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A combined analysis of transcriptomics and proteomics of a novel Antarctic Salpa sp. and its potential toxin screenings
Ye, Ruiwei1,2; Liang, Hongyu1,2; Chen, Ting3; Zhang, Jie4; Yang, Fengling1,2; Chen, Xintong2; Li, Xiaoya2; Gao, Songyu2; Yang, Ying2,5; Wang, Yongqiang6; Tao, Feng2; Zhang, Jing1; Xiao, Liang2
2020-10-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume160Pages:1101-1113
Corresponding AuthorTao, Feng(tf850706@163.com) ; Zhang, Jing(zhjing0701@163.com) ; Xiao, Liang(hormat830713@hotmail.com)
AbstractBackground: We successfully captured a kind of gelatinous organism DA-6 from Antarctic water, extracted its total RNAs and proteins, and performed species identification through a combination of transcriptomics and proteomics in this study. Methods: The gelatinous organism DA-6 was captured 200 m underwater in Antarctica. Total RNA was extracted to construct the transcriptome and the proteins were identified by LC-MS/MS. Results: DA-6 was identified as an Antarctic Salpa sp. through morphological examination and MT-CO1 phylogenetic analysis. A total of 47,183 unigenes were harvested through transcriptome. We also successfully annotated 11,954 (25.34%),10,006 (22.21%), 4469 (9.47%) and 4901 (9.71%) unigenes with NR, SwissProt, GO and KEGG databases, respectively. In the proteomic analysis, a total of 4680 peptides and 1280 proteins were harvested using the transcriptome as the reference database. A number of both 549 (31.98%) proteins reannotated against the GO and KEGG databases. Moreover, a number of 5 toxic proteins matched from the 89 toxin-related unigenes were successfully screened, including 2 metalloproteinases, 1 serine protease, 1 serine protease inhibitor and 1 aflatoxin. Conclusion: Our study is the first to identify an Antarctic Salpa sp. according to the combination of de novo transcriptomics and proteomics, which can further be served as a public database for the identification of potential polar Salpa-derived lead compounds. In addition to morphology and CO1, the combined analysis of transcriptome and proteome can also be used as a value method for new species identify e.g. Salpa sp. (C) 2020 Elsevier B.V. All rights reserved.
KeywordAntarctic Salpa Transcriptome Proteome Toxin
DOI10.1016/j.ijbiomac.2020.05.144
Indexed BySCI
Language英语
Funding ProjectExcellent Youth Talent Program from Shanghai Municipal Commission of Health and Family Planning[2017YQ007] ; Military Youth Training Program Talent Project[18QNP016] ; National Natural Science Foundation of China[81770329]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS IDWOS:000564751600007
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/168475
Collection中国科学院海洋研究所
Corresponding AuthorTao, Feng; Zhang, Jing; Xiao, Liang
Affiliation1.Jilin Agr Univ, Coll Tradit Chinese Med, Changchun 130118, Peoples R China
2.Second Mil Med Univ, Naval Med Univ, Fac Naval Med, Shanghai 200433, Peoples R China
3.Second Mil Med, Dept Cardiol, Changzheng Hosp, Affiliated Hosp 2,Naval Med Univ, Shanghai 200003, Peoples R China
4.Res Inst China, Polar Res Inst ChinaPolar, Shanghai 200433, Peoples R China
5.Shanxi Agr Univ, Coll Anim Sci & Vet Med, Taigu 030801, Shanxi, Peoples R China
6.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Ye, Ruiwei,Liang, Hongyu,Chen, Ting,et al. A combined analysis of transcriptomics and proteomics of a novel Antarctic Salpa sp. and its potential toxin screenings[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,160:1101-1113.
APA Ye, Ruiwei.,Liang, Hongyu.,Chen, Ting.,Zhang, Jie.,Yang, Fengling.,...&Xiao, Liang.(2020).A combined analysis of transcriptomics and proteomics of a novel Antarctic Salpa sp. and its potential toxin screenings.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,160,1101-1113.
MLA Ye, Ruiwei,et al."A combined analysis of transcriptomics and proteomics of a novel Antarctic Salpa sp. and its potential toxin screenings".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 160(2020):1101-1113.
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