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The cyclic lipopeptides suppress the motility ofVibrio alginolyticusvia targeting the Na+-driven flagellar motor componentMotX
Liu, Rui1,2,3; Zheng, Rikuan1,2,3,4; Liu, Ge1,2,3; Sun, Chaomin1,2,3
2020-07-28
Source PublicationENVIRONMENTAL MICROBIOLOGY
ISSN1462-2912
Pages14
Corresponding AuthorSun, Chaomin(sunchaomin@qdio.ac.cn)
AbstractIn our previous study, we found that pumilacidin-like cyclic lipopeptides (CLPs) derived from marine bacteriumBacillussp. strain 176 significantly suppressed the mobile capability and virulence ofVibrio alginolyticus. Here, to further disclose the mechanism of CLPs inhibiting the motility ofV.alginolyticus, we first applied transcriptomic analysis toV.alginolyticustreated with or without CLPs. The transcriptomic results showed that the expression of several important components of the Na+-driven flagellar motor closely related to bacterial motility were markedly suppressed, suggesting that the structure and function of Na+-driven flagellar motor might be disabled by CLPs. The transcriptomic data were further analysed by the protein-protein interaction network, and the results supported that MotX, one of the essential components of Na+-driven flagellar motor was most likely the action target of CLPs. In combination of gene knockout, electrophoretic mobility shift assay and immunoblotting techniques, CLPs were demonstrated to affect the rotation of flagella ofVibrio alginolyticusvia direct interacting with the Na+-driven flagellar motor component MotX, which eventually inhibited the bacterial motility. Interestingly, homologues of MotX were found broadly distributed and highly conserved in different pathogenic species, which extends the application range of CLPs as an antibacterial drug targeting bacterial motility in many pathogens.
DOI10.1111/1462-2920.15144
Indexed BySCI
Language英语
Funding ProjectNational Key R and D Program of China[2018YFC0310800] ; Young Scientists Fund of LMBB[YQ2018NO02] ; Qingdao National Laboratory for Marine Science and Technology ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051] ; Qingdao Innovation Leadership Program[18-1-2-7-zhc] ; China Ocean Mineral Resources R&D Association Grant[DY135-B2-14]
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000552750800001
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167988
Collection实验海洋生物学重点实验室
Corresponding AuthorSun, Chaomin
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Rui,Zheng, Rikuan,Liu, Ge,et al. The cyclic lipopeptides suppress the motility ofVibrio alginolyticusvia targeting the Na+-driven flagellar motor componentMotX[J]. ENVIRONMENTAL MICROBIOLOGY,2020:14.
APA Liu, Rui,Zheng, Rikuan,Liu, Ge,&Sun, Chaomin.(2020).The cyclic lipopeptides suppress the motility ofVibrio alginolyticusvia targeting the Na+-driven flagellar motor componentMotX.ENVIRONMENTAL MICROBIOLOGY,14.
MLA Liu, Rui,et al."The cyclic lipopeptides suppress the motility ofVibrio alginolyticusvia targeting the Na+-driven flagellar motor componentMotX".ENVIRONMENTAL MICROBIOLOGY (2020):14.
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