IOCAS-IR  > 实验海洋生物学重点实验室
Bisphenol A-associated alterations in DNA and histone methylation affects semen quality in rare minnow Gobiocypris rarus
Zhu, Long1; Yuan, Cong2; Wang, Mingrong1; Liu, Yan3; Wang, Zaizhao1; Seif, Mohamed M.1,4
2020-09-01
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume226Pages:11
Corresponding AuthorWang, Zaizhao(zzwang@nwsuaf.edu.cn)
AbstractBisphenol A (BPA), a well-known estrogenic endocrine disruptor, is ubiquitously present in the environment, possessing the potential to interfere with the reproductive endocrine system in male mammals. However, there are limited studies on the reproductive toxicity in male aquatic animals associated with epigenetic modifications. In order to evaluate the potential effects of BPA on reproduction and better understand the underlying mechanism, adult male rare minnow (Gobiocypris rarus) were exposed to 15 mu g L-1 BPA over a period of 63 d. Results showed that BPA induced congestion of blood vessels and infiltration of inflammatory cells after 21 d exposure, and decreased sperm fertilization after 63 d exposure. The genome DNA methylation levels were significantly increased throughout the treatment, and a strong positive stain were found in the spermatocyte, spermatid and sperm. The H3K4me3 level in all types of germ cell were increased by 21 d exposure while decreased following 63 d exposure. The positive stain of H3K9me3 was decreased in sperms while increased in spermatids by 21 d exposure. In addition, the H3K9me3 level was significantly increased after 63 d exposure, and a strong positive stain were found in spermatocytes, spermatids, and sperms. Our result also revealed that the transcripts of DNA methyltransferase genes (dnmt1 and dnmt3-8) and histone methyltransferase genes (mll2-5, setdb1-2 and ezh2) were also markedly changed under BPA exposure for 21-63 d. These findings indicated that BPA had toxicity in male reproductive, and DNA/histone methylation might play a vital role in the regulation of BPA-triggered the decreased of sperm quality.
KeywordBisphenol A DNA methylation Histone methylation Gobiocypris rarus
DOI10.1016/j.aquatox.2020.105580
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31670523]
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000565498500012
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/168478
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Zaizhao
Affiliation1.Northwest A&F Univ, Coll Anim Sci & Technol, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China
2.Lanzhou Univ, Sch Life Sci, Gansu Key Lab Biomonitoring & Bioremediat Environ, Lanzhou 730000, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
4.Natl Res Ctr, Toxicol & Food Contaminants Dept, Cairo 11435, Egypt
Recommended Citation
GB/T 7714
Zhu, Long,Yuan, Cong,Wang, Mingrong,et al. Bisphenol A-associated alterations in DNA and histone methylation affects semen quality in rare minnow Gobiocypris rarus[J]. AQUATIC TOXICOLOGY,2020,226:11.
APA Zhu, Long,Yuan, Cong,Wang, Mingrong,Liu, Yan,Wang, Zaizhao,&Seif, Mohamed M..(2020).Bisphenol A-associated alterations in DNA and histone methylation affects semen quality in rare minnow Gobiocypris rarus.AQUATIC TOXICOLOGY,226,11.
MLA Zhu, Long,et al."Bisphenol A-associated alterations in DNA and histone methylation affects semen quality in rare minnow Gobiocypris rarus".AQUATIC TOXICOLOGY 226(2020):11.
Files in This Item:
File Name/Size DocType Version Access License
Bisphenol A-associat(3794KB)期刊论文出版稿开放获取CC BY-NC-SAView
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhu, Long]'s Articles
[Yuan, Cong]'s Articles
[Wang, Mingrong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhu, Long]'s Articles
[Yuan, Cong]'s Articles
[Wang, Mingrong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhu, Long]'s Articles
[Yuan, Cong]'s Articles
[Wang, Mingrong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Bisphenol A-associated alterations in DNA and histone methylation affects semen quality in rare minnow Gobiocypris rarus.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.