Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses

doi:10.1007/s00343-009-9134-y

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	Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses
	You Feng 1; Wang Wei 1; Xu Dongdong 1; Zhu Xiangping 1,2; Ni Jing 1; Wu Zhihao 1; Xu Yongli 1; Wang Xincheng 1; Zhang Peijun 1
	2009-06-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	27 期号:2 页码:317-323
文章类型	Article
摘要	The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.; The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.
关键词	Artificial Hybridization Paralichthys Olivaceus (Temminck Et Schlegel) Kareius Bicoloratus (Basilewsky) Karyotype Allozyme Rapd
学科领域	Limnology ; Oceanography
DOI	10.1007/s00343-009-9134-y
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000267970300016
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3062
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Qingdao Agr Univ, Qingdao 266109, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	You Feng,Wang Wei,Xu Dongdong,et al. Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(2):317-323.
APA	You Feng.,Wang Wei.,Xu Dongdong.,Zhu Xiangping.,Ni Jing.,...&Zhang Peijun.(2009).Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(2),317-323.
MLA	You Feng,et al."Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.2(2009):317-323.

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