.53 x 10(-6) M. The results indicated that CgIntegrin served as a pattern recognition receptor with LPS binding activity, which could directly bind to V. splendidus and enhance the phagocytosis of oyster hemocytes. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordCrassostrea Gigas Innate Immunity Integrin Phagocytosis Liposaccharides Vibrio Splendidus
DOI10.1016/j.dci.2015.07.014
Indexed BySCI
Language英语
WOS IDWOS:000360772900027
Citation statistics
Cited Times:37[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/50420
Collection实验海洋生物学重点实验室
海洋生态与环境科学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
3.Dalian Polytech Univ, Dalian 116034, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jia, Zhihao,Zhang, Tao,Jiang, Shuai,et al. An integrin from oyster Crassostrea gigas mediates the phagocytosis toward Vibrio splendidus through LPS binding activity[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,53(1):253-264.
APA Jia, Zhihao.,Zhang, Tao.,Jiang, Shuai.,Wang, Mengqiang.,Cheng, Qi.,...&Song, Linsheng.(2015).An integrin from oyster Crassostrea gigas mediates the phagocytosis toward Vibrio splendidus through LPS binding activity.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,53(1),253-264.
MLA Jia, Zhihao,et al."An integrin from oyster Crassostrea gigas mediates the phagocytosis toward Vibrio splendidus through LPS binding activity".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 53.1(2015):253-264.
Files in This Item:
File Name/Size DocType Version Access License
An integrin from oys(5047KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jia, Zhihao]'s Articles
[Zhang, Tao]'s Articles
[Jiang, Shuai]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jia, Zhihao]'s Articles
[Zhang, Tao]'s Articles
[Jiang, Shuai]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jia, Zhihao]'s Articles
[Zhang, Tao]'s Articles
[Jiang, Shuai]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: An integrin from oyster Crassostrea gigas mediates the phagocytosis toward Vibrio.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.