Genome-Wide Association Analysis of Heat Tolerance in F<sub>2</sub> Progeny from the Hybridization between Two Congeneric Oyster Species

doi:10.3390/ijms25010125

IOCAS-IR

	Genome-Wide Association Analysis of Heat Tolerance in F₂ Progeny from the Hybridization between Two Congeneric Oyster Species
	Du, Mingyang 1,2,3; Jiang, Zhuxiang 1,2,3; Wang, Chaogang 1,2,3; Wei, Chenchen 1,2; Li, Qingyuan 1,2,3; Cong, Rihao 1,2,4; Wang, Wei 1,4,5; Zhang, Guofan 1,2,4,6; Li, Li 1,3,4,5,6; Dobretsov, Sergey
	2024
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1661-6596
卷号	25 期号:1 页码:17
通讯作者	Li, Li(lili@qdio.ac.cn)
摘要	As the world's largest farmed marine animal, oysters have enormous economic and ecological value. However, mass summer mortality caused by high temperature poses a significant threat to the oyster industry. To investigate the molecular mechanisms underlying heat adaptation and improve the heat tolerance ability in the oyster, we conducted genome-wide association analysis (GWAS) analysis on the F-2 generation derived from the hybridization of relatively heat-tolerant Crassostrea angulata female and heat-sensitive Crassostrea gigas male, which are the dominant cultured species in southern and northern China, respectively. Acute heat stress experiment (semi-lethal temperature 42 degrees C) demonstrated that the F-2 population showed differentiation in heat tolerance, leading to extremely differentiated individuals (approximately 20% of individuals die within the first four days with 10% survival after 14 days). Genome resequencing and GWAS of the two divergent groups had identified 18 significant SNPs associated with heat tolerance, with 26 candidate genes located near these SNPs. Eleven candidate genes that may associate with the thermal resistance were identified, which were classified into five categories: temperature sensor (Trpm2), transcriptional factor (Gata3), protein ubiquitination (Ube2h, Usp50, Uchl3), heat shock subfamily (Dnajc17, Dnaja1), and transporters (Slc16a9, Slc16a14, Slc16a9, Slc16a2). The expressional differentiation of the above genes between C. gigas and C. angulata under sublethal temperature (37 degrees C) further supports their crucial role in coping with high temperature. Our results will contribute to understanding the molecular mechanisms underlying heat tolerance, and provide genetic markers for heat-resistance breeding in the oyster industry.
关键词	oysters heat tolerance GWAS Crassostrea gigas Crassostrea angulata F-2 progeny
DOI	10.3390/ijms25010125
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:001141408700001
出版者	MDPI
WOS关键词	CRASSOSTREA-GIGAS ; SUMMER MORTALITY ; JAPANESE FLOUNDER ; STRESS ; TEMPERATURE ; RESISTANCE ; NORTH ; CLASSIFICATION ; ADAPTATION ; SELECTION
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183625
通讯作者	Li, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 2.Laoshan Lab, Lab Marine Biol & Biotechnol, Qingdao 266100, Peoples R China 3.Univ Chinese Acad Sci, Beijing 101408, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 5.Laoshan Lab, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266100, Peoples R China 6.Inst Hydrobiol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Du, Mingyang,Jiang, Zhuxiang,Wang, Chaogang,et al. Genome-Wide Association Analysis of Heat Tolerance in F₂ Progeny from the Hybridization between Two Congeneric Oyster Species[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2024,25(1):17.
APA	Du, Mingyang.,Jiang, Zhuxiang.,Wang, Chaogang.,Wei, Chenchen.,Li, Qingyuan.,...&Dobretsov, Sergey.(2024).Genome-Wide Association Analysis of Heat Tolerance in F₂ Progeny from the Hybridization between Two Congeneric Oyster Species.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,25(1),17.
MLA	Du, Mingyang,et al."Genome-Wide Association Analysis of Heat Tolerance in F₂ Progeny from the Hybridization between Two Congeneric Oyster Species".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 25.1(2024):17.