A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity

doi:10.5897/AJB11.2017

IOCAS-IR > 实验海洋生物学重点实验室

	A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity
	Wang, Leilei 1,2; Zhang, Ying 1,2; Wang, Lingling 1; Yang, Jialong 1,2; Zhou, Zhi 1,2; Gai, Yunchao 1,2; Qiu, Limei 1; Song, Linsheng 1
	2011-12-05
发表期刊	AFRICAN JOURNAL OF BIOTECHNOLOGY
卷号	10 期号:77 页码:17678-17689
文章类型	Article
摘要	The cDNA of a new Eriocheir sinensis ALF (designated as EsALF-3) was obtained based on EST analysis. The full-length cDNA was of 956 bp, consisting of an open reading frame (ORF) of 369 bp encoding a polypeptide of 123 amino acids. In the deduced amino acid sequence of EsALF-3, there were two highly conserved cysteine residues to define the LPS binding site, and eight positively charged residues existed between the two cysteine residues. The phylogenetic analysis revealed that EsALF-3 was clustered with the ALFs from some crustaceans, forming a separate sister branch to the group of EsALF-1 and EsALF-2 from E. sinensis. The mRNA transcript of EsALF-3 was detected in all the examined tissues of crabs, including haemocytes, hepatopancreas, gill, muscle, heart and gonad. The recombinant protein of EsALF-3 represented antimicrobial activity against Listonella anguillarum, Escherichia coli and Bacillus subtilis with minimal inhibitory concentration (MIC) values of 33.75 mu g mL(-1), 270 mu g mL-1 and 135 mu g mL(-1), respectively. These results together indicated that there were multiplicate ALF isoforms coexist in E. sinensis, and the diversity of the immune effector molecules in innate immune system, such as ALF, helps Chinese mitten crab to deal with various pathogens in the aquatic environment.
关键词	Eriocheir Sinensis Anti-lipopolysaccharide Factor Listonella Anguillarum Bacillus Subtilis Antimicrobial Activity
DOI	10.5897/AJB11.2017
收录类别	SCI
语种	英语
WOS记录号	WOS:000298556200004
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32942
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wang, Leilei,Zhang, Ying,Wang, Lingling,et al. A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(77):17678-17689.
APA	Wang, Leilei.,Zhang, Ying.,Wang, Lingling.,Yang, Jialong.,Zhou, Zhi.,...&Song, Linsheng.(2011).A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(77),17678-17689.
MLA	Wang, Leilei,et al."A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.77(2011):17678-17689.

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文件名称/大小	文献类型	版本类型	开放类型	使用许可
A new anti-lipopolys（650KB）	期刊论文	出版稿	限制开放	ODC PDDL	浏览