Transcriptome Analysis of the NematodeCaenorhabditis elegansin Acidic Stress Environments

doi:10.3389/fphys.2020.01107

IOCAS-IR

	Transcriptome Analysis of the NematodeCaenorhabditis elegansin Acidic Stress Environments
	Cong, Yanyi 1,2,3,4; Yang, Hanwen 1,2,3; Zhang, Pengchi 1,2,3,4; Xie, Yusu 1,2,3; Cao, Xuwen 1,2,3,4; Zhang, Liusuo 1,2,3
	2020-09-10
发表期刊	FRONTIERS IN PHYSIOLOGY
ISSN	1664-042X
卷号	11 页码:12
通讯作者	Zhang, Liusuo(lzhang@qdio.ac.cn)
摘要	Ocean acidification and acid rain, caused by modern industries' fossil fuel burning, lead to a decrease in the living environmental pH, which results in a series of negative effects on many organisms. However, the underlying mechanisms of animals' response to acidic pH stress are largely unknown. In this study, we used the nematodeCaenorhabditis elegansas an animal model to explore the regulatory mechanisms of organisms' response to pH decline. Two major stress-responsive pathways were found through transcriptome analysis in acidic stress environments. First, when the pH dropped from 6.33 to 4.33, the worms responded to the pH stress by upregulation of thecol,nas, anddpygenes, which are required for cuticle synthesis and structure integrity. Second, when the pH continued to decrease from 4.33, the metabolism of xenobiotics by cytochrome P450 pathway genes (cyp,gst,ugt, and ABC transporters) played a major role in protecting the nematodes from the toxic substances probably produced by the more acidic environment. At the same time, the slowing down of cuticle synthesis might be due to its insufficient protective ability. Moreover, the systematic regulation pattern we found in nematodes might also be applied to other invertebrate and vertebrate animals to survive in the changing pH environments. Thus, our data might lay the foundation to identify the master gene(s) responding and adapting to acidic pH stress in further studies, and might also provide new solutions to improve assessment and monitoring of ecological restoration outcomes, or generate novel genotypes via genome editing for restoring in challenging environments especially in the context of acidic stress through global climate change.
关键词	pH stress acidic environment cuticle collagens xenobiotic detoxification P450 Caenorhabditis elegans
DOI	10.3389/fphys.2020.01107
收录类别	SCI
语种	英语
资助项目	Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-1] ; Yong Scientist Research Program of Qingdao National Laboratory for Marine Science and Technology[TB2018NO01] ; Marine Life Breakthrough Funding of KEMBL, Chinese Academy of Sciences ; Talents From Overseas Program, IOCAS of the Chinese Academy of Sciences ; Qingdao Innovation Leadership Program[16-8-3-19-zhc] ; Key Deployment Project of Centre for Ocean MegaResearch of Science, Chinese Academy of Sciences ; NIH Office of Research Infrastructure Programs[P40 OD010440]
WOS研究方向	Physiology
WOS类目	Physiology
WOS记录号	WOS:000575386500001
出版者	FRONTIERS MEDIA SA
WOS关键词	NUCLEAR RECEPTOR SUPERFAMILY ; HEAT-SHOCK PROTEINS ; CAENORHABDITIS-ELEGANS ; ASTACIN METALLOPROTEASE ; OCEAN ACIDIFICATION ; C-ELEGANS ; RAIN ; PH ; HISAT ; DETOXIFICATION
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168869
通讯作者	Zhang, Liusuo
作者单位	1.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Cong, Yanyi,Yang, Hanwen,Zhang, Pengchi,et al. Transcriptome Analysis of the NematodeCaenorhabditis elegansin Acidic Stress Environments[J]. FRONTIERS IN PHYSIOLOGY,2020,11:12.
APA	Cong, Yanyi,Yang, Hanwen,Zhang, Pengchi,Xie, Yusu,Cao, Xuwen,&Zhang, Liusuo.(2020).Transcriptome Analysis of the NematodeCaenorhabditis elegansin Acidic Stress Environments.FRONTIERS IN PHYSIOLOGY,11,12.
MLA	Cong, Yanyi,et al."Transcriptome Analysis of the NematodeCaenorhabditis elegansin Acidic Stress Environments".FRONTIERS IN PHYSIOLOGY 11(2020):12.