Polymerase chain reaction method to detect canis materials by amplification of species-specific DNA fragment

IOCAS-IR > 实验海洋生物学重点实验室

	Polymerase chain reaction method to detect canis materials by amplification of species-specific DNA fragment
	Gao, HW ; Xu, BL ; Liang, CZ ; Zhang, YB ; Zhu, LH
	2004-09-01
发表期刊	JOURNAL OF AOAC INTERNATIONAL
卷号	87 期号:5 页码:1195-1199
文章类型	Article
摘要	Rapid identification of mammal materials in feeding stuffs and food is essential for effective control of a potential source of pathogens, such as those that cause bovine spongiform encephalopathy. A convenient polymerase chain reaction (PCR)-based assay was developed for detection and identification of a canis-specific mitochondrial DNA sequence in foodstuffs and food. The amplified canis-specific PCR product was a 213 base pair band from the D-loop DNA fragment of mitochondria, a high copy gene which should improve the possibility of amplifying template molecules of adequate size among the degraded DNA fragments brought about by heat denaturation. The specificity of this method was confirmed by 8 canis blood DNA samples (from different breeds of dog) and 9 noncanis animal blood DNA samples (bovine, sheep, porcine, chicken, fish, donkey, rabbit, deer, horse). This method was able to detect the presence of canis material in foodstuffs and in food mixtures even when the concentration of canis-derived meat was reduced to 0.05%. Furthermore, it did not appear to be affected by prolonged heat treatment. This method was developed for detection of canis materials in feeding stuffs, and occasionally for medical jurisprudence detection of canis-derived materials.
收录类别	SCI
语种	英语
WOS记录号	WOS:000223867100023
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/33009
专题	实验海洋生物学重点实验室
作者单位	1.Qindao Entry Exit Inspect & Quarantine Bur, Food Lab, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 3.China Import & Export Commod Inspect Technol Inst, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Gao, HW,Xu, BL,Liang, CZ,et al. Polymerase chain reaction method to detect canis materials by amplification of species-specific DNA fragment[J]. JOURNAL OF AOAC INTERNATIONAL,2004,87(5):1195-1199.
APA	Gao, HW,Xu, BL,Liang, CZ,Zhang, YB,&Zhu, LH.(2004).Polymerase chain reaction method to detect canis materials by amplification of species-specific DNA fragment.JOURNAL OF AOAC INTERNATIONAL,87(5),1195-1199.
MLA	Gao, HW,et al."Polymerase chain reaction method to detect canis materials by amplification of species-specific DNA fragment".JOURNAL OF AOAC INTERNATIONAL 87.5(2004):1195-1199.

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