A combined analysis of transcriptomics and proteomics of a novel Antarctic Salpa sp. and its potential toxin screenings
Ye, Ruiwei1,2; Liang, Hongyu1,2; Chen, Ting3; Zhang, Jie4; Yang, Fengling1,2; Chen, Xintong2; Li, Xiaoya2; Gao, Songyu2; Yang, Ying2,5; Wang, Yongqiang6; Tao, Feng2; Zhang, Jing1; Xiao, Liang2
Corresponding AuthorTao, Feng( ; Zhang, Jing( ; Xiao, Liang(
AbstractBackground: We successfully captured a kind of gelatinous organism DA-6 from Antarctic water, extracted its total RNAs and proteins, and performed species identification through a combination of transcriptomics and proteomics in this study. Methods: The gelatinous organism DA-6 was captured 200 m underwater in Antarctica. Total RNA was extracted to construct the transcriptome and the proteins were identified by LC-MS/MS. Results: DA-6 was identified as an Antarctic Salpa sp. through morphological examination and MT-CO1 phylogenetic analysis. A total of 47,183 unigenes were harvested through transcriptome. We also successfully annotated 11,954 (25.34%),10,006 (22.21%), 4469 (9.47%) and 4901 (9.71%) unigenes with NR, SwissProt, GO and KEGG databases, respectively. In the proteomic analysis, a total of 4680 peptides and 1280 proteins were harvested using the transcriptome as the reference database. A number of both 549 (31.98%) proteins reannotated against the GO and KEGG databases. Moreover, a number of 5 toxic proteins matched from the 89 toxin-related unigenes were successfully screened, including 2 metalloproteinases, 1 serine protease, 1 serine protease inhibitor and 1 aflatoxin. Conclusion: Our study is the first to identify an Antarctic Salpa sp. according to the combination of de novo transcriptomics and proteomics, which can further be served as a public database for the identification of potential polar Salpa-derived lead compounds. In addition to morphology and CO1, the combined analysis of transcriptome and proteome can also be used as a value method for new species identify e.g. Salpa sp. (C) 2020 Elsevier B.V. All rights reserved.
KeywordAntarctic Salpa Transcriptome Proteome Toxin
Indexed BySCI
Funding ProjectExcellent Youth Talent Program from Shanghai Municipal Commission of Health and Family Planning[2017YQ007] ; Military Youth Training Program Talent Project[18QNP016] ; National Natural Science Foundation of China[81770329]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS IDWOS:000564751600007
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Document Type期刊论文
Corresponding AuthorTao, Feng; Zhang, Jing; Xiao, Liang
Affiliation1.Jilin Agr Univ, Coll Tradit Chinese Med, Changchun 130118, Peoples R China
2.Second Mil Med Univ, Naval Med Univ, Fac Naval Med, Shanghai 200433, Peoples R China
3.Second Mil Med, Dept Cardiol, Changzheng Hosp, Affiliated Hosp 2,Naval Med Univ, Shanghai 200003, Peoples R China
4.Res Inst China, Polar Res Inst ChinaPolar, Shanghai 200433, Peoples R China
5.Shanxi Agr Univ, Coll Anim Sci & Vet Med, Taigu 030801, Shanxi, Peoples R China
6.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
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Ye, Ruiwei,Liang, Hongyu,Chen, Ting,et al. A combined analysis of transcriptomics and proteomics of a novel Antarctic Salpa sp. and its potential toxin screenings[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,160:1101-1113.
APA Ye, Ruiwei.,Liang, Hongyu.,Chen, Ting.,Zhang, Jie.,Yang, Fengling.,...&Xiao, Liang.(2020).A combined analysis of transcriptomics and proteomics of a novel Antarctic Salpa sp. and its potential toxin screenings.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,160,1101-1113.
MLA Ye, Ruiwei,et al."A combined analysis of transcriptomics and proteomics of a novel Antarctic Salpa sp. and its potential toxin screenings".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 160(2020):1101-1113.
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