Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics
Song, Fengge1; Shen, Yuanyuan1; Wei, Yangdao1; Yang, Chunrong2; Ge, Xiaolin1; Wang, Aimin1; Li, Chaoyang1; Wan, Yi1,3; Li, Jinghong2
Corresponding AuthorWan, Yi(
AbstractThe rapid identification of pathogenic microorganisms plays a crucial role in the timely diagnosis and treatment strategies during a global pandemic, especially in resource-limited area. Herein, we present a sensitive biosensor strategy depended on botulinum neurotoxin type A light chain (BoNT/A LC) activated complex assay (BACA). BoNT/A LC, the surrogate of BoNT/A which embodying the most potent biological poisons, could serve as an ultrasensitive signal reporter with high signal-to-noise ratio to avoid common strong background response, poor stability and low intensity of current biosensor methods. A nanoparticle hybridization system, involving specific binding probes that recognize pathogenic 16S rRNAs or SARS-CoV-2 gene site, was developed to measure doublestranded biotinylated target DNA containing a single-stranded overhang using Fluorescence Resonance Energy Transfer (FRET)-based assay and colorimetric method. The method is validated widely by six different bacteria strains and severe acute respiratory related coronavirus 2 (SARS-CoV-2) nucleic acid, demonstrating a single cell or 1 aM nucleic acid detecting sensitivity. This detection strategy offers a solution for general applications and has a great prospect to be a simple instrument-free colorimetric tool, especially when facing public health emergency.
KeywordBacterial diagnostic SARS-CoV-2 Botulinum toxin Fluorescence resonance energy transfer Colorimetric detection Biosensors
Indexed BySCI
Funding ProjectKey Research and the Development Program of Hainan, China[ZDYF(XGFY)2020002] ; National Key Research and Development Program of China, China[2018YFD0900704] ; National Natural Science Foundation of China, China[41866002] ; Tsinghua University Initiative Scientific Research Program, China[2020Z99CFZ019] ; Shandong Key Laboratory of Corrosion Science, China[KLCS201910] ; Research Foundation of Hainan University, China[KYQD(ZR)1711]
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS IDWOS:000612675000002
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Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorWan, Yi
Affiliation1.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Key Lab Trop Biol Resources, Marine Coll,Minist Educ,Sch Life & Pharmaceut Sci, 56 Renmin Rd, Haikou 570228, Hainan, Peoples R China
2.Tsinghua Univ, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Dept Chem, Beijing 100084, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Shandong Key Lab Corros Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Song, Fengge,Shen, Yuanyuan,Wei, Yangdao,et al. Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics[J]. BIOSENSORS & BIOELECTRONICS,2021,176:8.
APA Song, Fengge.,Shen, Yuanyuan.,Wei, Yangdao.,Yang, Chunrong.,Ge, Xiaolin.,...&Li, Jinghong.(2021).Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics.BIOSENSORS & BIOELECTRONICS,176,8.
MLA Song, Fengge,et al."Botulinum toxin as an ultrasensitive reporter for bacterial and SARS-CoV-2 nucleic acid diagnostics".BIOSENSORS & BIOELECTRONICS 176(2021):8.
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