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A new pattern of primordial germ cell migration in olive flounder (Paralichthys olivaceus) identified using nanos3
Li, Meijie1,2; Tan, Xungang1; Jiao, Shuang1; Wang, Qian1; Wu, Zhihao1; You, Feng1; Zou, Yuxia1
2015-07-01
发表期刊DEVELOPMENT GENES AND EVOLUTION
卷号225期号:4页码:195-206
文章类型Article
摘要The olive flounder (Paralichthys olivaceus) is an important cultured marine fish. However, little information is available on primordial germ cell (PGC) development and migration in this species; such information is vital for applications in artificial reproduction and for the preservation of genetic resources. Here, we sought to remedy this information deficit by isolating the germline-specific gene nanos3 and analyzing its expression in olive flounder. Sequencing analysis showed that olive flounder nanos3 contained a typical RNA-binding zinc finger domain. A phylogenetic analysis demonstrated that nanos3 of the olive flounder grouped with that of the barfin flounder (Verasper moseri). In the olive flounder, nanos3 was consistently expressed during embryogenesis. Whole-mount in situ hybridization showed that a new pattern of PGC migration was present in olive flounder, which combined elements of the PGC migration patterns of medaka, herring, and goby. In olive flounder, PGCs aligned along the lateral plate mesoderm in two loose, elongated lines at early embryogenesis, aggregated into a single loose cluster at mid-embryogenesis, then re-aligned into two tight clusters at late somitogenesis, and finally migrated to the genital ridge as two clusters. Furthermore, whole-mount in situ hybridization revealed that expression of stromal derived factor 1 (Sdf1) was important for guiding of PGC migration during somitogenesis. In particular, Sdf1 directed aggregation of PGCs into a single loose cluster from the two elongated lines during mid-embryogenesis. Additionally, PGCs in zebrafish were successfully visualized by injection of chimeric RNA containing the green fluorescent protein (GFP) and 3' untranslated region of olive flounder nanos3. These findings provide new insights into PGC migration and development in olive flounder and will also facilitate germ cell manipulation in this species.
关键词Teleost Fish Pgc Migration Nanos3 Sdf1 Expression Pattern
DOI10.1007/s00427-015-0503-6
收录类别SCI
语种英语
WOS记录号WOS:000358876800001
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/49613
专题实验海洋生物学重点实验室
海洋地质与环境重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Li, Meijie,Tan, Xungang,Jiao, Shuang,et al. A new pattern of primordial germ cell migration in olive flounder (Paralichthys olivaceus) identified using nanos3[J]. DEVELOPMENT GENES AND EVOLUTION,2015,225(4):195-206.
APA Li, Meijie.,Tan, Xungang.,Jiao, Shuang.,Wang, Qian.,Wu, Zhihao.,...&Zou, Yuxia.(2015).A new pattern of primordial germ cell migration in olive flounder (Paralichthys olivaceus) identified using nanos3.DEVELOPMENT GENES AND EVOLUTION,225(4),195-206.
MLA Li, Meijie,et al."A new pattern of primordial germ cell migration in olive flounder (Paralichthys olivaceus) identified using nanos3".DEVELOPMENT GENES AND EVOLUTION 225.4(2015):195-206.
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