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Functional characterization of T3SS C-ring component VscQ and evaluation of its mutant as a live attenuated vaccine in zebrafish (Danio rerio) model
Wu, Peiwen1,2,3,4; Zhou, Shihui1,2,3,4; Su, Zehui1,2,3,4; Liu, Chang1,2,3,4; Zeng, Fuyuan1,2,3,4; Pang, Huanying1,2,3,4,5,6; Xie, Miao1,2,3,4; Jian, Jichang1,2,3,4,5,6
2020-09-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume104Pages:123-132
Corresponding AuthorPang, Huanying(phying1218@163.com) ; Jian, Jichang(jianjic@gdou.edu.cn)
AbstractVibrio alginolyticus, a Gram-negative bacterium, has been recognized as an opportunistic pathogen in marine animals as well as humans. Type III secretion system (T3SS) is critical for pathogen virulence and disease development. However, no more information is known about the C-ring component VscQ and its physiological role. In this study, gene vscQ was cloned from V. alginolyticus wild-type strain HY9901 and the mutant strain HY9901 Delta vscQ was constructed by the in-frame deletion method. The HY9901 Delta vscQ mutant showed an attenuated swarming phenotype and a closely 4.6-fold decrease in the virulence to Danio rerio. However, the HY9901 Delta vscQ mutant showed no difference in growth, biofilm formation and ECPase activity. HY9901 Delta vscQ reduces the release of LDH, NO and caspase-3 activity of infected FHM cell, which are involved in fish cell apoptosis. Deletion of gene vscQ downregulates the expression level of T3SS-related genes including vscL, vopB, hop, vscO, vscK, vopD, vcrV and vopS and flagellum-related genes (flaA and fliG). And Danio rerio vaccinated via i.m injection with HY9901 Delta vscQ induced a relative percent survival (RPS) value of 71% after challenging with the wild-type HY9901. Real-time PCR assays showed that vaccination with HY9901 Delta vscQ enhanced the ex- pression of immune-related genes, including TNF-alpha, TLR5, IL-6R, IgM and c/ebp beta in liver and spleen after vaccination, indicating that it is able to induce humoral and cell-mediated immune response in zebrafish. These results demonstrate that the HY9901 Delta vscQ mutant could be used as an effective live vaccine to combat V. alginolyticus infection.
KeywordVibrio alginolyticus vscQ Live attenuated vaccines T3SS
DOI10.1016/j.fsi.2020.05.063
Indexed BySCI
Language英语
Funding ProjectIndependent Project of Southern Marine Science and Engineering Guangdong Laboratory[ZJW-2019-05] ; National Natural Science Foundation of China[31402344] ; Natural Science Foundation of Guangdong Province[2017A030313174] ; National Key R & Development Program of China[2018YFD0900501]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000564750000002
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/168511
Collection实验海洋生物学重点实验室
Corresponding AuthorPang, Huanying; Jian, Jichang
Affiliation1.Southern Marine Sci & Engn Guangdong Lab, Zhanjiang 524025, Peoples R China
2.Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524025, Peoples R China
3.Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524025, Peoples R China
4.Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang 524025, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
6.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wu, Peiwen,Zhou, Shihui,Su, Zehui,et al. Functional characterization of T3SS C-ring component VscQ and evaluation of its mutant as a live attenuated vaccine in zebrafish (Danio rerio) model[J]. FISH & SHELLFISH IMMUNOLOGY,2020,104:123-132.
APA Wu, Peiwen.,Zhou, Shihui.,Su, Zehui.,Liu, Chang.,Zeng, Fuyuan.,...&Jian, Jichang.(2020).Functional characterization of T3SS C-ring component VscQ and evaluation of its mutant as a live attenuated vaccine in zebrafish (Danio rerio) model.FISH & SHELLFISH IMMUNOLOGY,104,123-132.
MLA Wu, Peiwen,et al."Functional characterization of T3SS C-ring component VscQ and evaluation of its mutant as a live attenuated vaccine in zebrafish (Danio rerio) model".FISH & SHELLFISH IMMUNOLOGY 104(2020):123-132.
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