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Pol-miR-363-3p plays a significant role in the immune defense of Japanese flounder Paralichthys olivaceus against bacterial and viral infection
Sun, Yan-Ling1,2; Guan, Xiao-Lu1,2; Zhang, Peng1,2,3; Li, Mo-Fei1,2; Zhang, Jian1,2; Sun, Li1,2
2020-09-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume104Pages:439-446
Corresponding AuthorSun, Li(lsun@qdio.ac.cn)
AbstractIn this study, we examined the function of a Japanese flounder (Paralichthys olivaceus) microRNA (miRNA), polmiR-363-3p. We found that pol-miR-363-3p targets an ubiquitin-specific protease (USP), USP32. USP is a family of deubiquitinating enzymes essential to the functioning of the ubiquitin proteasome system. In mammals, USP32 is known to be associated with cancer and immunity. In fish, the function of USP32 is unknown. We found that flounder USP32 (PoUSP32) expression was detected in the major tissues of flounder, particularly intestine. In vitro and in vivo studies showed that pol-miR-363-3p directly regulated PoUSP32 in a negative manner by interaction with the 3 ' UTR of PoUSP32. Overexpression of pol-miR-363-3p or interference with PoUSP32 expression in flounder cells significantly blocked Streptococcus iniae infection. Consistently, in vivo knockdown of pol-miR-363-3p or overexpression of PoUSP32 enhanced dissemination of S. iniae in flounder tissues, whereas in vivo knockdown of PoUSP32 inhibited S. iniae dissemination. In addition, pol-miR-363-3p knockdown also significantly promoted the tissue dissemination of the viral pathogen megalocytivirus, which, as well as S. iniae, regulated pol-miR-363-3p expression. Together these results revealed an important role of pol-miR-363-3p in flounder immune defense against bacterial and viral infection.
KeywordParalichthys olivaceus miRNA Ubiquitin-specific protease Pathogen Infection Immunity
DOI10.1016/j.fsi.2020.06.016
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31730100] ; National Natural Science Foundation of China[31902408] ; Marine S& T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; Taishan Scholar Program of Shandong Province
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000564306100002
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/168517
Collection实验海洋生物学重点实验室
Corresponding AuthorSun, Li
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Sun, Yan-Ling,Guan, Xiao-Lu,Zhang, Peng,et al. Pol-miR-363-3p plays a significant role in the immune defense of Japanese flounder Paralichthys olivaceus against bacterial and viral infection[J]. FISH & SHELLFISH IMMUNOLOGY,2020,104:439-446.
APA Sun, Yan-Ling,Guan, Xiao-Lu,Zhang, Peng,Li, Mo-Fei,Zhang, Jian,&Sun, Li.(2020).Pol-miR-363-3p plays a significant role in the immune defense of Japanese flounder Paralichthys olivaceus against bacterial and viral infection.FISH & SHELLFISH IMMUNOLOGY,104,439-446.
MLA Sun, Yan-Ling,et al."Pol-miR-363-3p plays a significant role in the immune defense of Japanese flounder Paralichthys olivaceus against bacterial and viral infection".FISH & SHELLFISH IMMUNOLOGY 104(2020):439-446.
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