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Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease
Mo Zhaolan1; Guo Dongsheng1,2; Mao Yunxiang3; Ye Xuhong3; Zou Yuxia1; Xiao Peng1; Hao Bin3
2010
发表期刊CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号28期号:1页码:55-61
文章类型Article
摘要We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain. This prtV gene encodes a putative protein of 918 amino acids, and is highly homologous to the V. cholerae prtV gene. We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar, lower protease activity against azocasein, and lower activity for four glycosidases. This prtV mutant strain also had increased activity for two esterases in its extracellular products, as analyzed by the API ZYM system. In addition, the prtV mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes. Infection experiments showed that the LD(50) of the prtV mutant strain increased by at least 1 log compared to the wild-type in turbot fish. We propose that prtV plays an important role in the pathogenesis of V. anguillarum.
关键词Vibrio Anguillarum Prtv Proteolysis Hemolysis Mucus Ld(50)
DOI10.1007/s00343-010-9246-4
收录类别SCI
语种英语
WOS记录号WOS:000275654100007
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/32868
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
3.Ocean Univ China, Qingdao 266003, Peoples R China
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Mo Zhaolan,Guo Dongsheng,Mao Yunxiang,et al. Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(1):55-61.
APA Mo Zhaolan.,Guo Dongsheng.,Mao Yunxiang.,Ye Xuhong.,Zou Yuxia.,...&Hao Bin.(2010).Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(1),55-61.
MLA Mo Zhaolan,et al."Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.1(2010):55-61.
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