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Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease
Mo Zhaolan1; Guo Dongsheng1,2; Mao Yunxiang3; Ye Xuhong3; Zou Yuxia1; Xiao Peng1; Hao Bin3
2010
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
Volume28Issue:1Pages:55-61
SubtypeArticle
AbstractWe cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain. This prtV gene encodes a putative protein of 918 amino acids, and is highly homologous to the V. cholerae prtV gene. We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar, lower protease activity against azocasein, and lower activity for four glycosidases. This prtV mutant strain also had increased activity for two esterases in its extracellular products, as analyzed by the API ZYM system. In addition, the prtV mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes. Infection experiments showed that the LD(50) of the prtV mutant strain increased by at least 1 log compared to the wild-type in turbot fish. We propose that prtV plays an important role in the pathogenesis of V. anguillarum.
KeywordVibrio Anguillarum Prtv Proteolysis Hemolysis Mucus Ld(50)
DOI10.1007/s00343-010-9246-4
Indexed BySCI
Language英语
WOS IDWOS:000275654100007
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Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/32868
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
3.Ocean Univ China, Qingdao 266003, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Mo Zhaolan,Guo Dongsheng,Mao Yunxiang,et al. Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(1):55-61.
APA Mo Zhaolan.,Guo Dongsheng.,Mao Yunxiang.,Ye Xuhong.,Zou Yuxia.,...&Hao Bin.(2010).Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(1),55-61.
MLA Mo Zhaolan,et al."Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.1(2010):55-61.
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