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A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity
Wang, Leilei1,2; Zhang, Ying1,2; Wang, Lingling1; Yang, Jialong1,2; Zhou, Zhi1,2; Gai, Yunchao1,2; Qiu, Limei1; Song, Linsheng1
2011-12-05
Source PublicationAFRICAN JOURNAL OF BIOTECHNOLOGY
Volume10Issue:77Pages:17678-17689
SubtypeArticle
AbstractThe cDNA of a new Eriocheir sinensis ALF (designated as EsALF-3) was obtained based on EST analysis. The full-length cDNA was of 956 bp, consisting of an open reading frame (ORF) of 369 bp encoding a polypeptide of 123 amino acids. In the deduced amino acid sequence of EsALF-3, there were two highly conserved cysteine residues to define the LPS binding site, and eight positively charged residues existed between the two cysteine residues. The phylogenetic analysis revealed that EsALF-3 was clustered with the ALFs from some crustaceans, forming a separate sister branch to the group of EsALF-1 and EsALF-2 from E. sinensis. The mRNA transcript of EsALF-3 was detected in all the examined tissues of crabs, including haemocytes, hepatopancreas, gill, muscle, heart and gonad. The recombinant protein of EsALF-3 represented antimicrobial activity against Listonella anguillarum, Escherichia coli and Bacillus subtilis with minimal inhibitory concentration (MIC) values of 33.75 mu g mL(-1), 270 mu g mL-1 and 135 mu g mL(-1), respectively. These results together indicated that there were multiplicate ALF isoforms coexist in E. sinensis, and the diversity of the immune effector molecules in innate immune system, such as ALF, helps Chinese mitten crab to deal with various pathogens in the aquatic environment.
KeywordEriocheir Sinensis Anti-lipopolysaccharide Factor Listonella Anguillarum Bacillus Subtilis Antimicrobial Activity
DOI10.5897/AJB11.2017
Indexed BySCI
Language英语
WOS IDWOS:000298556200004
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/32942
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Leilei,Zhang, Ying,Wang, Lingling,et al. A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(77):17678-17689.
APA Wang, Leilei.,Zhang, Ying.,Wang, Lingling.,Yang, Jialong.,Zhou, Zhi.,...&Song, Linsheng.(2011).A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(77),17678-17689.
MLA Wang, Leilei,et al."A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.77(2011):17678-17689.
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