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Polymerase chain reaction method to detect canis materials by amplification of species-specific DNA fragment
Gao, HW; Xu, BL; Liang, CZ; Zhang, YB; Zhu, LH
2004-09-01
发表期刊JOURNAL OF AOAC INTERNATIONAL
卷号87期号:5页码:1195-1199
文章类型Article
摘要Rapid identification of mammal materials in feeding stuffs and food is essential for effective control of a potential source of pathogens, such as those that cause bovine spongiform encephalopathy. A convenient polymerase chain reaction (PCR)-based assay was developed for detection and identification of a canis-specific mitochondrial DNA sequence in foodstuffs and food. The amplified canis-specific PCR product was a 213 base pair band from the D-loop DNA fragment of mitochondria, a high copy gene which should improve the possibility of amplifying template molecules of adequate size among the degraded DNA fragments brought about by heat denaturation. The specificity of this method was confirmed by 8 canis blood DNA samples (from different breeds of dog) and 9 noncanis animal blood DNA samples (bovine, sheep, porcine, chicken, fish, donkey, rabbit, deer, horse). This method was able to detect the presence of canis material in foodstuffs and in food mixtures even when the concentration of canis-derived meat was reduced to 0.05%. Furthermore, it did not appear to be affected by prolonged heat treatment. This method was developed for detection of canis materials in feeding stuffs, and occasionally for medical jurisprudence detection of canis-derived materials.
收录类别SCI
语种英语
WOS记录号WOS:000223867100023
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/33009
专题实验海洋生物学重点实验室
作者单位1.Qindao Entry Exit Inspect & Quarantine Bur, Food Lab, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
3.China Import & Export Commod Inspect Technol Inst, Beijing, Peoples R China
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GB/T 7714
Gao, HW,Xu, BL,Liang, CZ,et al. Polymerase chain reaction method to detect canis materials by amplification of species-specific DNA fragment[J]. JOURNAL OF AOAC INTERNATIONAL,2004,87(5):1195-1199.
APA Gao, HW,Xu, BL,Liang, CZ,Zhang, YB,&Zhu, LH.(2004).Polymerase chain reaction method to detect canis materials by amplification of species-specific DNA fragment.JOURNAL OF AOAC INTERNATIONAL,87(5),1195-1199.
MLA Gao, HW,et al."Polymerase chain reaction method to detect canis materials by amplification of species-specific DNA fragment".JOURNAL OF AOAC INTERNATIONAL 87.5(2004):1195-1199.
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