| Structural characterization and expression analysis of a novel cysteine protease inhibitor from Haliotis discus hannai Ino | |
| Ding, Jianfeng2; Li, Li1; Zhang, Guofan1 | |
| 2015-02-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
 |
| 卷号 | 42期号:2页码:379-383 |
| 文章类型 | Article |
| 摘要 | The sequence of the cysteine protease inhibitor gene of Haliotis discus hannai (designated HdCpi) was determined using the RACE method. The full-length HdCpi cDNA is 1049 bp long, and contains an open reading frame of 813 bp, encoding a 271-amino-acid protein with a calculated molecular mass of 29.83 kDa and an isoelectric point of 8.57. The deduced amino acid sequence of HdCpi contains two cystatin-like domains, and each has the structural features of the cystatin family, including three evolutionarily conserved motifs known to interact with the active sites of cysteine peptidases: the Gly residue at the N-terminus (Gly(65) and Gly(160)), the Gln-X-Val-X-Gly motif (Q(106)IVSO(110) and Q(202)VVAG(206),) and the less conserved motif at the C-terminus ((SW137)-W-136 and A(254)W(255)). Many putative transcription-factor-binding sites involved in the immune system and cancer occur in the promoter region of HdCpi. Quantitative real-time RT-PCR detected HdCpi expression in all the tissues examined and in the gills of abalone challenged with the bacterium Vibrio anguillarum. HdCpi transcripts were expressed in the mantle, gill, digestive tract, hemocytes, and muscle, and increased HdCpi expression was observed after bacterial stimulation. These results suggest that HdCpi is a biologically active protease inhibitor that is likely to be involved in the antibacterial response of the abalone. (C) 2014 Elsevier Ltd. All rights reserved. |
| 关键词 | Haliotis Discus Hannai Cysteine Protease Inhibitor Cpi Immunity |
| DOI | 10.1016/j.fsi.2014.11.005 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000348621700020 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/25366 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Jianfeng,Li, Li,Zhang, Guofan. Structural characterization and expression analysis of a novel cysteine protease inhibitor from Haliotis discus hannai Ino[J]. FISH & SHELLFISH IMMUNOLOGY,2015,42(2):379-383. |
| APA | Ding, Jianfeng,Li, Li,&Zhang, Guofan.(2015).Structural characterization and expression analysis of a novel cysteine protease inhibitor from Haliotis discus hannai Ino.FISH & SHELLFISH IMMUNOLOGY,42(2),379-383. |
| MLA | Ding, Jianfeng,et al."Structural characterization and expression analysis of a novel cysteine protease inhibitor from Haliotis discus hannai Ino".FISH & SHELLFISH IMMUNOLOGY 42.2(2015):379-383. |
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