Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis

doi:10.1007/s10811-009-9427-8

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	Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis
	Su, Hai-Nan 1; Xie, Bin-Bin 1; Chen, Xiu-Lan 1; Wang, Jin-Xia 2; Zhang, Xi-Ying 1; Zhou, Bai-Cheng 1,2; Zhang, Yu-Zhong 1
	2010-02-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
卷号	22 期号:1 页码:65-70
文章类型	Article
摘要	Allophycocyanin (APC) is a minor component of phycobiliproteins in cyanobacteria and red algae. This paper describes a simple and inexpensive extracting method for isolating APC from Spirulina (Arthrospira) platensis with high efficiency. The crude phycobiliprotein extract was pretreated by ammonium sulfate fractionation. Then, by adding hydroxylapatite into crude phycobiliprotein extract dissolved in 20 mM phosphate buffer (pH 7.0), APC was selectively adsorbed by hydroxylapatite but C-phycocyanin (C-PC) was not. The hydroxylapatite was collected and APC was extracted from the crude phycobiliprotein extract. Then, the enriched APC was washed off from the hydroxylapatite using 100 mM phosphate buffer (pH 7.0). In this simple extracting method it was easy to remove C-PC and isolate APC in large amounts. The absorbance ratio A (650)/A (280) of extracted APC reached 2.0. The recovery yield was 70%, representing 4.61 mg center dot g(-1) wet weight. The extracted APC could be further purified by a simple anion-exchange chromatography with a pH gradient from 5.6 to 4.0. The absorbance ratio A (650)/A (280) of the purified APC reached 5.0, and the overall recovery yield was 43%, representing 2.83 mg center dot g(-1) wet weight. Its purity was confirmed by native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate-PAGE.
关键词	Allophycocyanin Purification Hydroxylapatite Anion-exchange Chromatography Spirulina Platensis
DOI	10.1007/s10811-009-9427-8
收录类别	SCI
语种	英语
WOS记录号	WOS:000274435300007
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32838
专题	实验海洋生物学重点实验室
作者单位	1.Shandong Univ, State Key Lab Microbial Technol, Marine Biotechnol Res Ctr, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Su, Hai-Nan,Xie, Bin-Bin,Chen, Xiu-Lan,et al. Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis[J]. JOURNAL OF APPLIED PHYCOLOGY,2010,22(1):65-70.
APA	Su, Hai-Nan.,Xie, Bin-Bin.,Chen, Xiu-Lan.,Wang, Jin-Xia.,Zhang, Xi-Ying.,...&Zhang, Yu-Zhong.(2010).Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis.JOURNAL OF APPLIED PHYCOLOGY,22(1),65-70.
MLA	Su, Hai-Nan,et al."Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis".JOURNAL OF APPLIED PHYCOLOGY 22.1(2010):65-70.

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