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Effects of a Sudden Drop in Salinity on Scapharca subcrenata Antioxidant Defenses and Metabolism Determined Using LC-MS Non-targeted Metabolomics
Zhang, Mo1,2; Li, Li3; Liu, Ying4; Gao, Xiaolong1
2020-04-30
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume10Issue:1Pages:14
Corresponding AuthorGao, Xiaolong(xlgao@xmu.edu.cn)
AbstractIn this experiment, the effects of a sudden drop in salinity on the antioxidant defense system and related gene expression of the ark shell Scapharca subcrenata were examined. The sudden drop in seawater salinity after a rainstorm was simulated, and subsequently differentially expressed metabolic markers were identified by LC-MS non-targeted metabolomics. When the salinity dropped to 14 parts per thousand (S14), the total anti-oxidant content, activity of Na+/K+-ATPase, superoxide dismutase (SOD), and catalase (CAT), content of malondialdehyde, and expression levels of Mn-SOD, CAT, and C-type lectin of S. subcrenata were significantly higher than in groups with salinity of 22 parts per thousand (S22) or 30 parts per thousand (S30) (P<0.05). The activity of glutathione peroxidase (GPx), the content of reduced glutathione, and the expression levels of GP(x) were not significantly different between S14 and S22, but the values in each group were significantly higher than those in S30 (P<0.05). Using the metabolomics technique, 361, 271, and 264 metabolites with significant differences were identified from S22 vs. S14, S30 vs. S14, and S30 vs. S22, respectively. The drop in salinity was accompanied by up-regulation of phosphatidylcholine (PC) (20:4 (5Z, 8Z, 11Z, 14Z)/P-18: 1 (11Z)), PC (16:0/22: 6 (4Z, 7Z, 10Z, 13Z, 16Z, 19Z)), phosphatidylethanolamine (PE) (18:4 (6Z, 9Z, 12Z, 15Z)/24:1 (15Z)), phosphatidylinositol (PI) (20:1 (11Z)/0:0), phalluside-1, C16 sphinganine, and LacCer (d18:0/14:0) and by significant down-regulation of PI-Cer (d18:1/14:0) and PE (14:0/16:1(9Z). The results of this study illustrate how these nine metabolites can be used as metabolic markers for the response of S. subcrenata to a sudden drop in salinity. They also provide the theoretical groundwork for selection of bottom areas with salinity that is optimal for release and proliferation of S. subcrenata, which is needed to restore the declining populations of this species.
DOI10.1038/s41598-020-63293-0
Indexed BySCI
Language英语
Funding ProjectChina Postdoctoral Science Foundation[2019M650153] ; Modern Agro-industry Technology Research System in Shandong Province[SDAIT-14-04] ; Outstanding Postdoctoral Scholarship from the State Key Laboratory of Marine Environmental Science at Xiamen University
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000559954900001
PublisherNATURE PUBLISHING GROUP
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/168193
Collection实验海洋生物学重点实验室
Corresponding AuthorGao, Xiaolong
Affiliation1.Xiamen Univ, Coll Ocean & Earth Sci, State Key Lab Marine Environm Sci, Xiamen 361102, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.China Marine Biol Inst Shandong Prov, Qingdao 266104, Peoples R China
4.Dalian Ocean Univ, Dalian 116023, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Mo,Li, Li,Liu, Ying,et al. Effects of a Sudden Drop in Salinity on Scapharca subcrenata Antioxidant Defenses and Metabolism Determined Using LC-MS Non-targeted Metabolomics[J]. SCIENTIFIC REPORTS,2020,10(1):14.
APA Zhang, Mo,Li, Li,Liu, Ying,&Gao, Xiaolong.(2020).Effects of a Sudden Drop in Salinity on Scapharca subcrenata Antioxidant Defenses and Metabolism Determined Using LC-MS Non-targeted Metabolomics.SCIENTIFIC REPORTS,10(1),14.
MLA Zhang, Mo,et al."Effects of a Sudden Drop in Salinity on Scapharca subcrenata Antioxidant Defenses and Metabolism Determined Using LC-MS Non-targeted Metabolomics".SCIENTIFIC REPORTS 10.1(2020):14.
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