Improvement of polyploidy induction in Eriocheir sinensis

IOCAS-IR > 实验海洋生物学重点实验室

	Improvement of polyploidy induction in Eriocheir sinensis
	Cui, ZX; Xiang, JH; Zhou, LH; Cai, NN; Song, LS
	2004
发表期刊	ACTA OCEANOLOGICA SINICA
ISSN	0253-505X
卷号	23 期号:4 页码:725-732
文章类型	Article
摘要	An incubating temperature of 15 degreesC is used to induce triploidy in Etiocheir sinensis through inhibition of the release of polar body H, and that of 18 degreesC to induce tetraploidy through inhibition of the first cleavage. Flow cytometry is used to identify the ploidy in different developmental stages. For induction of triploidy in fertilized eggs in vitro, the highest induction rate observed in blastula by cytochalasin B, 6-DMAP and KCI is 49.1%, 51.7% and 77.5%, respectively. In the KCI treatment of pregnant crabs with the fertilized eggs, the highest triploid induction rate observed in the zoea is 85.3%. For induction of tetraploidy, the highest induction rate observed in the blastula by cytochaslasin 13, 6-DMAP and KCI is 50.3%, 54.9% and 79.8% respectively. In the KCI treatment of pregnant crabs with the fertilized eggs, the highest induction rate in zoea is 27.3%. Through this study such difficulty as in vitro culture is overcome. Triploid zoea Etiocheir sinensis has been developed for the first time. The induction rate of tetraploid zoea has also been greatly improved.; An incubating temperature of 15 degreesC is used to induce triploidy in Etiocheir sinensis through inhibition of the release of polar body H, and that of 18 degreesC to induce tetraploidy through inhibition of the first cleavage. Flow cytometry is used to identify the ploidy in different developmental stages. For induction of triploidy in fertilized eggs in vitro, the highest induction rate observed in blastula by cytochalasin B, 6-DMAP and KCI is 49.1%, 51.7% and 77.5%, respectively. In the KCI treatment of pregnant crabs with the fertilized eggs, the highest triploid induction rate observed in the zoea is 85.3%. For induction of tetraploidy, the highest induction rate observed in the blastula by cytochaslasin 13, 6-DMAP and KCI is 50.3%, 54.9% and 79.8% respectively. In the KCI treatment of pregnant crabs with the fertilized eggs, the highest induction rate in zoea is 27.3%. Through this study such difficulty as in vitro culture is overcome. Triploid zoea Etiocheir sinensis has been developed for the first time. The induction rate of tetraploid zoea has also been greatly improved.
关键词	Eriocheir Sinensis Triploid Tetraploid Zoea
学科领域	Oceanography
收录类别	SCI
语种	英语
WOS记录号	WOS:000226085700016
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3186
专题	实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, EMBL, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Cui, ZX,Xiang, JH,Zhou, LH,et al. Improvement of polyploidy induction in Eriocheir sinensis[J]. ACTA OCEANOLOGICA SINICA,2004,23(4):725-732.
APA	Cui, ZX,Xiang, JH,Zhou, LH,Cai, NN,&Song, LS.(2004).Improvement of polyploidy induction in Eriocheir sinensis.ACTA OCEANOLOGICA SINICA,23(4),725-732.
MLA	Cui, ZX,et al."Improvement of polyploidy induction in Eriocheir sinensis".ACTA OCEANOLOGICA SINICA 23.4(2004):725-732.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
cui-Improvement of p（91KB）			限制开放	--	浏览