Cryptochrome PtCPF1 regulates high temperature acclimation of marine diatoms through coordination of iron and phosphorus uptake

doi:10.1093/ismejo/wrad019

IOCAS-IR > 实验海洋生物学重点实验室

	Cryptochrome PtCPF1 regulates high temperature acclimation of marine diatoms through coordination of iron and phosphorus uptake
	Gao, Shan 1,2,3,4; Yang, Wenting 1,2,3,4; Li, Xin1,2,3,4,5 ; Zhou, Lu 1,2,3,4; Liu, Xuehua 1,2,3,4; Wu, Songcui1,2,3,4 ; Wang, Lijun 1,2,3,4; Wang, Guangce 1,2,3,4
	2024-01-08
发表期刊	ISME JOURNAL
ISSN	1751-7362
卷号	18 期号:1 页码:14
通讯作者	Wang, Guangce(gcwang@qdio.ac.cn)
摘要	Increasing ocean temperatures threaten the productivity and species composition of marine diatoms. High temperature response and regulation are important for the acclimation of marine diatoms to such environments. However, the molecular mechanisms behind their acclimation to high temperature are still largely unknown. In this study, the abundance of PtCPF1 homologs (a member of the cryptochrome-photolyase family in the model diatom Phaeodactylum tricornutum) transcripts in marine phytoplankton is shown to increase with rising temperature based on Tara Oceans datasets. Moreover, the expression of PtCPF1 in P. tricornutum at high temperature (26 degrees C) was much higher than that at optimum temperature (20 degrees C). Deletion of PtCPF1 in P. tricornutum disrupted the expression of genes encoding two phytotransferrins (ISIP2A and ISIP1) and two Na+/P co-transporters (PHATRDRAFT_47667 and PHATRDRAFT_40433) at 26 degrees C. This further impacted the uptake of Fe and P, and eventually caused the arrest of cell division. Gene expression, Fe and P uptake, and cell division were restored by rescue with the native PtCPF1 gene. Furthermore, PtCPF1 interacts with two putative transcription factors (BolA and TF IIA) that potentially regulate the expression of genes encoding phytotransferrins and Na+/P co-transporters. To the best of our knowledge, this is the first study to reveal PtCPF1 as an essential regulator in the acclimation of marine diatoms to high temperature through the coordination of Fe and P uptake. Therefore, these findings help elucidate how marine diatoms acclimate to high temperature.
关键词	cryptochrome diatoms high temperature iron and phosphorus uptake
DOI	10.1093/ismejo/wrad019
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[4227614642376093] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; Project of Science and Technology Bureau of Nantong[MS2023012] ; China Agriculture Research System of MOF and MARA[CARS-50] ; Key Deployment Project of the Centre for Ocean Mega-Research of Science, the Chinese Academy of Sciences[COMS2019Q02] ; National Key R&D Program of China[2018YFD0901500]
WOS研究方向	Environmental Sciences & Ecology ; Microbiology
WOS类目	Ecology ; Microbiology
WOS记录号	WOS:001155584700001
出版者	OXFORD UNIV PRESS
WOS关键词	PHAEODACTYLUM-TRICORNUTUM ; LIGHT ; RESPONSES ; PROTEIN ; FAMILY ; PHYTOPLANKTON ; STRESS
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184408
专题	实验海洋生物学重点实验室
通讯作者	Wang, Guangce
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Gao, Shan,Yang, Wenting,Li, Xin,et al. Cryptochrome PtCPF1 regulates high temperature acclimation of marine diatoms through coordination of iron and phosphorus uptake[J]. ISME JOURNAL,2024,18(1):14.
APA	Gao, Shan.,Yang, Wenting.,Li, Xin.,Zhou, Lu.,Liu, Xuehua.,...&Wang, Guangce.(2024).Cryptochrome PtCPF1 regulates high temperature acclimation of marine diatoms through coordination of iron and phosphorus uptake.ISME JOURNAL,18(1),14.
MLA	Gao, Shan,et al."Cryptochrome PtCPF1 regulates high temperature acclimation of marine diatoms through coordination of iron and phosphorus uptake".ISME JOURNAL 18.1(2024):14.