Female-specific genomic regions and molecular sex identification of the clearhead icefish (Protosalanx hyalocranius)

doi:10.1186/s12864-021-07830-9

IOCAS-IR

	Female-specific genomic regions and molecular sex identification of the clearhead icefish (Protosalanx hyalocranius)
	Xing, Teng-Fei 1,2,3,4; Li, Yu-Long 1,2,3; Liu, Jin-Xian 1,2,3
	2021-07-02
发表期刊	BMC GENOMICS
ISSN	1471-2164
卷号	22 期号:1 页码:9
通讯作者	Li, Yu-Long(lyl@qdio.ac.cn)
摘要	Background: The clearhead icefish, Protosalanx hyalocranius, is an economically important fishery species in China. Since 1980s, P. hyalocranius was widely introduced into lakes and reservoirs of northern China for aquaculture. However, the lack of a rapid and cost-effective sex identification method based on sex specific genetic markers has hindered study on sex determination mechanisms and breeding applications. Results: Female-specific genomic regions were discovered by comparing whole genome re-sequencing data of both males and females. Two female-specific genomic regions larger than 50 bp were identified, and one (598 bp) contained a putative FOXI gene, which was paralogous to another FOXI gene with sex-associated SNPs. The two FOXI sequences displayed significant length difference with nine deletions of total length of 230 bp. This deletion-type structural variation could be easily and efficiently detected by traditional PCR and agarose gel electrophoresis with one 569 bp band for males and two bands (569 and 339 bp) for females, which were validated in 50 females and 40 males with known phenotypic sexes. Conclusions: The results provided structural genomic evidence for the ZZ/ZW sex determination system in P. hyalocranius discovered in our previous study with association analysis of SNPs. Moreover, the female-specific markers and rapid and cost-effective PCR-based genetic sex identification method should have applications in further studies of sex determination mechanism for this species.
关键词	Clearhead icefish Female-specific marker ZZ/ZW sex determination system Genetic sex identification
DOI	10.1186/s12864-021-07830-9
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41906080] ; National Natural Science Foundation of China[41676137]
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号	WOS:000671729100002
出版者	BMC
WOS关键词	AFLP MARKERS ; DNA MARKERS ; SEQUENCE ; FISH ; DIFFERENTIATION ; SALANGIDAE ; DIMORPHISM ; DIVERSITY ; GROWTH ; CHINA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176971
通讯作者	Li, Yu-Long
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Xing, Teng-Fei,Li, Yu-Long,Liu, Jin-Xian. Female-specific genomic regions and molecular sex identification of the clearhead icefish (Protosalanx hyalocranius)[J]. BMC GENOMICS,2021,22(1):9.
APA	Xing, Teng-Fei,Li, Yu-Long,&Liu, Jin-Xian.(2021).Female-specific genomic regions and molecular sex identification of the clearhead icefish (Protosalanx hyalocranius).BMC GENOMICS,22(1),9.
MLA	Xing, Teng-Fei,et al."Female-specific genomic regions and molecular sex identification of the clearhead icefish (Protosalanx hyalocranius)".BMC GENOMICS 22.1(2021):9.