Adaption to hydrogen sulfide-rich environments: Strategies for active detoxification in deep-sea symbiotic mussels, Gigantidas platifrons

doi:10.1016/j.scitotenv.2021.150054

	Adaption to hydrogen sulfide-rich environments: Strategies for active detoxification in deep-sea symbiotic mussels, Gigantidas platifrons
	Sun, Yan 1,2,3,4; Wang, Minxiao 1,2,3,4; Zhong, Zhaoshan1,2,3,4 ; Chen, Hao1,2,3,4 ; Wang, Hao 1,2,3,4; Zhou, Li 1,2,3,4; Cao, Lei1,2,3,4 ; Fu, Lulu 1,2,3,4; Zhang, Huan 1,2,3,4; Lian, Chao 1,2,3,4; Sun, Song1,2,3,4,5 ; Li, Chaolun1,2,3,4,5
	2022-01-15
发表期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
卷号	804 页码:13
通讯作者	Sun, Song(sunsong@qdio.ac.cn) ; Li, Chaolun(lcl@qdio.ac.cn)
摘要	The deep-sea mussel Gigantidas plantifrons is a representative species that relies on nutrition provided by chemoautotrophic endosymbiotic bacteria to survive in both hydrothermal vent and methane seep environments. However, vent and seep habitats have distinct geochemical features, with vents being more harsh than seeps because of abundant toxic chemical substances, particularly hydrogen sulfide (H2S). Until now, the adaptive strategies of G. platifrons in a heterogeneous environment and their sulfide detoxification mechanisms are still unclear. Herein, we conducted 16S rDNA sequencing and metatranscriptome sequencing of G. plan:trans collected from a methane seep at Formosa Ridge in the South China Sea and a hydrothermal vent at lheya North Knoll in the Mid-Okinawa Trough to provide a model for understanding environmental adaption and sulfide detoxification mechanisms, and a three-day laboratory controlled Na2S stress experiment to test the transcriptomic responses under sulfide stress. The results revealed the active detoxification of sulfide in G. platifrons gills. First, epibiotic Campylobacterota bacteria were more abundant in vent mussels and contributed to environmental adaptation by active oxidation of extracellular H2S. Notably, a key sulfide-oxidizing gene, sulfide:quinone oxidoreductase (sqr), derived from the methanotrophic endosymbiont, was significantly upregulated in vent mussels, indicating the oxidization of intracellular sulfide by the endosymbiont. In addition, transcriptomic comparison further suggested that genes involved in oxidative phosphorylation and mitochondrial sulfide oxidization pathway played important roles in the sulfide tolerance of the host mussels. Moreover, uanscriptomic analysis of Na2S stressed mussels confirmed the upregulation of oxidative phosphorylation and sulfide oxidization genes in response to sulfide exposure. Overall, this study provided a systematic transcriptional analysis of both the active bacterial community members and the host mussels, suggesting that the epibionts, endosymbionts, and mussel host collaborated on sulfide detoxification from extracellular to intracellular space to adapt to harsh H2S-rich environments. (C) 2021 Published by Elsevier B.V.
关键词	Sulfide detoxification Deep-sea adaptation Bathymodiolinae mussels Sulfide:quinone oxidoreductase Symbionts Mitochondria
DOI	10.1016/j.scitotenv.2021.150054
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2018YFC0310800] ; National Key Research and Development Program of China[2017YFC1404401] ; National Natural Science Foundation of China[42030407] ; National Natural Science Foundation of China[42076091] ; National Natural Science Foundation of China[41806200] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050303] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; Open Research Project of National Major Science & Technology Infrastructure[NMSTI-KEXUE2017K01] ; Open Research Project of National Major Science & Technology Infrastructure[KEXUE2021GH01] ; Open Research Project of National Major Science & Technology Infrastructure[KEXUE2019GZ02] ; Open Research Project of National Major Science & Technology Infrastructure[KEXUE2019GZ06] ; Open Research Project of National Major Science & Technology Infrastructure[KEXUE2018G16]
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000701855500014
出版者	ELSEVIER
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176492
专题	海洋生态与环境科学重点实验室
通讯作者	Sun, Song; Li, Chaolun
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室; 深海极端环境与生命过程研究中心; 中国科学院海洋大科学研究中心
通讯作者单位	海洋生态与环境科学重点实验室; 深海极端环境与生命过程研究中心; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Sun, Yan,Wang, Minxiao,Zhong, Zhaoshan,et al. Adaption to hydrogen sulfide-rich environments: Strategies for active detoxification in deep-sea symbiotic mussels, Gigantidas platifrons[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,804:13.
APA	Sun, Yan.,Wang, Minxiao.,Zhong, Zhaoshan.,Chen, Hao.,Wang, Hao.,...&Li, Chaolun.(2022).Adaption to hydrogen sulfide-rich environments: Strategies for active detoxification in deep-sea symbiotic mussels, Gigantidas platifrons.SCIENCE OF THE TOTAL ENVIRONMENT,804,13.
MLA	Sun, Yan,et al."Adaption to hydrogen sulfide-rich environments: Strategies for active detoxification in deep-sea symbiotic mussels, Gigantidas platifrons".SCIENCE OF THE TOTAL ENVIRONMENT 804(2022):13.

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