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The sperm proteome of the Pacific oyster Crassostrea gigas and immunolocalization of heat shock proteins
Huang, Xiaohong1,2; Liu, Baozhong1; Huan, Pin1
2015-07-03
Source PublicationINVERTEBRATE REPRODUCTION & DEVELOPMENT
Volume59Issue:3Pages:111-118
SubtypeArticle
AbstractThe Pacific oyster Crassostrea gigas is a potential model organism of bivalve mollusks. Comprehensive studies on the proteome of its sperm are necessary to expand our understanding on its reproduction and development, which however are still poor currently. In this study, to improve the situation, we conducted a proteomic analysis on the sperm based on two-dimensional electrophoresis combined with protein identification through mass spectra data. Fifty-six protein spots with constant and relatively high expression levels were selected for protein identification. Among them, 36 were identified (corresponding to 31 proteins), including cytoskeletal proteins, proteins involved in energy supply, protein modifiers, signal regulators, antioxidant proteins, and others. We proposed that these proteins might play important roles in sperm motility, gamete interaction, and oxidation resistance. In particular, we observed several heat shock proteins that were proved to play essential roles in animal sperms. A further immunofluorescence experiment revealed a mitochondria localization of Hsp60s and wide distributions of Hsp70s, indicating these proteins might function in various processes such as mitochondrial function, gamete interaction, and regulation of receptor activity. Our data will provide fundamental supports for the studies on the mechanisms of fertilization and contribute to expand the understandings on reproduction and development of bivalve mollusks.
KeywordGamete 2-de Bivalve Fertilization
DOI10.1080/07924259.2015.1041654
Indexed BySCI
Language英语
WOS IDWOS:000359740100001
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/49946
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Huang, Xiaohong,Liu, Baozhong,Huan, Pin. The sperm proteome of the Pacific oyster Crassostrea gigas and immunolocalization of heat shock proteins[J]. INVERTEBRATE REPRODUCTION & DEVELOPMENT,2015,59(3):111-118.
APA Huang, Xiaohong,Liu, Baozhong,&Huan, Pin.(2015).The sperm proteome of the Pacific oyster Crassostrea gigas and immunolocalization of heat shock proteins.INVERTEBRATE REPRODUCTION & DEVELOPMENT,59(3),111-118.
MLA Huang, Xiaohong,et al."The sperm proteome of the Pacific oyster Crassostrea gigas and immunolocalization of heat shock proteins".INVERTEBRATE REPRODUCTION & DEVELOPMENT 59.3(2015):111-118.
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