Effect of Fe3+ on the growth and lipid content of Isochrysis galbana

doi:10.1007/s00343-014-3110-x

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	Effect of Fe3+ on the growth and lipid content of Isochrysis galbana
	Liu Zhiyuan 1; Wang Guangce 2; Wang, GC (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
	2014
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号	32 期号:1 页码:47-53
文章类型	Article
摘要	Inducing lipid accumulation in microalgae cells without suppressing cell growth is vital to the economical production of biodiesel from microalgae. In two experiments, we demonstrate that the cell concentration and lipid content of marine microalgae Isochrysis galbana depend upon the iron concentration in the growth media. In Experiment I, adding chelated FeCl3 to the medium at the late exponential growth phase prolonged this phase and increased the lipid content in I. galbana cells. The final cell density and lipid content of I. galbana supplemented with chelated FeCl3 was approximately 2 and 1.65 times higher than that of non-supplemented cultures, respectively. In Experiment II, I. galbana cells in the late exponential phase were collected and re-inoculated into new media containing Fe3+ at various concentrations. The final cell concentration and lipid content were maximized at the highest iron concentration (38% biomass by dry weight at 1.2x10(-5) mol/L Fe3+). In this study, intracellular neutral lipid storage was evaluated by fluorescent spectrophotometry using fluorochrome Nile red, and the measurement conditions were optimized.
关键词	Biodiesel Cell Density Iron Microalgae Nile Red
学科领域	Marine & Freshwater Biology ; Oceanography
DOI	10.1007/s00343-014-3110-x
收录类别	SCI
语种	英语
WOS记录号	WOS:000332003500005
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24108
专题	实验海洋生物学重点实验室
通讯作者	Wang, GC (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
作者单位	1.Hainan Univ, Coll Oceanol, Haikou 570228, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Liu Zhiyuan,Wang Guangce,Wang, GC . Effect of Fe3+ on the growth and lipid content of Isochrysis galbana[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(1):47-53.
APA	Liu Zhiyuan,Wang Guangce,&Wang, GC .(2014).Effect of Fe3+ on the growth and lipid content of Isochrysis galbana.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(1),47-53.
MLA	Liu Zhiyuan,et al."Effect of Fe3+ on the growth and lipid content of Isochrysis galbana".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.1(2014):47-53.

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