IOCAS-IR  > 海洋生态与环境科学重点实验室
Adaption to hydrogen sulfide-rich environments: Strategies for active detoxification in deep-sea symbiotic mussels, Gigantidas platifrons
Sun, Yan1,2,3,4; Wang, Minxiao1,2,3,4; Zhong, Zhaoshan1,2,3,4; Chen, Hao1,2,3,4; Wang, Hao1,2,3,4; Zhou, Li1,2,3,4; Cao, Lei1,2,3,4; Fu, Lulu1,2,3,4; Zhang, Huan1,2,3,4; Lian, Chao1,2,3,4; Sun, Song1,2,3,4,5; Li, Chaolun1,2,3,4,5
2022-01-15
Source PublicationSCIENCE OF THE TOTAL ENVIRONMENT
ISSN0048-9697
Volume804Pages:13
Corresponding AuthorSun, Song(sunsong@qdio.ac.cn) ; Li, Chaolun(lcl@qdio.ac.cn)
AbstractThe deep-sea mussel Gigantidas plantifrons is a representative species that relies on nutrition provided by chemoautotrophic endosymbiotic bacteria to survive in both hydrothermal vent and methane seep environments. However, vent and seep habitats have distinct geochemical features, with vents being more harsh than seeps because of abundant toxic chemical substances, particularly hydrogen sulfide (H2S). Until now, the adaptive strategies of G. platifrons in a heterogeneous environment and their sulfide detoxification mechanisms are still unclear. Herein, we conducted 16S rDNA sequencing and metatranscriptome sequencing of G. plan:trans collected from a methane seep at Formosa Ridge in the South China Sea and a hydrothermal vent at lheya North Knoll in the Mid-Okinawa Trough to provide a model for understanding environmental adaption and sulfide detoxification mechanisms, and a three-day laboratory controlled Na2S stress experiment to test the transcriptomic responses under sulfide stress. The results revealed the active detoxification of sulfide in G. platifrons gills. First, epibiotic Campylobacterota bacteria were more abundant in vent mussels and contributed to environmental adaptation by active oxidation of extracellular H2S. Notably, a key sulfide-oxidizing gene, sulfide:quinone oxidoreductase (sqr), derived from the methanotrophic endosymbiont, was significantly upregulated in vent mussels, indicating the oxidization of intracellular sulfide by the endosymbiont. In addition, transcriptomic comparison further suggested that genes involved in oxidative phosphorylation and mitochondrial sulfide oxidization pathway played important roles in the sulfide tolerance of the host mussels. Moreover, uanscriptomic analysis of Na2S stressed mussels confirmed the upregulation of oxidative phosphorylation and sulfide oxidization genes in response to sulfide exposure. Overall, this study provided a systematic transcriptional analysis of both the active bacterial community members and the host mussels, suggesting that the epibionts, endosymbionts, and mussel host collaborated on sulfide detoxification from extracellular to intracellular space to adapt to harsh H2S-rich environments. (C) 2021 Published by Elsevier B.V.
KeywordSulfide detoxification Deep-sea adaptation Bathymodiolinae mussels Sulfide:quinone oxidoreductase Symbionts Mitochondria
DOI10.1016/j.scitotenv.2021.150054
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2018YFC0310800] ; National Key Research and Development Program of China[2017YFC1404401] ; National Natural Science Foundation of China[42030407] ; National Natural Science Foundation of China[42076091] ; National Natural Science Foundation of China[41806200] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050303] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; Open Research Project of National Major Science & Technology Infrastructure[NMSTI-KEXUE2017K01] ; Open Research Project of National Major Science & Technology Infrastructure[KEXUE2021GH01] ; Open Research Project of National Major Science & Technology Infrastructure[KEXUE2019GZ02] ; Open Research Project of National Major Science & Technology Infrastructure[KEXUE2019GZ06] ; Open Research Project of National Major Science & Technology Infrastructure[KEXUE2018G16]
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000701855500014
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/176492
Collection海洋生态与环境科学重点实验室
Corresponding AuthorSun, Song; Li, Chaolun
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
5.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
First Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS;  Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS;  Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Sun, Yan,Wang, Minxiao,Zhong, Zhaoshan,et al. Adaption to hydrogen sulfide-rich environments: Strategies for active detoxification in deep-sea symbiotic mussels, Gigantidas platifrons[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,804:13.
APA Sun, Yan.,Wang, Minxiao.,Zhong, Zhaoshan.,Chen, Hao.,Wang, Hao.,...&Li, Chaolun.(2022).Adaption to hydrogen sulfide-rich environments: Strategies for active detoxification in deep-sea symbiotic mussels, Gigantidas platifrons.SCIENCE OF THE TOTAL ENVIRONMENT,804,13.
MLA Sun, Yan,et al."Adaption to hydrogen sulfide-rich environments: Strategies for active detoxification in deep-sea symbiotic mussels, Gigantidas platifrons".SCIENCE OF THE TOTAL ENVIRONMENT 804(2022):13.
Files in This Item:
File Name/Size DocType Version Access License
1-s2.0-S004896972105(2987KB)期刊论文出版稿延迟开放CC BY-NC-SAView 2023-7-1后可获取
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Sun, Yan]'s Articles
[Wang, Minxiao]'s Articles
[Zhong, Zhaoshan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Sun, Yan]'s Articles
[Wang, Minxiao]'s Articles
[Zhong, Zhaoshan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Sun, Yan]'s Articles
[Wang, Minxiao]'s Articles
[Zhong, Zhaoshan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 1-s2.0-S0048969721051299-main.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.