Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| The Translocation and Assembly Module (TAM) ofEdwardsiella tardaIs Essential for Stress Resistance and Host Infection | |
Li, Mo-fei1,2 ; Jia, Bei-bei1,2,3; Sun, Yuan-yuan1,2; Sun, Li1,2
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| 2020-07-24 | |
| 发表期刊 | FRONTIERS IN MICROBIOLOGY
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| ISSN | 1664-302X |
| 卷号 | 11页码:12 |
| 通讯作者 | Sun, Li(lsun@qdio.ac.cn) |
| 摘要 | Translocation and assembly module (TAM) is a protein channel known to mediate the secretion of virulence factors during pathogen infection.Edwardsiella tardais a Gram-negative bacterium that is pathogenic to a wide range of farmed fish and other hosts including humans. In this study, we examined the function of the two components of the TAM, TamA and TamB, ofE. tarda(namedtamA(Et)andtamB(Et), respectively). TamA(Et)was found to localize on the surface ofE. tardaand be recognizable by TamA(Et)antibody. Compared to the wild type, thetamAandtamBknockouts, TX01 Delta tamAand TX01 Delta tamB, respectively, were significantly reduced in motility, flagella formation, invasion into host cells, intracellular replication, dissemination in host tissues, and inducing host mortality. The lost virulence capacities of TX01 Delta tamAand TX01 Delta tamBwere restored by complementation with thetamA(Et)andtamB(Et)genes, respectively. Furthermore, TX01 Delta tamAand TX01 Delta tamBwere significantly impaired in the ability to survive under low pH and oxidizing conditions, and were unable to maintain their internal pH balance and cellular structures in acidic environments, which led to increased susceptibility to lysozyme destruction. Taken together, these results indicate that TamA(Et)and TamB(Et)are essential for the virulence ofE. tardaand required forE. tardato survive under stress conditions. |
| 关键词 | Edwardsiella tarda translocation and assembly module acid tolerance membrane integrity virulence |
| DOI | 10.3389/fmicb.2020.01743 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Key Research and Development Program of China[2018YFD0900501] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; National Natural Science Foundation of China[31972831] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2017249] ; Huiquan Young Scholar Program of Institute of Oceanology, CAS ; Taishan Scholar Program of Shandong Province |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| WOS记录号 | WOS:000560547400001 |
| 出版者 | FRONTIERS MEDIA SA |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/168154 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Li |
| 作者单位 | 1.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 第一作者单位 | 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Li, Mo-fei,Jia, Bei-bei,Sun, Yuan-yuan,et al. The Translocation and Assembly Module (TAM) ofEdwardsiella tardaIs Essential for Stress Resistance and Host Infection[J]. FRONTIERS IN MICROBIOLOGY,2020,11:12. |
| APA | Li, Mo-fei,Jia, Bei-bei,Sun, Yuan-yuan,&Sun, Li.(2020).The Translocation and Assembly Module (TAM) ofEdwardsiella tardaIs Essential for Stress Resistance and Host Infection.FRONTIERS IN MICROBIOLOGY,11,12. |
| MLA | Li, Mo-fei,et al."The Translocation and Assembly Module (TAM) ofEdwardsiella tardaIs Essential for Stress Resistance and Host Infection".FRONTIERS IN MICROBIOLOGY 11(2020):12. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| The Translocation an（3521KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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