IOCAS-IR  > 实验海洋生物学重点实验室
Polymerase chain reaction method to detect canis materials by amplification of species-specific DNA fragment
Gao, HW; Xu, BL; Liang, CZ; Zhang, YB; Zhu, LH
2004-09-01
Source PublicationJOURNAL OF AOAC INTERNATIONAL
Volume87Issue:5Pages:1195-1199
SubtypeArticle
AbstractRapid identification of mammal materials in feeding stuffs and food is essential for effective control of a potential source of pathogens, such as those that cause bovine spongiform encephalopathy. A convenient polymerase chain reaction (PCR)-based assay was developed for detection and identification of a canis-specific mitochondrial DNA sequence in foodstuffs and food. The amplified canis-specific PCR product was a 213 base pair band from the D-loop DNA fragment of mitochondria, a high copy gene which should improve the possibility of amplifying template molecules of adequate size among the degraded DNA fragments brought about by heat denaturation. The specificity of this method was confirmed by 8 canis blood DNA samples (from different breeds of dog) and 9 noncanis animal blood DNA samples (bovine, sheep, porcine, chicken, fish, donkey, rabbit, deer, horse). This method was able to detect the presence of canis material in foodstuffs and in food mixtures even when the concentration of canis-derived meat was reduced to 0.05%. Furthermore, it did not appear to be affected by prolonged heat treatment. This method was developed for detection of canis materials in feeding stuffs, and occasionally for medical jurisprudence detection of canis-derived materials.
Indexed BySCI
Language英语
WOS IDWOS:000223867100023
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/33009
Collection实验海洋生物学重点实验室
Affiliation1.Qindao Entry Exit Inspect & Quarantine Bur, Food Lab, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
3.China Import & Export Commod Inspect Technol Inst, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Gao, HW,Xu, BL,Liang, CZ,et al. Polymerase chain reaction method to detect canis materials by amplification of species-specific DNA fragment[J]. JOURNAL OF AOAC INTERNATIONAL,2004,87(5):1195-1199.
APA Gao, HW,Xu, BL,Liang, CZ,Zhang, YB,&Zhu, LH.(2004).Polymerase chain reaction method to detect canis materials by amplification of species-specific DNA fragment.JOURNAL OF AOAC INTERNATIONAL,87(5),1195-1199.
MLA Gao, HW,et al."Polymerase chain reaction method to detect canis materials by amplification of species-specific DNA fragment".JOURNAL OF AOAC INTERNATIONAL 87.5(2004):1195-1199.
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