Effects of a Sudden Drop in Salinity on Scapharca subcrenata Antioxidant Defenses and Metabolism Determined Using LC-MS Non-targeted Metabolomics

doi:10.1038/s41598-020-63293-0

IOCAS-IR > 实验海洋生物学重点实验室

	Effects of a Sudden Drop in Salinity on Scapharca subcrenata Antioxidant Defenses and Metabolism Determined Using LC-MS Non-targeted Metabolomics
	Zhang, Mo 1,2; Li, Li 3; Liu, Ying4 ; Gao, Xiaolong 1
	2020-04-30
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	10 期号:1 页码:14
通讯作者	Gao, Xiaolong(xlgao@xmu.edu.cn)
摘要	In this experiment, the effects of a sudden drop in salinity on the antioxidant defense system and related gene expression of the ark shell Scapharca subcrenata were examined. The sudden drop in seawater salinity after a rainstorm was simulated, and subsequently differentially expressed metabolic markers were identified by LC-MS non-targeted metabolomics. When the salinity dropped to 14 parts per thousand (S14), the total anti-oxidant content, activity of Na+/K+-ATPase, superoxide dismutase (SOD), and catalase (CAT), content of malondialdehyde, and expression levels of Mn-SOD, CAT, and C-type lectin of S. subcrenata were significantly higher than in groups with salinity of 22 parts per thousand (S22) or 30 parts per thousand (S30) (P<0.05). The activity of glutathione peroxidase (GPx), the content of reduced glutathione, and the expression levels of GP(x) were not significantly different between S14 and S22, but the values in each group were significantly higher than those in S30 (P<0.05). Using the metabolomics technique, 361, 271, and 264 metabolites with significant differences were identified from S22 vs. S14, S30 vs. S14, and S30 vs. S22, respectively. The drop in salinity was accompanied by up-regulation of phosphatidylcholine (PC) (20:4 (5Z, 8Z, 11Z, 14Z)/P-18: 1 (11Z)), PC (16:0/22: 6 (4Z, 7Z, 10Z, 13Z, 16Z, 19Z)), phosphatidylethanolamine (PE) (18:4 (6Z, 9Z, 12Z, 15Z)/24:1 (15Z)), phosphatidylinositol (PI) (20:1 (11Z)/0:0), phalluside-1, C16 sphinganine, and LacCer (d18:0/14:0) and by significant down-regulation of PI-Cer (d18:1/14:0) and PE (14:0/16:1(9Z). The results of this study illustrate how these nine metabolites can be used as metabolic markers for the response of S. subcrenata to a sudden drop in salinity. They also provide the theoretical groundwork for selection of bottom areas with salinity that is optimal for release and proliferation of S. subcrenata, which is needed to restore the declining populations of this species.
DOI	10.1038/s41598-020-63293-0
收录类别	SCI
语种	英语
资助项目	China Postdoctoral Science Foundation[2019M650153] ; Modern Agro-industry Technology Research System in Shandong Province[SDAIT-14-04] ; Outstanding Postdoctoral Scholarship from the State Key Laboratory of Marine Environmental Science at Xiamen University
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000559954900001
出版者	NATURE PUBLISHING GROUP
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168193
专题	实验海洋生物学重点实验室
通讯作者	Gao, Xiaolong
作者单位	1.Xiamen Univ, Coll Ocean & Earth Sci, State Key Lab Marine Environm Sci, Xiamen 361102, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.China Marine Biol Inst Shandong Prov, Qingdao 266104, Peoples R China 4.Dalian Ocean Univ, Dalian 116023, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Zhang, Mo,Li, Li,Liu, Ying,et al. Effects of a Sudden Drop in Salinity on Scapharca subcrenata Antioxidant Defenses and Metabolism Determined Using LC-MS Non-targeted Metabolomics[J]. SCIENTIFIC REPORTS,2020,10(1):14.
APA	Zhang, Mo,Li, Li,Liu, Ying,&Gao, Xiaolong.(2020).Effects of a Sudden Drop in Salinity on Scapharca subcrenata Antioxidant Defenses and Metabolism Determined Using LC-MS Non-targeted Metabolomics.SCIENTIFIC REPORTS,10(1),14.
MLA	Zhang, Mo,et al."Effects of a Sudden Drop in Salinity on Scapharca subcrenata Antioxidant Defenses and Metabolism Determined Using LC-MS Non-targeted Metabolomics".SCIENTIFIC REPORTS 10.1(2020):14.

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