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The Translocation and Assembly Module (TAM) ofEdwardsiella tardaIs Essential for Stress Resistance and Host Infection
Li, Mo-fei1,2; Jia, Bei-bei1,2,3; Sun, Yuan-yuan1,2; Sun, Li1,2
2020-07-24
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
Volume11Pages:12
Corresponding AuthorSun, Li(lsun@qdio.ac.cn)
AbstractTranslocation and assembly module (TAM) is a protein channel known to mediate the secretion of virulence factors during pathogen infection.Edwardsiella tardais a Gram-negative bacterium that is pathogenic to a wide range of farmed fish and other hosts including humans. In this study, we examined the function of the two components of the TAM, TamA and TamB, ofE. tarda(namedtamA(Et)andtamB(Et), respectively). TamA(Et)was found to localize on the surface ofE. tardaand be recognizable by TamA(Et)antibody. Compared to the wild type, thetamAandtamBknockouts, TX01 Delta tamAand TX01 Delta tamB, respectively, were significantly reduced in motility, flagella formation, invasion into host cells, intracellular replication, dissemination in host tissues, and inducing host mortality. The lost virulence capacities of TX01 Delta tamAand TX01 Delta tamBwere restored by complementation with thetamA(Et)andtamB(Et)genes, respectively. Furthermore, TX01 Delta tamAand TX01 Delta tamBwere significantly impaired in the ability to survive under low pH and oxidizing conditions, and were unable to maintain their internal pH balance and cellular structures in acidic environments, which led to increased susceptibility to lysozyme destruction. Taken together, these results indicate that TamA(Et)and TamB(Et)are essential for the virulence ofE. tardaand required forE. tardato survive under stress conditions.
KeywordEdwardsiella tarda translocation and assembly module acid tolerance membrane integrity virulence
DOI10.3389/fmicb.2020.01743
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2018YFD0900501] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; National Natural Science Foundation of China[31972831] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2017249] ; Huiquan Young Scholar Program of Institute of Oceanology, CAS ; Taishan Scholar Program of Shandong Province
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000560547400001
PublisherFRONTIERS MEDIA SA
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/168154
Collection实验海洋生物学重点实验室
Corresponding AuthorSun, Li
Affiliation1.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Mo-fei,Jia, Bei-bei,Sun, Yuan-yuan,et al. The Translocation and Assembly Module (TAM) ofEdwardsiella tardaIs Essential for Stress Resistance and Host Infection[J]. FRONTIERS IN MICROBIOLOGY,2020,11:12.
APA Li, Mo-fei,Jia, Bei-bei,Sun, Yuan-yuan,&Sun, Li.(2020).The Translocation and Assembly Module (TAM) ofEdwardsiella tardaIs Essential for Stress Resistance and Host Infection.FRONTIERS IN MICROBIOLOGY,11,12.
MLA Li, Mo-fei,et al."The Translocation and Assembly Module (TAM) ofEdwardsiella tardaIs Essential for Stress Resistance and Host Infection".FRONTIERS IN MICROBIOLOGY 11(2020):12.
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