Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Cryptochrome PtCPF1 regulates high temperature acclimation of marine diatoms through coordination of iron and phosphorus uptake | |
Gao, Shan1,2,3,4; Yang, Wenting1,2,3,4; Li, Xin1,2,3,4,5 ; Zhou, Lu1,2,3,4; Liu, Xuehua1,2,3,4; Wu, Songcui1,2,3,4; Wang, Lijun1,2,3,4; Wang, Guangce1,2,3,4
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| 2024-01-08 | |
| Source Publication | ISME JOURNAL
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| ISSN | 1751-7362 |
| Volume | 18Issue:1Pages:14 |
| Corresponding Author | Wang, Guangce(gcwang@qdio.ac.cn) |
| Abstract | Increasing ocean temperatures threaten the productivity and species composition of marine diatoms. High temperature response and regulation are important for the acclimation of marine diatoms to such environments. However, the molecular mechanisms behind their acclimation to high temperature are still largely unknown. In this study, the abundance of PtCPF1 homologs (a member of the cryptochrome-photolyase family in the model diatom Phaeodactylum tricornutum) transcripts in marine phytoplankton is shown to increase with rising temperature based on Tara Oceans datasets. Moreover, the expression of PtCPF1 in P. tricornutum at high temperature (26 degrees C) was much higher than that at optimum temperature (20 degrees C). Deletion of PtCPF1 in P. tricornutum disrupted the expression of genes encoding two phytotransferrins (ISIP2A and ISIP1) and two Na+/P co-transporters (PHATRDRAFT_47667 and PHATRDRAFT_40433) at 26 degrees C. This further impacted the uptake of Fe and P, and eventually caused the arrest of cell division. Gene expression, Fe and P uptake, and cell division were restored by rescue with the native PtCPF1 gene. Furthermore, PtCPF1 interacts with two putative transcription factors (BolA and TF IIA) that potentially regulate the expression of genes encoding phytotransferrins and Na+/P co-transporters. To the best of our knowledge, this is the first study to reveal PtCPF1 as an essential regulator in the acclimation of marine diatoms to high temperature through the coordination of Fe and P uptake. Therefore, these findings help elucidate how marine diatoms acclimate to high temperature. |
| Keyword | cryptochrome diatoms high temperature iron and phosphorus uptake |
| DOI | 10.1093/ismejo/wrad019 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[4227614642376093] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; Project of Science and Technology Bureau of Nantong[MS2023012] ; China Agriculture Research System of MOF and MARA[CARS-50] ; Key Deployment Project of the Centre for Ocean Mega-Research of Science, the Chinese Academy of Sciences[COMS2019Q02] ; National Key R&D Program of China[2018YFD0901500] |
| WOS Research Area | Environmental Sciences & Ecology ; Microbiology |
| WOS Subject | Ecology ; Microbiology |
| WOS ID | WOS:001155584700001 |
| Publisher | OXFORD UNIV PRESS |
| WOS Keyword | PHAEODACTYLUM-TRICORNUTUM ; LIGHT ; RESPONSES ; PROTEIN ; FAMILY ; PHYTOPLANKTON ; STRESS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/184408 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Wang, Guangce |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Gao, Shan,Yang, Wenting,Li, Xin,et al. Cryptochrome PtCPF1 regulates high temperature acclimation of marine diatoms through coordination of iron and phosphorus uptake[J]. ISME JOURNAL,2024,18(1):14. |
| APA | Gao, Shan.,Yang, Wenting.,Li, Xin.,Zhou, Lu.,Liu, Xuehua.,...&Wang, Guangce.(2024).Cryptochrome PtCPF1 regulates high temperature acclimation of marine diatoms through coordination of iron and phosphorus uptake.ISME JOURNAL,18(1),14. |
| MLA | Gao, Shan,et al."Cryptochrome PtCPF1 regulates high temperature acclimation of marine diatoms through coordination of iron and phosphorus uptake".ISME JOURNAL 18.1(2024):14. |
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