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CpG ODNs induced autophagy via reactive oxygen species (ROS) in Chinese mitten crab, Eriocheir sinensis
Sun, Mingzhe1,2; Wang, Lingling1; Jiang, Shuai1; Liu, Rui1; Zhao, Depeng3; Chen, Hao1,2; Song, Xiaorui1,2; Song, Linsheng4
2015-09-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume52Issue:1Pages:1-9
SubtypeArticle
AbstractAutophagy is a highly conserved intracellular homeostatic process involved in numerous responses in both vertebrate and invertebrate. In the present study, autophagy in hemocytes of Chinese mitten crab Eriocheir sinensis was observed by Western-blot and immunofluorescence assay, and its induction by CpG oligodeoxynucleotides (ODNs) was investigated. The increase of LC3-conversion (LC3-II/LC3-I) and LC3-puncta formation were observed in hemocytes of crabs after rapamycin injection. And the ratio of LC3-conversion and the percentage of LC3-puncta formation were also significantly increased after CpG ODNs stimulation, and the highest values were 1.89-fold and 3.77-fold compared to that in pUC57 group at 24 h post-injection. Moreover, the mRNA expression levels of autophagy-related genes, EsGabarap and EsAtg7, both dramatically increased after CpG ODNs injection, and reached the peak at 6 h post-injection, which were 2.66- and 2.82-fold (P < 0.01) for EsGabarap, and 6.16-fold and 6.10-fold (P < 0.01) for EsAtg7 compared to saline and pUC57 groups, respectively. The generation of ROS in hemocytes was induced and reached peak at 6 h post-injection in CpG-pUC57 group, which was 1.30-fold (P < 0.01) and 1.66-fold (P < 0.01) of that in saline and pUC57 group, respectively. The increased ROS generation and autophagy triggered by CpG ODNs were abolished after the treatment of the ROS scavenger, N-acetyl-L-cysteine (NAC). It was suggested that CpG ODNs could induce autophagy and up-regulate the expression levels of autophagy-related genes in crabs via the activation of ROS generation in the hemocytes. The results provided useful information to understand autophagy in crab, and they were also helpful for the application of CpG ODNs as the novel immune stimulants in aquaculture. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordCpg Odns Autophagy Ros Eriocheir Sinensis Innate Immune Response
DOI10.1016/j.dci.2015.04.008
Indexed BySCI
Language英语
WOS IDWOS:000357145900001
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/48993
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Polytech Univ, Dalian 116034, Peoples R China
4.Dalian Ocean Univ, Dalian 116023, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
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Sun, Mingzhe,Wang, Lingling,Jiang, Shuai,et al. CpG ODNs induced autophagy via reactive oxygen species (ROS) in Chinese mitten crab, Eriocheir sinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,52(1):1-9.
APA Sun, Mingzhe.,Wang, Lingling.,Jiang, Shuai.,Liu, Rui.,Zhao, Depeng.,...&Song, Linsheng.(2015).CpG ODNs induced autophagy via reactive oxygen species (ROS) in Chinese mitten crab, Eriocheir sinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,52(1),1-9.
MLA Sun, Mingzhe,et al."CpG ODNs induced autophagy via reactive oxygen species (ROS) in Chinese mitten crab, Eriocheir sinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 52.1(2015):1-9.
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