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Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses
You Feng1; Wang Wei1; Xu Dongdong1; Zhu Xiangping1,2; Ni Jing1; Wu Zhihao1; Xu Yongli1; Wang Xincheng1; Zhang Peijun1
2009-06-01
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
Volume27Issue:2Pages:317-323
SubtypeArticle
AbstractThe hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.; The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.
KeywordArtificial Hybridization Paralichthys Olivaceus (Temminck Et Schlegel) Kareius Bicoloratus (Basilewsky) Karyotype Allozyme Rapd
Subject AreaLimnology ; Oceanography
DOI10.1007/s00343-009-9134-y
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000267970300016
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/3062
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Qingdao Agr Univ, Qingdao 266109, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
You Feng,Wang Wei,Xu Dongdong,et al. Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(2):317-323.
APA You Feng.,Wang Wei.,Xu Dongdong.,Zhu Xiangping.,Ni Jing.,...&Zhang Peijun.(2009).Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(2),317-323.
MLA You Feng,et al."Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.2(2009):317-323.
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