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Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis
Su, Hai-Nan1; Xie, Bin-Bin1; Chen, Xiu-Lan1; Wang, Jin-Xia2; Zhang, Xi-Ying1; Zhou, Bai-Cheng1,2; Zhang, Yu-Zhong1
2010-02-01
发表期刊JOURNAL OF APPLIED PHYCOLOGY
卷号22期号:1页码:65-70
文章类型Article
摘要Allophycocyanin (APC) is a minor component of phycobiliproteins in cyanobacteria and red algae. This paper describes a simple and inexpensive extracting method for isolating APC from Spirulina (Arthrospira) platensis with high efficiency. The crude phycobiliprotein extract was pretreated by ammonium sulfate fractionation. Then, by adding hydroxylapatite into crude phycobiliprotein extract dissolved in 20 mM phosphate buffer (pH 7.0), APC was selectively adsorbed by hydroxylapatite but C-phycocyanin (C-PC) was not. The hydroxylapatite was collected and APC was extracted from the crude phycobiliprotein extract. Then, the enriched APC was washed off from the hydroxylapatite using 100 mM phosphate buffer (pH 7.0). In this simple extracting method it was easy to remove C-PC and isolate APC in large amounts. The absorbance ratio A (650)/A (280) of extracted APC reached 2.0. The recovery yield was 70%, representing 4.61 mg center dot g(-1) wet weight. The extracted APC could be further purified by a simple anion-exchange chromatography with a pH gradient from 5.6 to 4.0. The absorbance ratio A (650)/A (280) of the purified APC reached 5.0, and the overall recovery yield was 43%, representing 2.83 mg center dot g(-1) wet weight. Its purity was confirmed by native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate-PAGE.
关键词Allophycocyanin Purification Hydroxylapatite Anion-exchange Chromatography Spirulina Platensis
DOI10.1007/s10811-009-9427-8
收录类别SCI
语种英语
WOS记录号WOS:000274435300007
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/32838
专题实验海洋生物学重点实验室
作者单位1.Shandong Univ, State Key Lab Microbial Technol, Marine Biotechnol Res Ctr, Jinan 250100, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
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Su, Hai-Nan,Xie, Bin-Bin,Chen, Xiu-Lan,et al. Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis[J]. JOURNAL OF APPLIED PHYCOLOGY,2010,22(1):65-70.
APA Su, Hai-Nan.,Xie, Bin-Bin.,Chen, Xiu-Lan.,Wang, Jin-Xia.,Zhang, Xi-Ying.,...&Zhang, Yu-Zhong.(2010).Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis.JOURNAL OF APPLIED PHYCOLOGY,22(1),65-70.
MLA Su, Hai-Nan,et al."Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis".JOURNAL OF APPLIED PHYCOLOGY 22.1(2010):65-70.
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