Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
The cyclic lipopeptides suppress the motility ofVibrio alginolyticusvia targeting the Na+-driven flagellar motor componentMotX | |
Liu, Rui1,2,3; Zheng, Rikuan1,2,3,4; Liu, Ge1,2,3; Sun, Chaomin1,2,3 | |
2020-07-28 | |
发表期刊 | ENVIRONMENTAL MICROBIOLOGY |
ISSN | 1462-2912 |
页码 | 14 |
通讯作者 | Sun, Chaomin(sunchaomin@qdio.ac.cn) |
摘要 | In our previous study, we found that pumilacidin-like cyclic lipopeptides (CLPs) derived from marine bacteriumBacillussp. strain 176 significantly suppressed the mobile capability and virulence ofVibrio alginolyticus. Here, to further disclose the mechanism of CLPs inhibiting the motility ofV.alginolyticus, we first applied transcriptomic analysis toV.alginolyticustreated with or without CLPs. The transcriptomic results showed that the expression of several important components of the Na+-driven flagellar motor closely related to bacterial motility were markedly suppressed, suggesting that the structure and function of Na+-driven flagellar motor might be disabled by CLPs. The transcriptomic data were further analysed by the protein-protein interaction network, and the results supported that MotX, one of the essential components of Na+-driven flagellar motor was most likely the action target of CLPs. In combination of gene knockout, electrophoretic mobility shift assay and immunoblotting techniques, CLPs were demonstrated to affect the rotation of flagella ofVibrio alginolyticusvia direct interacting with the Na+-driven flagellar motor component MotX, which eventually inhibited the bacterial motility. Interestingly, homologues of MotX were found broadly distributed and highly conserved in different pathogenic species, which extends the application range of CLPs as an antibacterial drug targeting bacterial motility in many pathogens. |
DOI | 10.1111/1462-2920.15144 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Key R and D Program of China[2018YFC0310800] ; Young Scientists Fund of LMBB[YQ2018NO02] ; Qingdao National Laboratory for Marine Science and Technology ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051] ; Qingdao Innovation Leadership Program[18-1-2-7-zhc] ; China Ocean Mineral Resources R&D Association Grant[DY135-B2-14] |
WOS研究方向 | Microbiology |
WOS类目 | Microbiology |
WOS记录号 | WOS:000552750800001 |
出版者 | WILEY |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/167988 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Sun, Chaomin |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Liu, Rui,Zheng, Rikuan,Liu, Ge,et al. The cyclic lipopeptides suppress the motility ofVibrio alginolyticusvia targeting the Na+-driven flagellar motor componentMotX[J]. ENVIRONMENTAL MICROBIOLOGY,2020:14. |
APA | Liu, Rui,Zheng, Rikuan,Liu, Ge,&Sun, Chaomin.(2020).The cyclic lipopeptides suppress the motility ofVibrio alginolyticusvia targeting the Na+-driven flagellar motor componentMotX.ENVIRONMENTAL MICROBIOLOGY,14. |
MLA | Liu, Rui,et al."The cyclic lipopeptides suppress the motility ofVibrio alginolyticusvia targeting the Na+-driven flagellar motor componentMotX".ENVIRONMENTAL MICROBIOLOGY (2020):14. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
The cyclic lipopepti(3344KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 |
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