The preparation and bioactivity research of agaro-oligosaccharides

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	The preparation and bioactivity research of agaro-oligosaccharides
	Chen, HM ; Zheng, L ; Yan, XJ
	2005
发表期刊	FOOD TECHNOLOGY AND BIOTECHNOLOGY
ISSN	1330-9862
卷号	43 期号:1 页码:29-36
文章类型	Article
摘要	Agaro-oligosaccharides were hydrolytically obtained from agar using hydrochloric acid, citric acid, and cationic exchange resin (solid acid). The FT-IR and NMR data showed that the hydrolysate has the structure of agaro-oligomers. Orthogonal matrix method was applied to optimize the preparation conditions based on alpha-naphthylamine end-labeled HPLC analysis method. The optimal way for oligosaccharides with different degree of polymerization (DP) was achieved by using solid acid degradation, which could give high yield and avoid solution neutralization process. Agaro-oligosaccharides with high purity were consequently obtained by activated carbon column isolation. Furthermore, the antioxidant and alpha-glucosidase inhibitory activity of three fractions were also investigated. The result indicated that 8% ethanol-eluted fraction showed highest activity against alpha-glucosidase with IC50 of 8.84 mg/mL, while 25% ethanol-eluted fraction possessed excellent antioxidant ability.; Agaro-oligosaccharides were hydrolytically obtained from agar using hydrochloric acid, citric acid, and cationic exchange resin (solid acid). The FT-IR and NMR data showed that the hydrolysate has the structure of agaro-oligomers. Orthogonal matrix method was applied to optimize the preparation conditions based on alpha-naphthylamine end-labeled HPLC analysis method. The optimal way for oligosaccharides with different degree of polymerization (DP) was achieved by using solid acid degradation, which could give high yield and avoid solution neutralization process. Agaro-oligosaccharides with high purity were consequently obtained by activated carbon column isolation. Furthermore, the antioxidant and alpha-glucosidase inhibitory activity of three fractions were also investigated. The result indicated that 8% ethanol-eluted fraction showed highest activity against alpha-glucosidase with IC50 of 8.84 mg/mL, while 25% ethanol-eluted fraction possessed excellent antioxidant ability.
关键词	Agaro-oligosaccharides Orthogonal Matrix Hplc Acid Degradation Antioxidant Alpha-glucosidase
学科领域	Biotechnology & Applied Microbiology ; Food Science & Technology
收录类别	SCI
语种	英语
WOS记录号	WOS:000227947900004
引用统计	被引频次：52[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3084
专题	实验海洋生物学重点实验室
作者单位	1.Ningbo Univ, Key Lab Marine Biotechnol, Ningbo 315211, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Chen, HM,Zheng, L,Yan, XJ. The preparation and bioactivity research of agaro-oligosaccharides[J]. FOOD TECHNOLOGY AND BIOTECHNOLOGY,2005,43(1):29-36.
APA	Chen, HM,Zheng, L,&Yan, XJ.(2005).The preparation and bioactivity research of agaro-oligosaccharides.FOOD TECHNOLOGY AND BIOTECHNOLOGY,43(1),29-36.
MLA	Chen, HM,et al."The preparation and bioactivity research of agaro-oligosaccharides".FOOD TECHNOLOGY AND BIOTECHNOLOGY 43.1(2005):29-36.

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