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Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis
Su, Hai-Nan1; Xie, Bin-Bin1; Chen, Xiu-Lan1; Wang, Jin-Xia2; Zhang, Xi-Ying1; Zhou, Bai-Cheng1,2; Zhang, Yu-Zhong1
2010-02-01
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
Volume22Issue:1Pages:65-70
SubtypeArticle
AbstractAllophycocyanin (APC) is a minor component of phycobiliproteins in cyanobacteria and red algae. This paper describes a simple and inexpensive extracting method for isolating APC from Spirulina (Arthrospira) platensis with high efficiency. The crude phycobiliprotein extract was pretreated by ammonium sulfate fractionation. Then, by adding hydroxylapatite into crude phycobiliprotein extract dissolved in 20 mM phosphate buffer (pH 7.0), APC was selectively adsorbed by hydroxylapatite but C-phycocyanin (C-PC) was not. The hydroxylapatite was collected and APC was extracted from the crude phycobiliprotein extract. Then, the enriched APC was washed off from the hydroxylapatite using 100 mM phosphate buffer (pH 7.0). In this simple extracting method it was easy to remove C-PC and isolate APC in large amounts. The absorbance ratio A (650)/A (280) of extracted APC reached 2.0. The recovery yield was 70%, representing 4.61 mg center dot g(-1) wet weight. The extracted APC could be further purified by a simple anion-exchange chromatography with a pH gradient from 5.6 to 4.0. The absorbance ratio A (650)/A (280) of the purified APC reached 5.0, and the overall recovery yield was 43%, representing 2.83 mg center dot g(-1) wet weight. Its purity was confirmed by native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate-PAGE.
KeywordAllophycocyanin Purification Hydroxylapatite Anion-exchange Chromatography Spirulina Platensis
DOI10.1007/s10811-009-9427-8
Indexed BySCI
Language英语
WOS IDWOS:000274435300007
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/32838
Collection实验海洋生物学重点实验室
Affiliation1.Shandong Univ, State Key Lab Microbial Technol, Marine Biotechnol Res Ctr, Jinan 250100, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Su, Hai-Nan,Xie, Bin-Bin,Chen, Xiu-Lan,et al. Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis[J]. JOURNAL OF APPLIED PHYCOLOGY,2010,22(1):65-70.
APA Su, Hai-Nan.,Xie, Bin-Bin.,Chen, Xiu-Lan.,Wang, Jin-Xia.,Zhang, Xi-Ying.,...&Zhang, Yu-Zhong.(2010).Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis.JOURNAL OF APPLIED PHYCOLOGY,22(1),65-70.
MLA Su, Hai-Nan,et al."Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis".JOURNAL OF APPLIED PHYCOLOGY 22.1(2010):65-70.
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