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Identification and expression characterization of a novel carboxypeptidase A-like gene (RvCPA) during early developmental stages in the gastropod Rapana venosa (Muricidae)
Yang, Mei-Jie1,3,4; Song, Hao1,2,4; Yu, Zheng-Lin1,2,4; Xu, Tao5; Hu, Zhi1,3,4; Zhou, Cong1,3,4; Shi, Pu1,3,4; Zhang, Tao1,2,4
2020-07-01
Source PublicationAQUACULTURE REPORTS
ISSN2352-5134
Volume17Pages:8
Corresponding AuthorZhang, Tao(tzhang@qdio.ac.cn)
AbstractRapana venosa is an economically important aquaculture species in China. Carboxypeptidases (CPs) A and B (CPA and CPB, respectively) hydrolyze peptides and proteins and play critical roles in the development of the digestive system in animals. In this study, a full-length CPA-like cDNA sequence from R. venosa (RvCPA) was isolated using RACE, and RvCPA mRNA expression levels were analyzed in different tissues and developmental stages by quantitative real-time PCR. Additionally, the expression patterns of RvCPA under different diets were investigated in competent larvae of R. venosa. The full-length cDNA of the RvCPA gene was 1,810 bp and consisted of a 1,296-bp open reading frame (ORF) encoding 431 aa. Phylogenetic analysis revealed high homology of RvCPA with CPs from Biomphalaria glabrata, Pomacea canaliculata, and Ampullaria crossean. Our results showed that RvCPA had higher expression in the salivary gland than in other tissues and that the expression of RvCPA was dramatically increased in the juvenile (postlarval) stage compared with other stages, indicating that functional changes occur in the larval digestive system during the food habit transition. Furthermore, we found that an animal diet could significantly increase the expression of RvCPA, which may suggest that animal diets can promote the development of the digestive system. This information on a novel CP in R. venosa that participates in digestion and is related to the food habit transition may broaden our understanding of digestive system development during metamorphosis in carnivorous gastropods.
KeywordRapana venosa carboxypeptidase carnivorous food habit transition gastropod
DOI10.1016/j.aqrep.2020.100360
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2019YFD0900800] ; National Natural Science Foundation of China[31572636] ; National Natural Science Foundation of China[31972814] ; Natural Science Foundation of Shandong Province[ZR2019BD003] ; China Postdoctoral Science Foundation[2019M652498] ; Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program[2019JZZY020708] ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49] ; Industry Leading Talents Project of Taishan Scholars ; `Double Hundred' Blue Industry Leader Team of Yantai ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology[LMEESCTSP-2018-1]
WOS Research AreaFisheries
WOS SubjectFisheries
WOS IDWOS:000544612200010
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167916
Collection海洋生态与环境科学重点实验室
Corresponding AuthorZhang, Tao
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Sci & Technol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
5.Shandong Fisheries Technol Extens Ctr, Jinan 250013, Peoples R China
First Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yang, Mei-Jie,Song, Hao,Yu, Zheng-Lin,et al. Identification and expression characterization of a novel carboxypeptidase A-like gene (RvCPA) during early developmental stages in the gastropod Rapana venosa (Muricidae)[J]. AQUACULTURE REPORTS,2020,17:8.
APA Yang, Mei-Jie.,Song, Hao.,Yu, Zheng-Lin.,Xu, Tao.,Hu, Zhi.,...&Zhang, Tao.(2020).Identification and expression characterization of a novel carboxypeptidase A-like gene (RvCPA) during early developmental stages in the gastropod Rapana venosa (Muricidae).AQUACULTURE REPORTS,17,8.
MLA Yang, Mei-Jie,et al."Identification and expression characterization of a novel carboxypeptidase A-like gene (RvCPA) during early developmental stages in the gastropod Rapana venosa (Muricidae)".AQUACULTURE REPORTS 17(2020):8.
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