Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease | |
| Mo Zhaolan1; Guo Dongsheng1,2; Mao Yunxiang3; Ye Xuhong3; Zou Yuxia1; Xiao Peng1; Hao Bin3 | |
| 2010 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 卷号 | 28期号:1页码:55-61 |
| 文章类型 | Article |
| 摘要 | We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain. This prtV gene encodes a putative protein of 918 amino acids, and is highly homologous to the V. cholerae prtV gene. We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar, lower protease activity against azocasein, and lower activity for four glycosidases. This prtV mutant strain also had increased activity for two esterases in its extracellular products, as analyzed by the API ZYM system. In addition, the prtV mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes. Infection experiments showed that the LD(50) of the prtV mutant strain increased by at least 1 log compared to the wild-type in turbot fish. We propose that prtV plays an important role in the pathogenesis of V. anguillarum. |
| 关键词 | Vibrio Anguillarum Prtv Proteolysis Hemolysis Mucus Ld(50) |
| DOI | 10.1007/s00343-010-9246-4 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275654100007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32868 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 3.Ocean Univ China, Qingdao 266003, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Mo Zhaolan,Guo Dongsheng,Mao Yunxiang,et al. Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(1):55-61. |
| APA | Mo Zhaolan.,Guo Dongsheng.,Mao Yunxiang.,Ye Xuhong.,Zou Yuxia.,...&Hao Bin.(2010).Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(1),55-61. |
| MLA | Mo Zhaolan,et al."Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.1(2010):55-61. |
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